ID   CITR_BACSU              Reviewed;         291 AA.
AC   P39127;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=HTH-type transcriptional regulator CitR;
DE   AltName: Full=Citrate synthase I repressor;
GN   Name=citR; OrderedLocusNames=BSU09430;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / SMY;
RX   PubMed=8045898; DOI=10.1128/jb.176.15.4669-4679.1994;
RA   Jin S., Sonenshein A.L.;
RT   "Identification of two distinct Bacillus subtilis citrate synthase genes.";
RL   J. Bacteriol. 176:4669-4679(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA   Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA   Venema G., Bron S.;
RT   "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT   subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT   many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL   Microbiology 144:859-875(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SEQUENCE REVISION TO 46-50 AND 268-291.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [5]
RP   FUNCTION.
RX   PubMed=8045899; DOI=10.1128/jb.176.15.4680-4690.1994;
RA   Jin S., Sonenshein A.L.;
RT   "Transcriptional regulation of Bacillus subtilis citrate synthase genes.";
RL   J. Bacteriol. 176:4680-4690(1994).
CC   -!- FUNCTION: Negative regulatory protein for the citA gene for citrate
CC       synthase I. {ECO:0000269|PubMed:8045899}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; U05256; AAA20935.1; -; Genomic_DNA.
DR   EMBL; Y14082; CAA74488.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12782.2; -; Genomic_DNA.
DR   PIR; I40379; I40379.
DR   RefSeq; NP_388824.2; NC_000964.3.
DR   RefSeq; WP_003233357.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P39127; -.
DR   SMR; P39127; -.
DR   STRING; 224308.BSU09430; -.
DR   PaxDb; P39127; -.
DR   PRIDE; P39127; -.
DR   EnsemblBacteria; CAB12782; CAB12782; BSU_09430.
DR   GeneID; 939267; -.
DR   KEGG; bsu:BSU09430; -.
DR   PATRIC; fig|224308.179.peg.1016; -.
DR   eggNOG; COG0583; Bacteria.
DR   InParanoid; P39127; -.
DR   OMA; HPVYWED; -.
DR   PhylomeDB; P39127; -.
DR   BioCyc; BSUB:BSU09430-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..291
FT                   /note="HTH-type transcriptional regulator CitR"
FT                   /id="PRO_0000105610"
FT   DOMAIN          1..58
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        18..37
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   CONFLICT        46..50
FT                   /note="KLFER -> NVFDV (in Ref. 1; AAA20935 and 2;
FT                   CAA74488)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        268..291
FT                   /note="AYAIALYENKKEKKFLDFLSHFHF -> LTRLPFMKIRKKKSSWIFYHIFIF
FT                   RSNQNACAPSNEKNPFQ (in Ref. 1; AAA20935 and 2; CAA74488)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   291 AA;  33667 MW;  F8185FF542368ECD CRC64;
     MDFKWLHTFV TAAKYENFRK TAETLFLSQP TVTVHIKQLE KEISCKLFER KGRQIQLTDE
     GRAYLPYALR LLDDYENSMA ELHRVRQGYS QTLQLAVSPL IADTVLPSVM KRYTAMNTET
     EMAVTIFESA EIASLIKAGE ADIGLSCLKV QSSSLSCHCL YKDPVVLVAP PDKRFIEDNE
     IDAKEVLEQY LLLTHNHPDY WDDLLRQVRM TFPFVRTMKV TQTHITKRFI KEGLGVSFLP
     LSTVKRELAE KQMIRIPYQS VQLPYAGAYA IALYENKKEK KFLDFLSHFH F