ACHA4_CHICK
ID ACHA4_CHICK Reviewed; 622 AA.
AC P09482;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Neuronal acetylcholine receptor subunit alpha-4;
DE Flags: Precursor;
GN Name=CHRNA4;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=White leghorn; TISSUE=Brain;
RX PubMed=3267226; DOI=10.1002/j.1460-2075.1988.tb02852.x;
RA Nef P., Oneyser C., Alliod C., Couturier S., Ballivet M.;
RT "Genes expressed in the brain define three distinct neuronal nicotinic
RT acetylcholine receptors.";
RL EMBO J. 7:595-601(1988).
RN [2]
RP MUTAGENESIS OF GLU-289, AND SUBUNIT.
RX PubMed=2005979; DOI=10.1038/350235a0;
RA Cooper E., Couturier S., Ballivet M.;
RT "Pentameric structure and subunit stoichiometry of a neuronal nicotinic
RT acetylcholine receptor.";
RL Nature 350:235-238(1991).
CC -!- FUNCTION: After binding acetylcholine, the AChR responds by an
CC extensive change in conformation that affects all subunits and leads to
CC opening of an ion-conducting channel across the plasma membrane.
CC -!- SUBUNIT: Neuronal AChR seems to be composed of two different type of
CC subunits: alpha and non-alpha (also called beta). A functional receptor
CC seems to consist of two alpha-chains and three non-alpha chains.
CC {ECO:0000269|PubMed:2005979}.
CC -!- INTERACTION:
CC P09482; P09484: CHRNB2; NbExp=7; IntAct=EBI-10686088, EBI-10686072;
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein. Cell membrane
CC {ECO:0000250}; Lipid-anchor {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; X07348; CAA30285.1; -; Genomic_DNA.
DR EMBL; X07349; CAA30285.1; JOINED; Genomic_DNA.
DR EMBL; X07350; CAA30285.1; JOINED; Genomic_DNA.
DR EMBL; X07351; CAA30285.1; JOINED; Genomic_DNA.
DR EMBL; X07352; CAA30285.1; JOINED; Genomic_DNA.
DR EMBL; X07399; CAA30285.1; JOINED; Genomic_DNA.
DR EMBL; AJ250361; CAB59626.1; -; mRNA.
DR PIR; S00379; ACCH4N.
DR RefSeq; NP_990145.1; NM_204814.1.
DR AlphaFoldDB; P09482; -.
DR SMR; P09482; -.
DR ComplexPortal; CPX-172; Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2.
DR ComplexPortal; CPX-173; Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2.
DR ComplexPortal; CPX-217; Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2.
DR ComplexPortal; CPX-221; Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4.
DR IntAct; P09482; 1.
DR STRING; 9031.ENSGALP00000009302; -.
DR BindingDB; P09482; -.
DR ChEMBL; CHEMBL5569; -.
DR DrugCentral; P09482; -.
DR PaxDb; P09482; -.
DR Ensembl; ENSGALT00000009316; ENSGALP00000009302; ENSGALG00000005801.
DR GeneID; 395606; -.
DR KEGG; gga:395606; -.
DR CTD; 1137; -.
DR VEuPathDB; HostDB:geneid_395606; -.
DR eggNOG; KOG3645; Eukaryota.
DR GeneTree; ENSGT00940000159329; -.
DR HOGENOM; CLU_018074_1_1_1; -.
DR InParanoid; P09482; -.
DR OMA; IFPAFGH; -.
DR OrthoDB; 381858at2759; -.
DR PhylomeDB; P09482; -.
DR TreeFam; TF315605; -.
DR PRO; PR:P09482; -.
DR Proteomes; UP000000539; Chromosome 20.
DR Bgee; ENSGALG00000005801; Expressed in brain and 3 other tissues.
DR ExpressionAtlas; P09482; baseline and differential.
DR GO; GO:0005892; C:acetylcholine-gated channel complex; IPI:ComplexPortal.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0022848; F:acetylcholine-gated cation-selective channel activity; IBA:GO_Central.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051899; P:membrane depolarization; IDA:ComplexPortal.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0035094; P:response to nicotine; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0007271; P:synaptic transmission, cholinergic; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 2.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00254; NICOTINICR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Lipoprotein; Membrane; Palmitate;
KW Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..23
FT CHAIN 24..622
FT /note="Neuronal acetylcholine receptor subunit alpha-4"
FT /id="PRO_0000000355"
FT TOPO_DOM 24..237
FT /note="Extracellular"
FT TRANSMEM 238..262
FT /note="Helical"
FT TRANSMEM 270..288
FT /note="Helical"
FT TRANSMEM 304..325
FT /note="Helical"
FT TOPO_DOM 326..595
FT /note="Cytoplasmic"
FT TRANSMEM 596..614
FT /note="Helical"
FT REGION 380..477
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 497..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..407
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 429..476
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..513
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 266
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 156..170
FT /evidence="ECO:0000250"
FT DISULFID 220..221
FT /note="Associated with receptor activation"
FT /evidence="ECO:0000250"
FT MUTAGEN 289
FT /note="E->K: Reduces channel conductance by 50%."
FT /evidence="ECO:0000269|PubMed:2005979"
SQ SEQUENCE 622 AA; 70838 MW; 290B035893D6485A CRC64;
MGFLVSKGNL LLLLCASIFP AFGHVETRAH AEERLLKKLF SGYNKWSRPV ANISDVVLVR
FGLSIAQLID VDEKNQMMTT NVWVKQEWHD YKLRWDPQEY ENVTSIRIPS ELIWRPDIVL
YNNADGDFAV THLTKAHLFY DGRIKWMPPA IYKSSCSIDV TFFPFDQQNC KMKFGSWTYD
KAKIDLVSMH SHVDQLDYWE SGEWVIINAV GNYNSKKYEC CTEIYPDITY SFIIRRLPLF
YTINLIIPCL LISCLTVLVF YLPSECGEKI TLCISVLLSL TVFLLLITEI IPSTSLVIPL
IGEYLLFTMI FVTLSIIITV FVLNVHHRSP RTHTMPDWVR RVFLDIVPRL LFMKRPSTVK
DNCKKLIESM HKLTNSPRLW SETDMEPNFT TSSSPSPQSN EPSPTSSFCA HLEEPAKPMC
KSPSGQYSML HPEPPQVTCS SPKPSCHPLS DTQTTSISKG RSLSVQQMYS PNKTEEGSIR
CRSRSIQYCY LQEDSSQTNG HSSASPASQR CHLNEEQPQH KPHQCKCKCR KGEAAGTPTQ
GSKSHSNKGE HLVLMSPALK LAVEGVHYIA DHLRAEDADF SVKEDWKYVA MVIDRIFLWM
FIIVCLLGTV GLFLPPWLAG MI
