ID   CITT_BACSU              Reviewed;         226 AA.
AC   O34534;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Transcriptional regulatory protein CitT;
GN   Name=citT; Synonyms=yflQ; OrderedLocusNames=BSU07590;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / AC327;
RX   PubMed=9272861; DOI=10.1016/s0378-1119(97)00130-3;
RA   Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J.;
RT   "Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of
RT   the Bacillus subtilis genome reveal genes for a new two-component system,
RT   three spore germination proteins, an iron uptake system and a general
RT   stress response protein.";
RL   Gene 194:191-199(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=168;
RX   PubMed=10972810; DOI=10.1046/j.1365-2958.2000.02055.x;
RA   Yamamoto H., Murata M., Sekiguchi J.;
RT   "The CitST two-component system regulates the expression of the Mg-citrate
RT   transporter in Bacillus subtilis.";
RL   Mol. Microbiol. 37:898-912(2000).
RN   [4]
RP   FUNCTION.
RX   PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA   Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA   Fujita Y.;
RT   "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT   regulatory systems.";
RL   J. Bacteriol. 183:7365-7370(2001).
CC   -!- FUNCTION: Member of the two-component regulatory system CitT/CitS.
CC       Regulates the expression of the citM-yflN operon. Phosphorylated CitT
CC       binds to the citM promoter to activate the transcription of the citM-
CC       yflN operon. {ECO:0000269|PubMed:11717295}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by CitS. {ECO:0000250}.
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DR   EMBL; D86417; BAA22310.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12588.1; -; Genomic_DNA.
DR   PIR; F69600; F69600.
DR   RefSeq; NP_388640.1; NC_000964.3.
DR   RefSeq; WP_003242754.1; NZ_JNCM01000032.1.
DR   AlphaFoldDB; O34534; -.
DR   SMR; O34534; -.
DR   IntAct; O34534; 1.
DR   STRING; 224308.BSU07590; -.
DR   PaxDb; O34534; -.
DR   EnsemblBacteria; CAB12588; CAB12588; BSU_07590.
DR   GeneID; 938806; -.
DR   KEGG; bsu:BSU07590; -.
DR   PATRIC; fig|224308.179.peg.824; -.
DR   eggNOG; COG4565; Bacteria.
DR   InParanoid; O34534; -.
DR   OMA; FMVAKLH; -.
DR   PhylomeDB; O34534; -.
DR   BioCyc; BSUB:BSU07590-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR013196; HTH_11.
DR   InterPro; IPR024187; Sig_transdc_resp-reg_cit/mal.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR028141; Transcriptional_reg_dom.
DR   PANTHER; PTHR45526; PTHR45526; 1.
DR   Pfam; PF12431; CitT; 1.
DR   Pfam; PF08279; HTH_11; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PIRSF; PIRSF006171; RR_citrat_malat; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..226
FT                   /note="Transcriptional regulatory protein CitT"
FT                   /id="PRO_0000081074"
FT   DOMAIN          3..119
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        178..197
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         54
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   226 AA;  25410 MW;  55FA395A08A38BD3 CRC64;
     MIHIAIAEDD FRVAQIHERL IKQLDGFKII GKAANAKETL ALLKEHKADL LLLDIYMPDE
     LGTALIPDIR SRFPEVDIMI ITAATETRHL QEALRAGIAH YLIKPVTADK FRQVLLQYKE
     KRKLLMSQPE VSQSMIDHIF GNGVKTALPA EDLPTGINSI TLRKIKEALQ TASEGLTAEE
     LGEKMGASRT TARRYAEYLV SKEEARAELE YGIIGRPERK YYLAAD