CITT_ECOLI
ID CITT_ECOLI Reviewed; 487 AA.
AC P0AE74; P77405;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Citrate/succinate antiporter;
DE AltName: Full=Citrate carrier;
DE AltName: Full=Citrate transporter;
GN Name=citT; Synonyms=ybdS; OrderedLocusNames=b0612, JW0604;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION.
RX PubMed=9696764; DOI=10.1128/jb.180.16.4160-4165.1998;
RA Pos K.M., Dimroth P., Bott M.;
RT "The Escherichia coli citrate carrier CitT: a member of a novel eubacterial
RT transporter family related to the 2-oxoglutarate/malate translocator from
RT spinach chloroplasts.";
RL J. Bacteriol. 180:4160-4165(1998).
CC -!- FUNCTION: Responsible for the uptake of citrate in exchange with the
CC efflux of succinate, fumarate or tartrate. Has a relatively broad
CC specificity for C(4)-dicarboxylates and tricarboxylates
CC (PubMed:9696764). {ECO:0000269|PubMed:9696764}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC DIT1 subfamily. {ECO:0000305}.
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DR EMBL; U82598; AAB40812.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73713.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35241.1; -; Genomic_DNA.
DR PIR; B64795; B64795.
DR RefSeq; NP_415145.1; NC_000913.3.
DR RefSeq; WP_000050323.1; NZ_SSZK01000032.1.
DR AlphaFoldDB; P0AE74; -.
DR SMR; P0AE74; -.
DR BioGRID; 4259902; 6.
DR STRING; 511145.b0612; -.
DR TCDB; 2.A.47.3.2; the divalent anion:na(+) symporter (dass) family.
DR PaxDb; P0AE74; -.
DR PRIDE; P0AE74; -.
DR EnsemblBacteria; AAC73713; AAC73713; b0612.
DR EnsemblBacteria; BAA35241; BAA35241; BAA35241.
DR GeneID; 66671113; -.
DR GeneID; 949070; -.
DR KEGG; ecj:JW0604; -.
DR KEGG; eco:b0612; -.
DR PATRIC; fig|1411691.4.peg.1656; -.
DR EchoBASE; EB3308; -.
DR eggNOG; COG0471; Bacteria.
DR HOGENOM; CLU_005170_7_0_6; -.
DR InParanoid; P0AE74; -.
DR OMA; GLNPNAW; -.
DR PhylomeDB; P0AE74; -.
DR BioCyc; EcoCyc:B0612-MON; -.
DR BioCyc; MetaCyc:B0612-MON; -.
DR PRO; PR:P0AE74; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR InterPro; IPR030676; CitT-rel.
DR InterPro; IPR001898; SLC13A/DASS.
DR PANTHER; PTHR42826; PTHR42826; 1.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR PIRSF; PIRSF002457; DASS; 1.
DR TIGRFAMs; TIGR00785; dass; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..487
FT /note="Citrate/succinate antiporter"
FT /id="PRO_0000172521"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 487 AA; 53093 MW; 9AD55B9E7448F1DB CRC64;
MSLAKDNIWK LLAPLVVMGV MFLIPVPDGM PPQAWHYFAV FVAMIVGMIL EPIPATAISF
IAVTICVIGS NYLLFDAKEL ADPAFNAQKQ ALKWGLAGFS STTVWLVFGA FIFALGYEVS
GLGRRIALFL VKFMGKRTLT LGYAIVIIDI LLAPFTPSNT ARTGGTVFPV IKNLPPLFKS
FPNDPSARRI GGYLMWMMVI STSLSSSMFV TGAAPNVLGL EFVSKIAGIQ ISWLQWFLCF
LPVGVILLII APWLSYVLYK PEITHSEEVA TWAGDELKTM GALTRREWTL IGLVLLSLGL
WVFGSEVINA TAVGLLAVSL MLALHVVPWK DITRYNSAWN TLVNLATLVV MANGLTRSGF
IDWFAGTMST HLEGFSPNAT VIVLVLVFYF AHYLFASLSA HTATMLPVIL AVGKGIPGVP
MEQLCILLVL SIGIMGCLTP YATGPGVIIY GCGYVKSKDY WRLGAIFGVI YISMLLLVGW
PILAMWN
