CITM_BACSU
ID CITM_BACSU Reviewed; 433 AA.
AC P55069;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Mg(2+)/citrate complex secondary transporter;
GN Name=citM; Synonyms=yflO; OrderedLocusNames=BSU07610;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / 6GM;
RX PubMed=8892821; DOI=10.1128/jb.178.21.6216-6222.1996;
RA Boorsma A., van der Rest M.E., Lolkema J.S., Konings W.N.;
RT "Secondary transporters for citrate and the Mg(2+)-citrate complex in
RT Bacillus subtilis are homologous proteins.";
RL J. Bacteriol. 178:6216-6222(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / AC327;
RX PubMed=9272861; DOI=10.1016/s0378-1119(97)00130-3;
RA Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J.;
RT "Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of
RT the Bacillus subtilis genome reveal genes for a new two-component system,
RT three spore germination proteins, an iron uptake system and a general
RT stress response protein.";
RL Gene 194:191-199(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP TRANSCRIPTIONAL REGULATION.
RC STRAIN=168;
RX PubMed=11029430; DOI=10.1128/jb.182.21.6099-6105.2000;
RA Warner J.B., Krom B.P., Magni C., Konings W.N., Lolkema J.S.;
RT "Catabolite repression and induction of the Mg(2+)-citrate transporter CitM
RT of Bacillus subtilis.";
RL J. Bacteriol. 182:6099-6105(2000).
RN [5]
RP TRANSCRIPTIONAL REGULATION.
RC STRAIN=168;
RX PubMed=10972810; DOI=10.1046/j.1365-2958.2000.02055.x;
RA Yamamoto H., Murata M., Sekiguchi J.;
RT "The CitST two-component system regulates the expression of the Mg-citrate
RT transporter in Bacillus subtilis.";
RL Mol. Microbiol. 37:898-912(2000).
RN [6]
RP CHARACTERIZATION.
RC STRAIN=168;
RX PubMed=11053381; DOI=10.1128/jb.182.22.6374-6381.2000;
RA Krom B.P., Warner J.B., Konings W.N., Lolkema J.S.;
RT "Complementary metal ion specificity of the metal-citrate transporters CitM
RT and CitH of Bacillus subtilis.";
RL J. Bacteriol. 182:6374-6381(2000).
RN [7]
RP CHARACTERIZATION.
RC STRAIN=168;
RX PubMed=11891560; DOI=10.1007/s00232-001-0106-1;
RA Li H., Pajor A.M.;
RT "Functional characterization of CitM, the Mg2+-citrate transporter.";
RL J. Membr. Biol. 185:9-16(2002).
CC -!- FUNCTION: Proton motive force-driven secondary transporter that
CC mediates the transport of citrate complexed to Mg(2+). Cotransports at
CC least two protons per Mg(2+)-citrate complex. Can also transport
CC citrate in complex with Ni(2+), Mn(2+), Co(2+), and Zn(2+).
CC -!- ACTIVITY REGULATION: The uptake activity increases with increasing
CC Mg(2+) concentrations. Inhibited by FCCP, TCC and nigericin.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Expression is under strict control of the medium
CC composition. Induced by citrate, via the two-component regulatory
CC system CitT/CitS. Repressed by rapidly metabolized carbon sources like
CC glucose, glycerol and inositol, via the carbon catabolite repression
CC system. Is also repressed by succinate and glutamate, albeit to a
CC lesser extent. {ECO:0000269|PubMed:10972810,
CC ECO:0000269|PubMed:11029430}.
CC -!- SIMILARITY: Belongs to the CitM (TC 2.A.11) transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA22308.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U62003; AAC44564.1; -; Genomic_DNA.
DR EMBL; D86417; BAA22308.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL009126; CAB12590.1; -; Genomic_DNA.
DR PIR; C69600; C69600.
DR RefSeq; NP_388642.1; NC_000964.3.
DR RefSeq; WP_003242978.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; P55069; -.
DR STRING; 224308.BSU07610; -.
DR TCDB; 2.A.11.1.1; the citrate-mg(2+):h(+) (citm) citrate-ca(2+):h(+) (cith) symporter (citmhs) family.
DR PaxDb; P55069; -.
DR PRIDE; P55069; -.
DR EnsemblBacteria; CAB12590; CAB12590; BSU_07610.
DR GeneID; 938801; -.
DR KEGG; bsu:BSU07610; -.
DR PATRIC; fig|224308.179.peg.827; -.
DR eggNOG; COG2851; Bacteria.
DR InParanoid; P55069; -.
DR OMA; VMNILPW; -.
DR PhylomeDB; P55069; -.
DR BioCyc; BSUB:BSU07610-MON; -.
DR PRO; PR:P55069; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0098656; P:anion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006101; P:citrate metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR InterPro; IPR014738; Citrate_transporter.
DR Pfam; PF03600; CitMHS; 1.
DR TIGRFAMs; TIGR00784; citMHS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Citrate utilization; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..433
FT /note="Mg(2+)/citrate complex secondary transporter"
FT /id="PRO_0000089774"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 433 AA; 45830 MW; 7ECC4DDA835DBE05 CRC64;
MLAILGFLMM LVFMALIMTK RLSVLTALVL TPIVFALIAG FGFTEVGDMM ISGIQQVAPT
AVMIMFAILY FGIMIDTGLF DPMVGKILSM VKGDPLKIVV GTAVLTMLVA LDGDGSTTYM
ITTSAMLPLY LLLGIRPIIL AGIAGVGMGI MNTIPWGGAT PRAASALGVD PAELTGPMIP
VIASGMLCMV AVAYVLGKAE RKRLGVIELK QPANANEPAA AVEDEWKRPK LWWFNLLLTL
SLIGCLVSGK VSLTVLFVIA FCIALIVNYP NLEHQRQRIA AHSSNVLAIG SMIFAAGVFT
GILTGTKMVD EMAISLVSMI PEQMGGLIPA IVALTSGIFT FLMPNDAYFY GVLPILSETA
VAYGVDKVEI ARASIIGQPI HMLSPLVPST HLLVGLVGVS IDDHQKFALK WAVLAVIVMT
AIALLIGAIS ISV
