CITA_ECOLX
ID CITA_ECOLX Reviewed; 431 AA.
AC P07661; P07680;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Citrate-proton symporter;
DE AltName: Full=Citrate carrier protein;
DE AltName: Full=Citrate transporter;
DE AltName: Full=Citrate utilization determinant;
DE AltName: Full=Citrate utilization protein A;
GN Name=citA; Synonyms=cit;
OS Escherichia coli.
OG Plasmid pWR60, and Plasmid pOH3001.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pWR60;
RX PubMed=2999088; DOI=10.1128/jb.164.3.983-993.1985;
RA Sasatsu M., Misra T.K., Chu L., Laddaga R., Silver S.;
RT "Cloning and DNA sequence of a plasmid-determined citrate utilization
RT system in Escherichia coli.";
RL J. Bacteriol. 164:983-993(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pOH3001; TRANSPOSON=Tn3411;
RX PubMed=2999087; DOI=10.1128/jb.164.3.977-982.1985;
RA Ishiguro N., Sato G.;
RT "Nucleotide sequence of the gene determining plasmid-mediated citrate
RT utilization.";
RL J. Bacteriol. 164:977-982(1985).
CC -!- FUNCTION: Uptake of citrate across the boundary membrane with the
CC concomitant transport of protons into the cell (symport system).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- MISCELLANEOUS: Allows the utilization of citrate as a sole source of
CC carbon and energy.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR EMBL; M11559; AAA88472.1; -; Genomic_DNA.
DR EMBL; M22041; AAA98398.1; -; Genomic_DNA.
DR PIR; A23103; ZTEC3.
DR PIR; B23104; ZTEC6.
DR RefSeq; WP_050153616.1; NZ_SPXL01000106.1.
DR AlphaFoldDB; P07661; -.
DR SMR; P07661; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006101; P:citrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004736; MHS_symport.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00883; 2A0106; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 2.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Citrate utilization; Membrane; Plasmid;
KW Symport; Transmembrane; Transmembrane helix; Transport;
KW Transposable element.
FT CHAIN 1..431
FT /note="Citrate-proton symporter"
FT /id="PRO_0000050298"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..51
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..108
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..273
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..301
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..332
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 354..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 385..397
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..431
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 88
FT /note="I -> V (in transposon Tn3411)"
FT VARIANT 316
FT /note="L -> W (in transposon Tn3411)"
FT VARIANT 414
FT /note="T -> A (in transposon Tn3411)"
FT VARIANT 418
FT /note="A -> V (in transposon Tn3411)"
FT VARIANT 422
FT /note="S -> R (in transposon Tn3411)"
FT VARIANT 427
FT /note="V -> A (in transposon Tn3411)"
SQ SEQUENCE 431 AA; 46980 MW; C2D55DC6B53D2CFE CRC64;
MTQQPSRAGT FGAILRVTSG NFLEQFDFFL FGFYATYIAK TFFPAESEFA ALMLTFAVFG
SGFLMRPIGA VVLGAYIDRI GRRKGLMITL AIMGCGTLLI ALVPGYQTIG LLAPVLVLVG
RLLQGFSAGV ELGGVSVYLS EIATPGNKGF YTSWQSASQQ VAIVVAALIG YGLNVTLGHD
EISEWGWRIP FFIGCMIIPL IFVLRRSLQE TEAFLQRKHR PDTREIFTTI AKNWRIITAG
TLLVAMTTTT FYFITVYTPT YGRTVLNLSA RDSLVVTMLV GISNFIWLPI GGAISDRIGR
RPVLMGITLL ALVTTLPVMN WLTAAPDFTR MTLVLLWFSF FFGMYNGAMV AALTEVMPVY
VRTVGFSLAF SLATAIFGGL TPAISTALVQ LTGDKSSPGW WLMCAALCGL AATTMLFARL
SSGYQTVENK L
