ID   CISY_SALTY              Reviewed;         427 AA.
AC   O68883;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=STM0730;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RA   Horswill A.R., Dudding A.R., Escalante-Semerena J.C.;
RT   "Complete sequence of the citrate synthase gene of Salmonella typhimurium
RT   LT2.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- ACTIVITY REGULATION: Allosterically inhibited by NADH. {ECO:0000250}.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; AF056043; AAC12779.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL19674.1; -; Genomic_DNA.
DR   RefSeq; NP_459715.1; NC_003197.2.
DR   RefSeq; WP_000785817.1; NC_003197.2.
DR   AlphaFoldDB; O68883; -.
DR   SMR; O68883; -.
DR   STRING; 99287.STM0730; -.
DR   PaxDb; O68883; -.
DR   PRIDE; O68883; -.
DR   EnsemblBacteria; AAL19674; AAL19674; STM0730.
DR   GeneID; 1252250; -.
DR   KEGG; stm:STM0730; -.
DR   PATRIC; fig|99287.12.peg.762; -.
DR   HOGENOM; CLU_025068_0_0_6; -.
DR   PhylomeDB; O68883; -.
DR   BioCyc; SENT99287:STM0730-MON; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..427
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169945"
FT   ACT_SITE        306
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        363
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   427 AA;  48106 MW;  2E708DBF21FAE1CB CRC64;
     MADTKAKITL TGDTTIELDV LKGTLGQDVI DIRSLGSKGV FTFDPGFTST ASCESKITFI
     DGDEGILLHR GFPIDQLATD SNYLEVCYIL LYGEKPTQEE YDEFRTTVTR HTMIHEQITR
     LFHAFRRDSH PMAVMCGITG ALAAFYHDSL DVNNPRHREI AAFRLLSKMP TMAAMCYKYS
     IGQPFVYPRN DLSYAGNFLN MMFSTPCETY EVNPVLERAM DRILILHADH EQNASTSTVR
     TAGSSGANPF ACIAAGIASL WGPAHGGANE AALKMLEEIS SVKHIPEFVR RAKDKNDSFR
     LMGFGHRVYK NYDPRATVMR ETCHEVLKEL GTKDDLLEVA MELEHIALND PYFIEKKLYP
     NVDFYSGIIL KAMGIPSSMF TVIFAMARTV GWIAHWNEMH TDGMKIARPR QLYTGYDKRD
     FKSALKR