CITN_KLEPN
ID CITN_KLEPN Reviewed; 446 AA.
AC P31602;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Citrate-sodium symporter;
DE AltName: Full=Citrate carrier protein;
DE AltName: Full=Citrate transporter;
GN Name=citS;
OS Klebsiella pneumoniae.
OG Plasmid pES3.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13882 / NBRC 13541 / NCTC 8172;
RX PubMed=1577734; DOI=10.1016/s0021-9258(19)50375-1;
RA van der Rest M.E., Siewe R.M., Abee T., Schwarz E., Oesterhelt D.,
RA Konings W.N.;
RT "Nucleotide sequence and functional properties of a sodium-dependent
RT citrate transport system from Klebsiella pneumoniae.";
RL J. Biol. Chem. 267:8971-8976(1992).
RN [2]
RP TOPOLOGY.
RX PubMed=8810332; DOI=10.1074/jbc.271.41.25582;
RA van Geest M., Lolkema J.S.;
RT "Membrane topology of the sodium ion-dependent citrate carrier of
RT Klebsiella pneumoniae. Evidence for a new structural class of secondary
RT transporters.";
RL J. Biol. Chem. 271:25582-25589(1996).
CC -!- FUNCTION: Uptake of citrate across the boundary membrane with the
CC concomitant uptake of a sodium ion (symport system).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- MISCELLANEOUS: Allows the utilization of citrate as a sole source of
CC carbon and energy.
CC -!- SIMILARITY: Belongs to the sodium:citrate (SCF) symporter family.
CC {ECO:0000305}.
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DR EMBL; M83146; AAA25060.1; -; Genomic_DNA.
DR PIR; A38244; A38244.
DR PDB; 5X9R; X-ray; 3.98 A; A/B=13-446.
DR PDB; 5XAR; X-ray; 3.62 A; A/B/C/D=13-446.
DR PDB; 5XAS; X-ray; 3.47 A; A/B=13-446.
DR PDB; 5XAT; X-ray; 3.76 A; A/B/C/D=13-446.
DR PDBsum; 5X9R; -.
DR PDBsum; 5XAR; -.
DR PDBsum; 5XAS; -.
DR PDBsum; 5XAT; -.
DR AlphaFoldDB; P31602; -.
DR SMR; P31602; -.
DR DIP; DIP-60559N; -.
DR TCDB; 2.A.24.1.1; the 2-hydroxycarboxylate transporter (2-hct) family.
DR ABCD; P31602; 1 sequenced antibody.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006101; P:citrate metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR018025; 2-OHcarbox_trans_Prot/Firm.
DR InterPro; IPR004679; 2-OHcarboxylate_transport.
DR PANTHER; PTHR40033; PTHR40033; 1.
DR Pfam; PF03390; 2HCT; 1.
DR PIRSF; PIRSF005348; YxkH; 1.
DR TIGRFAMs; TIGR00783; ccs; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Citrate utilization; Membrane; Plasmid;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..446
FT /note="Citrate-sodium symporter"
FT /id="PRO_0000088755"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 23..43
FT /note="Helical; Name=I"
FT /evidence="ECO:0000305"
FT TOPO_DOM 44..45
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 46..66
FT /note="Helical; Name=II"
FT /evidence="ECO:0000305"
FT TOPO_DOM 67..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 79..99
FT /note="Helical; Name=III"
FT /evidence="ECO:0000305"
FT TOPO_DOM 100..109
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 110..130
FT /note="Helical; Name=IV"
FT /evidence="ECO:0000305"
FT TOPO_DOM 131..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 148..168
FT /note="Helical; Name=V"
FT /evidence="ECO:0000305"
FT TOPO_DOM 169..212
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 213..233
FT /note="Helical; Name=VI"
FT /evidence="ECO:0000305"
FT TOPO_DOM 234..266
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 267..287
FT /note="Helical; Name=VII"
FT /evidence="ECO:0000305"
FT TOPO_DOM 288..360
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 361..379
FT /note="Helical; Name=VIII"
FT /evidence="ECO:0000305"
FT TOPO_DOM 380..426
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8810332"
FT TRANSMEM 427..446
FT /note="Helical; Name=IX"
FT /evidence="ECO:0000305"
FT HELIX 17..20
FT /evidence="ECO:0007829|PDB:5XAS"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 28..43
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 51..71
FT /evidence="ECO:0007829|PDB:5XAS"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 78..81
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 83..95
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 101..111
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 116..131
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 137..165
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 170..176
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 178..182
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 185..188
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 190..200
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 205..236
FT /evidence="ECO:0007829|PDB:5XAS"
FT TURN 238..240
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 265..288
FT /evidence="ECO:0007829|PDB:5XAS"
FT STRAND 293..296
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 300..314
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 319..334
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 337..347
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 351..355
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 360..384
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 388..394
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 397..400
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 403..413
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 417..419
FT /evidence="ECO:0007829|PDB:5XAS"
FT HELIX 420..445
FT /evidence="ECO:0007829|PDB:5XAS"
SQ SEQUENCE 446 AA; 47558 MW; D90AB990BDDDE879 CRC64;
MTNMSQPPAT EKKGVSDLLG FKIFGMPLPL YAFALITLLL SHFYNALPTD IVGGFAIMFI
IGAIFGEIGK RLPIFNKYIG GAPVMIFLVA AYFVYAGIFT QKEIDAISNV MDKSNFLNLF
IAVLITGAIL SVNRRLLLKS LLGYIPTILM GIVGASIFGI AIGLVFGIPV DRIMMLYVLP
IMGGGNGAGA VPLSEIYHSV TGRSREEYYS TAIAILTIAN IFAIVFAAVL DIIGKKHTWL
SGEGELVRKA SFKVEEDEKT GQITHRETAV GLVLSTTCFL LAYVVAKKIL PSIGGVAIHY
FAWMVLIVAA LNASGLCSPE IKAGAKRLSD FFSKQLLWVL MVGVGVCYTD LQEIINAITF
ANVVIAAIIV IGAVLGAAIG GWLMGFFPIE SAITAGLCMA NRGGSGDLEV LSACNRMNLI
SYAQISSRLG GGIVLVIASI VFGMMI
