ID   CJ1C1_EPIJA             Reviewed;          54 AA.
AC   P0DPE5;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2018, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Toxin AnmTx Cj 1c-1 {ECO:0000303|PubMed:29109403};
DE   Flags: Precursor; Fragment;
OS   Epiactis japonica (Sea anemone) (Cnidopus japonicus).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Epiactis.
OX   NCBI_TaxID=58804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOASSAY, AND TOXIC DOSE.
RX   PubMed=29109403; DOI=10.1038/s41598-017-14961-1;
RA   Babenko V.V., Mikov A.N., Manuvera V.A., Anikanov N.A., Kovalchuk S.I.,
RA   Andreev Y.A., Logashina Y.A., Kornilov D.A., Manolov A.I., Sanamyan N.P.,
RA   Sanamyan K.E., Kostryukova E.S., Kozlov S.A., Grishin E.V., Govorun V.M.,
RA   Lazarev V.N.;
RT   "Identification of unusual peptides with new Cys frameworks in the venom of
RT   the cold-water sea anemone Cnidopus japonicus.";
RL   Sci. Rep. 7:14534-14534(2017).
CC   -!- FUNCTION: In vivo, induces marked paralysis on shrimps (C.multidentata)
CC       at 10-20 seconds after injection and a weak toxicity when injected into
CC       insect larvae (M.domestica). {ECO:0000269|PubMed:29109403}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:29109403}.
CC       Nematocyst {ECO:0000305}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(100) is 30 ug/g on the shrimp C.multidentata.
CC       {ECO:0000269|PubMed:29109403}.
CC   -!- TOXIC DOSE: LD(50) is 30 ug/g when injected into fly larvae
CC       (M.domestica). {ECO:0000269|PubMed:29109403}.
CC   -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC       family. Type I subfamily. {ECO:0000305}.
CC   -!- CAUTION: Toxicity tests have been done on recombinant non-amidated
CC       toxin. {ECO:0000305|PubMed:29109403}.
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DR   AlphaFoldDB; P0DPE5; -.
DR   SMR; P0DPE5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
PE   2: Evidence at transcript level;
KW   Amidation; Disulfide bond; Nematocyst; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          <1..7
FT                   /evidence="ECO:0000305"
FT   CHAIN           8..53
FT                   /note="Toxin AnmTx Cj 1c-1"
FT                   /evidence="ECO:0000305|PubMed:29109403"
FT                   /id="PRO_0000443394"
FT   MOD_RES         53
FT                   /note="Glutamic acid 1-amide"
FT                   /evidence="ECO:0000305"
FT   DISULFID        9..50
FT                   /evidence="ECO:0000250|UniProtKB:P0C280"
FT   DISULFID        11..41
FT                   /evidence="ECO:0000250|UniProtKB:P0C280"
FT   DISULFID        33..51
FT                   /evidence="ECO:0000250|UniProtKB:P0C280"
FT   NON_TER         1
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   54 AA;  5913 MW;  68B98B80F3868737 CRC64;
     MLNKRGVPCR CESDGPPRQN NALSGTTFYV VGCNKAGWNK CRYINAISTC CKEG