ID   CISY_RICAK              Reviewed;         411 AA.
AC   Q59730; Q59729;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
DE   Flags: Fragment;
GN   Name=gltA;
OS   Rickettsia akari.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=786;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MK / Kaplan;
RX   PubMed=9103608; DOI=10.1099/00207713-47-2-252;
RA   Roux V., Rydkina E., Eremeeva M., Raoult D.;
RT   "Citrate synthase gene comparison, a new tool for phylogenetic analysis,
RT   and its application for the rickettsiae.";
RL   Int. J. Syst. Bacteriol. 47:252-261(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 259-385.
RC   STRAIN=MK / Kaplan;
RA   Azad A.F., Higgins J.A., Radulovic S., Schriefer M.E.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; U59717; AAB02953.1; -; Genomic_DNA.
DR   EMBL; U41752; AAB49577.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q59730; -.
DR   SMR; Q59730; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           <1..>411
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169957"
FT   ACT_SITE        304
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        363
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   NON_TER         1
FT   NON_TER         411
SQ   SEQUENCE   411 AA;  46173 MW;  8FEBC66C3CB5FB11 CRC64;
     DSEFAELKIR GKIFQLPILK ASIGEDVIDI SRVSAEVNCF TYDPGFMSTA SCKSTITYID
     GDKGILRHRG YNIKDLAEKS DFLEVAYLLI YGELPSIEQY NNFTKQVAHH SLVNERLHYL
     FQTFCSSSHP MAIMLAAIGS LSAFYPDLLN FKEADYELTA IRMIAKIPTI AAMSYKYSIG
     QPFVYPDNSL DFTENFLHMM FATPCTKYKV NPIIKNALNK IFILHADHEQ NASTSTVRIA
     GSSGANAFAC ISTGIASLWG PAHGGANEAV INMLKEIGSS ENIPKYIAKA KDKSDPFRLM
     GFGHRVYKNY DPRASVLKET CKEVLKELGQ LENNPLLQIA IELEAIALKD EYFIERKLYP
     NVDFYSGIIY KAMGIPSQMF TVLFAIARTV GWMAQWKEMH EDPGQKISRP R