ID   CISY_WEICA              Reviewed;         373 AA.
AC   P27660;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=ctsA;
OS   Weizmannia coagulans (Bacillus coagulans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Weizmannia.
OX   NCBI_TaxID=1398;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 55182;
RX   PubMed=1311544; DOI=10.1128/aem.58.1.335-345.1992;
RA   Schendel F.J., August P.R., Anderson C.R., Hanson R.S., Flickinger M.C.;
RT   "Cloning and nucleotide sequence of the gene coding for citrate synthase
RT   from a thermotolerant Bacillus sp.";
RL   Appl. Environ. Microbiol. 58:335-345(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; M74818; AAA52231.1; -; Genomic_DNA.
DR   AlphaFoldDB; P27660; -.
DR   SMR; P27660; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   PANTHER; PTHR11739; PTHR11739; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..373
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169927"
FT   ACT_SITE        262
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        314
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   373 AA;  41970 MW;  B4B6FC7170077523 CRC64;
     MVNTNQFIPG LEGVIASETK ISFLDTVNSE IVIKGYDLLA LSKTKGYLDI VHLLLEGTIP
     NEAEKQHLEE TLKQEYDVPD EIIQVLSLLP KTAHPMDALR TGVSVLASFD TELLNREHST
     NLKRAYQLLG KIPNIVANSY HILHSEEPVQ PLQDLSYSAN FLYMITGKKP TELEEKIFDR
     SLVLYSEHEL PNSTFTARVI ASTLSDLYGA LTGAVASLKG HLHGGANEAV MEMLQDAQTV
     EGFKHLLHDK LSKKEKIMGF GHRVYMKKMD PRAAMMKEAL KELSAVNGDD LLLQMCEAGE
     QIMREEKGLF PNLDYYAAPV YWKLGIPIPL YTPIFFSSRT VGLCAHVMEQ HENNRIFRPR
     VLYTGARNLR VED