ID   CISY_RICAU              Reviewed;         411 AA.
AC   P51039; Q59731;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
DE   Flags: Fragment;
GN   Name=gltA;
OS   Rickettsia australis.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=787;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Phillips;
RX   PubMed=9103608; DOI=10.1099/00207713-47-2-252;
RA   Roux V., Rydkina E., Eremeeva M., Raoult D.;
RT   "Citrate synthase gene comparison, a new tool for phylogenetic analysis,
RT   and its application for the rickettsiae.";
RL   Int. J. Syst. Bacteriol. 47:252-261(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 262-383.
RC   STRAIN=JC;
RA   Higgins J.A., Radulovic S., Schriefer M.E.;
RL   Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; U59718; AAB02954.1; -; Genomic_DNA.
DR   EMBL; U33923; AAB49576.1; -; Genomic_DNA.
DR   AlphaFoldDB; P51039; -.
DR   SMR; P51039; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           <1..>411
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169958"
FT   ACT_SITE        304
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        363
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   CONFLICT        290
FT                   /note="A -> V (in Ref. 2; AAB49576)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="V -> A (in Ref. 2; AAB49576)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        364..365
FT                   /note="FY -> SH (in Ref. 2; AAB49576)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        380
FT                   /note="F -> S (in Ref. 2; AAB49576)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT   NON_TER         411
SQ   SEQUENCE   411 AA;  46112 MW;  9D0571AFD3B6B27D CRC64;
     DSEFAELKIR GKIFKLPILK ASIGEDVIDI SRVSAEADCF TYDPGFMSTA SCQSTITYID
     GDKGILRHRG YNIKDLAEKS DFLEVAYLLI YGELPTIEQY NNFTTQVAHH SLVNERLHYL
     FQAFCSSSHP MAIMLAAVGS LSAFYPDLLN FKEADYELTA IRMIAKIPTI AAMSYKYSIG
     QPFIYPDNSL DFTENFLHMM FATPCTKYKV NPIIKNALNK IFILHADHEQ NASTSTVRIA
     GSSGANAFAC ISTGIASLWG PAHGGANEAV INMLKEIGSS ENIPKFIAKA KDKNDPFRLM
     GFGHRVYKNY DPRAAVLKET CKAVLKELGQ LENNPLLQIA IELEAIALKD EYFIERKLYP
     NVDFYSGIIY KAMGIPSQMF TVLFAIARTV GWMAQWKEMH EDPEQKISRP R