ID   CISY_RICS2              Reviewed;         435 AA.
AC   Q59778; Q7PA47;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   12-SEP-2018, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; ORFNames=rsib_orf788 {ECO:0000312|EMBL:EAA25990.1};
OS   Rickettsia sibirica (strain ATCC VR-151 / 246).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group;
OC   Rickettsia sibirica subgroup.
OX   NCBI_TaxID=272951;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-151 / 246;
RA   Malek J.A., Eremeeva M.E., Dasch G.A.;
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-418.
RC   STRAIN=ATCC VR-151 / 246;
RX   PubMed=9103608; DOI=10.1099/00207713-47-2-252;
RA   Roux V., Rydkina E., Eremeeva M., Raoult D.;
RT   "Citrate synthase gene comparison, a new tool for phylogenetic analysis,
RT   and its application for the rickettsiae.";
RL   Int. J. Syst. Bacteriol. 47:252-261(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; AABW01000001; EAA25990.1; -; Genomic_DNA.
DR   EMBL; U59734; AAB02971.1; -; Genomic_DNA.
DR   RefSeq; WP_004997172.1; NZ_AABW01000001.1.
DR   AlphaFoldDB; Q59778; -.
DR   SMR; Q59778; -.
DR   STRING; 272951.rsib_orf788; -.
DR   EnsemblBacteria; EAA25990; EAA25990; rsib_orf788.
DR   eggNOG; COG0372; Bacteria.
DR   HOGENOM; CLU_025068_0_0_5; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000004455; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..435
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169969"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        370
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   435 AA;  49176 MW;  AB5682B38B0A27F3 CRC64;
     MTNENNNDSE FAELKIRGKI FKLPILKASI GEDVIDISRV SAEADCFTYD PGFMSTASCQ
     STITYIDGDK GILRHRGYDI KDLAEKSDFL EVAYLLIYGE LPSGEQYNNF TKQVAHHSLV
     NERLHYLFQT FCSSSHPMAI MLAAVGSLSA FYPDLLNFKE ADYELTAIRM IAKIPTIAAM
     SYKYSIGQPF IYPDNSLDFT ENFLHMMFAT PCTKYTVNPI IKNALNKIFI LHADHEQNAS
     TSTVRIAGSS GANPFACIST GIASLWGPAH GGANEAVINM LKEIGSSEYI PKYIAKAKDK
     NDPFRLMGFG HRVYKNYDPR AAVLKETCKE VLKELGQLDN NPLLQIAIEL EAIALKDEYF
     IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FAIARTVGWM AQWKEMHEDP EQKISRPRQL
     YTGYVHREYK GIRER