ID   CISY_STRHY              Reviewed;          20 AA.
AC   P20903;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
DE   Flags: Fragment;
GN   Name=gltA;
OS   Streptomyces hygroscopicus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces violaceusniger group.
OX   NCBI_TaxID=1912;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 21705 / DSM 41527 / SF-1293;
RX   PubMed=1368511; DOI=10.1271/bbb1961.54.463;
RA   Shimotohno K.W., Imai S., Murakami T., Seto H.;
RT   "Purification and characterization of citrate synthase from Streptomyces
RT   hygroscopicus SF-1293 and comparison of its properties with those of 2-
RT   phosphinomethylmalic acid synthase.";
RL   Agric. Biol. Chem. 54:463-470(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- ACTIVITY REGULATION: Allosterically inhibited by NADH.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   PIR; PQ0046; PQ0046.
DR   AlphaFoldDB; P20903; -.
DR   STRING; 68042.GCA_001553435_06284; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   Allosteric enzyme; Direct protein sequencing; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..>20
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169972"
FT   NON_TER         20
SQ   SEQUENCE   20 AA;  2234 MW;  C527EC7A87119597 CRC64;
     SDNSVVLRYG DGEYSYPVVD