ID   CISY_RICFE              Reviewed;         435 AA.
AC   P51040; Q4UJV0;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 2.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=RF_1338;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 279-384.
RX   PubMed=8904435; DOI=10.1128/jcm.34.3.671-674.1996;
RA   Higgins J.A., Radulovic S., Schriefer M.E., Azad A.F.;
RT   "Rickettsia felis: a new species of pathogenic rickettsia isolated from cat
RT   fleas.";
RL   J. Clin. Microbiol. 34:671-674(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; CP000053; AAY62189.1; -; Genomic_DNA.
DR   EMBL; U33922; AAB71724.1; -; Genomic_DNA.
DR   RefSeq; WP_011271638.1; NC_007109.1.
DR   AlphaFoldDB; P51040; -.
DR   SMR; P51040; -.
DR   STRING; 315456.RF_1338; -.
DR   EnsemblBacteria; AAY62189; AAY62189; RF_1338.
DR   KEGG; rfe:RF_1338; -.
DR   eggNOG; COG0372; Bacteria.
DR   HOGENOM; CLU_025068_0_0_5; -.
DR   OMA; TVGWCAQ; -.
DR   OrthoDB; 1412360at2; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..435
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169962"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        370
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   435 AA;  49100 MW;  361CAB47B3274378 CRC64;
     MTNENSNDSE FAELKIRGKI FKLPILKASI GEDVIDISRV SSEADCFTYD PGFMSTASCQ
     STITYIDGDK GILRHRGYDI KDLAEKSDFL EVAYLLIYGE LPSIEQYNNF TKQVAHHSLV
     NERLHYLFQT FCSSSHPMAI MLAAVGSLSA FYPDLLNFKE ADYELTAIRM IAKIPTIAAM
     SYKYSIGQPF IYPDNSLDFT ENFLHMMFAT PCTKYKVNPI IKNALNKIFI LHADHEQNAS
     TSTVRIAGSS GANPFACVST GIASLWGPAH GGANEAVINM LKEIGSSENI PKYIAKAKDK
     NDPFRLMGFG HRVYKNYDPR AAVLKETCKE VLKELGQLEN NPLLQIAIEL EAIALKDEYF
     IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FAIARTVGWM AQWKEMHEDP EQKISRPRQL
     YTGYVHREYK GIGER