ID   CISY_RICPR              Reviewed;         436 AA.
AC   P09948;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 129.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=RP844;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=3112124; DOI=10.1128/jb.169.8.3564-3572.1987;
RA   Wood D.O., Williamson L.R., Winkler H.H., Krause D.C.;
RT   "Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene.";
RL   J. Bacteriol. 169:3564-3572(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
RC   STRAIN=Madrid E;
RX   PubMed=7557459; DOI=10.1016/0378-1119(95)00365-d;
RA   Cai J., Pang H., Wood D.O., Winkler H.H.;
RT   "The citrate synthase-encoding gene of Rickettsia prowazekii is controlled
RT   by two promoters.";
RL   Gene 163:115-119(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 267-392.
RC   STRAIN=ATCC VR-142 / Breinl;
RA   Balayeva N., Eremeeva M., Tissot-Dupont H., Zakharov I., Raoult D.;
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- ACTIVITY REGULATION: Allosterically inhibited by NADH.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; M17149; AAA26383.1; -; Genomic_DNA.
DR   EMBL; AJ235273; CAA15268.1; -; Genomic_DNA.
DR   EMBL; U14334; AAA85524.1; -; Genomic_DNA.
DR   EMBL; U20244; AAA85708.1; -; Genomic_DNA.
DR   PIR; A26971; YKRECP.
DR   RefSeq; NP_221192.1; NC_000963.1.
DR   RefSeq; WP_004596795.1; NC_000963.1.
DR   AlphaFoldDB; P09948; -.
DR   SMR; P09948; -.
DR   STRING; 272947.RP844; -.
DR   EnsemblBacteria; CAA15268; CAA15268; CAA15268.
DR   KEGG; rpr:RP844; -.
DR   PATRIC; fig|272947.5.peg.882; -.
DR   eggNOG; COG0372; Bacteria.
DR   HOGENOM; CLU_025068_0_0_5; -.
DR   OMA; TVGWCAQ; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..436
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169967"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        370
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   436 AA;  49324 MW;  57F947C151A494B8 CRC64;
     MTNGNNNNLE FAELKIRGKL FKLPILKASI GKDVIDISRV SAEADYFTYD PGFMSTASCQ
     STITYIDGDK GILWYRGYDI KDLAEKSDFL EVAYLMIYGE LPSSDQYCNF TKKVAHHSLV
     NERLHYLFQT FCSSSHPMAI MLAAVGSLSA FYPDLLNFNE TDYELTAIRM IAKIPTIAAM
     SYKYSIGQPF IYPDNSLDFT ENFLHMMFAT PCTKYKVNPI IKNALNKIFI LHADHEQNAS
     TSTVRIAGSS GANPFACIST GIASLWGPAH GGANEAVINM LKEIGSSENI PKYVAKAKDK
     NDPFRLMGFG HRVYKSYDPR AAVLKETCKE VLNELGQLDN NPLLQIAIEL EALALKDEYF
     IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FAIARTVGWM AQWKEMHEDP EQKISRPRQL
     YTGYVHREYK CIVERK