ID   CISY_RICAE              Reviewed;         435 AA.
AC   Q59732; C3PLX9;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=RAF_ORF1196;
OS   Rickettsia africae (strain ESF-5).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=347255;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ESF-5;
RX   PubMed=19379498; DOI=10.1186/1471-2164-10-166;
RA   Fournier P.-E., El Karkouri K., Leroy Q., Robert C., Giumelli B.,
RA   Renesto P., Socolovschi C., Parola P., Audic S., Raoult D.;
RT   "Analysis of the Rickettsia africae genome reveals that virulence
RT   acquisition in Rickettsia species may be explained by genome reduction.";
RL   BMC Genomics 10:166-166(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-418.
RX   PubMed=9103608; DOI=10.1099/00207713-47-2-252;
RA   Roux V., Rydkina E., Eremeeva M., Raoult D.;
RT   "Citrate synthase gene comparison, a new tool for phylogenetic analysis,
RT   and its application for the rickettsiae.";
RL   Int. J. Syst. Bacteriol. 47:252-261(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; CP001612; ACP53969.1; -; Genomic_DNA.
DR   EMBL; U59733; AAB02955.1; -; Genomic_DNA.
DR   RefSeq; WP_012720089.1; NC_012633.1.
DR   AlphaFoldDB; Q59732; -.
DR   SMR; Q59732; -.
DR   EnsemblBacteria; ACP53969; ACP53969; RAF_ORF1196.
DR   KEGG; raf:RAF_ORF1196; -.
DR   HOGENOM; CLU_025068_0_0_5; -.
DR   OMA; TVGWCAQ; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000002305; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..435
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169956"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        370
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   CONFLICT        196
FT                   /note="S -> P (in Ref. 2; AAB02955)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   435 AA;  49205 MW;  F807EEE46926FFA2 CRC64;
     MTDENNNDSE FAELKIRGKI FKLPILKASI GEDVIDISRV SAEADCFTYD PGFMSTASCQ
     STITYIDGDK GILRHRGYDI KDLAEKSDFL EVAYLLIYGE LPSGEQYNNF TKQVAHHSLV
     NERLHYLFQT FCSSSHPMAI MLAAVGSLSA FYPDLLNFKE ADYELTAIRM IAKIPTIAAM
     SYKYSIGQPF IYPDNSLDFT ENFLHMMFAT PCTKYTVNPI IKNALNKIFI LHADHEQNAS
     TSTVRIAGSS GANPFACIST GIASLWGPAH GGANEAVINM LKEIGSSEYI PKYIAKAKDK
     NDPFRLMGFG HRVYKNYDPR AAVLKETCKE VLKELGQLDN NPLLQIAIEL EAIALKDEYF
     IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FVIARTVGWM AQWKEMHEDP EQKISRPRQL
     YTGYVHREYK GIRER