ID   CISY_RICCN              Reviewed;         435 AA.
AC   P51042; Q59737;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2001, sequence version 3.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=RC1308;
OS   Rickettsia conorii (strain ATCC VR-613 / Malish 7).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=272944;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-613 / Malish 7;
RX   PubMed=11557893; DOI=10.1126/science.1061471;
RA   Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V.,
RA   Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.;
RT   "Mechanisms of evolution in Rickettsia conorii and R. prowazekii.";
RL   Science 293:2093-2098(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-418.
RC   STRAIN=ATCC VR-613 / Malish 7;
RX   PubMed=9103608; DOI=10.1099/00207713-47-2-252;
RA   Roux V., Rydkina E., Eremeeva M., Raoult D.;
RT   "Citrate synthase gene comparison, a new tool for phylogenetic analysis,
RT   and its application for the rickettsiae.";
RL   Int. J. Syst. Bacteriol. 47:252-261(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 269-387.
RC   STRAIN=ATCC VR-613 / Malish 7;
RA   Balayeva N., Eremeeva M., Tissot-Dupont H., Zakharov I., Raoult D.;
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- ACTIVITY REGULATION: Allosterically inhibited by NADH. {ECO:0000250}.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; AE006914; AAL03846.1; -; Genomic_DNA.
DR   EMBL; U59730; AAB02958.1; -; Genomic_DNA.
DR   EMBL; U20243; AAA85707.1; -; Genomic_DNA.
DR   PIR; D97863; D97863.
DR   RefSeq; WP_010977866.1; NC_003103.1.
DR   AlphaFoldDB; P51042; -.
DR   SMR; P51042; -.
DR   EnsemblBacteria; AAL03846; AAL03846; RC1308.
DR   KEGG; rco:RC1308; -.
DR   PATRIC; fig|272944.4.peg.1501; -.
DR   HOGENOM; CLU_025068_0_0_5; -.
DR   OMA; TVGWCAQ; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000000816; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..435
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169961"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        370
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   CONFLICT        281
FT                   /note="I -> L (in Ref. 3; AAA85707)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   435 AA;  49210 MW;  4CBFF074A1807853 CRC64;
     MTNENNNDSE FAELKIRGKI FKLPILKASI GEDVIDISRV SAEADCFTYD PGFMSTASCQ
     STITYIDGDK GILRHRGYDI KDLAEKSDFL EVAYLLIYGE LPSGEQYNNF TKQVAHHSLV
     NERLHYLFQT FCSSSHPMAI MLAAVGSLSA FYPDLLNFKE ADYELTAIRM IAKIPTIAAM
     SYKYSIGQPF IYPDNSLDFT ENFLHMMFAT PCTKYTVNPI IKNALNKIFI LHADHEQNAS
     TSTVRIAGSS GANPFACIST GIASLWGPAH GGANEAVINM IKEIGSSEYI PKYIAKAKDK
     NDPFRLMGFG HRVYKNYDPR AAVLKETCKE VLKELGQLDN NPFLQIAIEL EAIALKDEYF
     IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FAIARTVGWM AQWKEMHEDP EQKISRPRQL
     YTGYVHREYK GIRER