ID   CJTL8_EPIJA             Reviewed;          83 AA.
AC   P0DPE6;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2018, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Toxin CjTL8 {ECO:0000303|PubMed:29109403};
DE   Flags: Precursor;
OS   Epiactis japonica (Sea anemone) (Cnidopus japonicus).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Epiactis.
OX   NCBI_TaxID=58804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOASSAY, AND TOXIC DOSE.
RX   PubMed=29109403; DOI=10.1038/s41598-017-14961-1;
RA   Babenko V.V., Mikov A.N., Manuvera V.A., Anikanov N.A., Kovalchuk S.I.,
RA   Andreev Y.A., Logashina Y.A., Kornilov D.A., Manolov A.I., Sanamyan N.P.,
RA   Sanamyan K.E., Kostryukova E.S., Kozlov S.A., Grishin E.V., Govorun V.M.,
RA   Lazarev V.N.;
RT   "Identification of unusual peptides with new Cys frameworks in the venom of
RT   the cold-water sea anemone Cnidopus japonicus.";
RL   Sci. Rep. 7:14534-14534(2017).
CC   -!- FUNCTION: In vivo, induces immediate paralysis on shrimps
CC       (C.multidentata), followed by death when high doses are injected. No
CC       activity is observed when injected into fly larvae (M. domestica).
CC       {ECO:0000269|PubMed:29109403}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:29109403}.
CC       Nematocyst {ECO:0000305}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(100) is 10 ug/g on the shrimp C.multidentata.
CC       {ECO:0000269|PubMed:29109403}.
CC   -!- CAUTION: Toxicity tests have been done on recombinant non-amidated
CC       toxin. {ECO:0000305|PubMed:29109403}.
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DR   AlphaFoldDB; P0DPE6; -.
DR   SMR; P0DPE6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Amidation; Cleavage on pair of basic residues; Disulfide bond; Nematocyst;
KW   Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..44
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000443395"
FT   CHAIN           45..81
FT                   /note="Toxin CjTL8"
FT                   /evidence="ECO:0000305|PubMed:29109403"
FT                   /id="PRO_0000443396"
FT   MOD_RES         81
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   83 AA;  9199 MW;  1335AF9EC89150C9 CRC64;
     MSSAIKILAL LMVLVALAQA KPRKDYRAYP DFDDKSVILE DDKRCDPDKR DSVCKDVCGM
     LDIGTENGEC PGKEVCCVDL FGR