CK068_HUMAN
ID CK068_HUMAN Reviewed; 292 AA.
AC Q9H3H3; J3KQG9; Q9BT13;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-DEC-2018, sequence version 3.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=UPF0696 protein C11orf68;
DE AltName: Full=Basophilic leukemia-expressed protein Bles03;
DE AltName: Full=Protein p5326;
GN Name=C11orf68; Synonyms=BLES03;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 11-245 (ISOFORM 3), AND VARIANT ARG-195.
RC TISSUE=Adrenal cortex, Brain, Embryonic stem cell, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 22-292 (ISOFORM 2), AND VARIANT ARG-195.
RC TISSUE=Mesenchymal cell;
RA Zhang J.S.S., Reddel R.R.;
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-29 (ISOFORMS 2 AND 3), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Colon carcinoma;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 42-292.
RX PubMed=16511166; DOI=10.1107/s1744309105023845;
RA Bitto E., Bingman C.A., Robinson H., Allard S.T.M., Wesenberg G.E.,
RA Phillips G.N. Jr.;
RT "The structure at 2.5 A resolution of human basophilic leukemia-expressed
RT protein BLES03.";
RL Acta Crystallogr. F 61:812-817(2005).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 42-292.
RX PubMed=17850744; DOI=10.1016/j.str.2007.06.019;
RA Levin E.J., Kondrashov D.A., Wesenberg G.E., Phillips G.N. Jr.;
RT "Ensemble refinement of protein crystal structures: validation and
RT application.";
RL Structure 15:1040-1052(2007).
CC -!- INTERACTION:
CC Q9H3H3; Q15056: EIF4H; NbExp=5; IntAct=EBI-721765, EBI-748492;
CC Q9H3H3; Q15233: NONO; NbExp=3; IntAct=EBI-721765, EBI-350527;
CC Q9H3H3; Q04864: REL; NbExp=3; IntAct=EBI-721765, EBI-307352;
CC Q9H3H3; O00560: SDCBP; NbExp=3; IntAct=EBI-721765, EBI-727004;
CC Q9H3H3-3; Q15056-2: EIF4H; NbExp=8; IntAct=EBI-12002214, EBI-12222405;
CC Q9H3H3-3; Q9UIH9: KLF15; NbExp=3; IntAct=EBI-12002214, EBI-2796400;
CC Q9H3H3-3; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-12002214, EBI-16439278;
CC Q9H3H3-3; Q15233: NONO; NbExp=3; IntAct=EBI-12002214, EBI-350527;
CC Q9H3H3-3; Q8N4B1-4: PHETA1; NbExp=3; IntAct=EBI-12002214, EBI-14131832;
CC Q9H3H3-3; Q04864-2: REL; NbExp=3; IntAct=EBI-12002214, EBI-10829018;
CC Q9H3H3-3; O00560: SDCBP; NbExp=3; IntAct=EBI-12002214, EBI-727004;
CC Q9H3H3-3; Q9UL33-2: TRAPPC2L; NbExp=8; IntAct=EBI-12002214, EBI-11119202;
CC Q9H3H3-3; Q9BYN7: ZNF341; NbExp=3; IntAct=EBI-12002214, EBI-9089622;
CC Q9H3H3-3; Q96KM6: ZNF512B; NbExp=3; IntAct=EBI-12002214, EBI-1049952;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=2;
CC IsoId=Q9H3H3-2; Sequence=Displayed;
CC Name=1;
CC IsoId=Q9H3H3-1; Sequence=VSP_059970;
CC Name=3;
CC IsoId=Q9H3H3-3; Sequence=VSP_059971;
CC -!- MISCELLANEOUS: [Isoform 1]: Dubious isoform based on intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the UPF0696 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG43171.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH13185.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AP006287; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC004414; AAH04414.1; -; mRNA.
DR EMBL; BC010512; AAH10512.1; -; mRNA.
DR EMBL; BC013185; AAH13185.1; ALT_INIT; mRNA.
DR EMBL; CX787057; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AF073483; AAG43171.1; ALT_INIT; mRNA.
DR CCDS; CCDS44652.1; -. [Q9H3H3-3]
DR CCDS; CCDS8122.2; -. [Q9H3H3-2]
DR RefSeq; NP_001129107.1; NM_001135635.1. [Q9H3H3-3]
DR RefSeq; NP_113638.2; NM_031450.3. [Q9H3H3-2]
DR PDB; 1ZTP; X-ray; 2.50 A; A/B/C=42-292.
DR PDB; 2Q4K; X-ray; 2.50 A; A/B/C=42-292.
DR PDBsum; 1ZTP; -.
DR PDBsum; 2Q4K; -.
DR AlphaFoldDB; Q9H3H3; -.
DR SMR; Q9H3H3; -.
DR BioGRID; 123702; 19.
DR IntAct; Q9H3H3; 16.
DR MINT; Q9H3H3; -.
DR STRING; 9606.ENSP00000398350; -.
DR iPTMnet; Q9H3H3; -.
DR PhosphoSitePlus; Q9H3H3; -.
DR BioMuta; C11orf68; -.
DR DMDM; 325511329; -.
DR EPD; Q9H3H3; -.
DR jPOST; Q9H3H3; -.
DR MassIVE; Q9H3H3; -.
DR MaxQB; Q9H3H3; -.
DR PaxDb; Q9H3H3; -.
DR PeptideAtlas; Q9H3H3; -.
DR PRIDE; Q9H3H3; -.
DR ProteomicsDB; 80714; -. [Q9H3H3-1]
DR ProteomicsDB; 80715; -. [Q9H3H3-2]
DR ProteomicsDB; 80716; -. [Q9H3H3-3]
DR Antibodypedia; 51800; 44 antibodies from 11 providers.
DR DNASU; 83638; -.
DR Ensembl; ENST00000438576.3; ENSP00000398350.2; ENSG00000175573.7. [Q9H3H3-3]
DR Ensembl; ENST00000449692.3; ENSP00000409681.3; ENSG00000175573.7. [Q9H3H3-2]
DR Ensembl; ENST00000530188.1; ENSP00000433914.1; ENSG00000175573.7. [Q9H3H3-1]
DR GeneID; 83638; -.
DR KEGG; hsa:83638; -.
DR MANE-Select; ENST00000438576.3; ENSP00000398350.2; NM_001135635.2; NP_001129107.1. [Q9H3H3-3]
DR UCSC; uc001ogi.4; human. [Q9H3H3-2]
DR CTD; 83638; -.
DR DisGeNET; 83638; -.
DR GeneCards; C11orf68; -.
DR HGNC; HGNC:28801; C11orf68.
DR HPA; ENSG00000175573; Low tissue specificity.
DR neXtProt; NX_Q9H3H3; -.
DR OpenTargets; ENSG00000175573; -.
DR PharmGKB; PA144596487; -.
DR VEuPathDB; HostDB:ENSG00000175573; -.
DR eggNOG; ENOG502QRQJ; Eukaryota.
DR GeneTree; ENSGT00390000011640; -.
DR HOGENOM; CLU_051869_1_0_1; -.
DR InParanoid; Q9H3H3; -.
DR OMA; TGKWLMH; -.
DR OrthoDB; 1023014at2759; -.
DR PhylomeDB; Q9H3H3; -.
DR TreeFam; TF331797; -.
DR PathwayCommons; Q9H3H3; -.
DR SignaLink; Q9H3H3; -.
DR BioGRID-ORCS; 83638; 20 hits in 1065 CRISPR screens.
DR ChiTaRS; C11orf68; human.
DR EvolutionaryTrace; Q9H3H3; -.
DR GenomeRNAi; 83638; -.
DR Pharos; Q9H3H3; Tdark.
DR PRO; PR:Q9H3H3; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9H3H3; protein.
DR Bgee; ENSG00000175573; Expressed in right hemisphere of cerebellum and 197 other tissues.
DR Genevisible; Q9H3H3; HS.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR Gene3D; 3.30.760.10; -; 1.
DR InterPro; IPR015034; Bles03.
DR InterPro; IPR023398; TIF_eIF4e-like.
DR PANTHER; PTHR31977; PTHR31977; 1.
DR Pfam; PF08939; DUF1917; 1.
DR SUPFAM; SSF55418; SSF55418; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Methylation; Reference proteome.
FT CHAIN 1..292
FT /note="UPF0696 protein C11orf68"
FT /id="PRO_0000228117"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 29
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 1..41
FT /note="Missing (in isoform 1)"
FT /id="VSP_059970"
FT VAR_SEQ 40
FT /note="R -> RS (in isoform 3)"
FT /id="VSP_059971"
FT VARIANT 195
FT /note="Q -> R (in dbSNP:rs7947504)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3"
FT /id="VAR_060319"
FT HELIX 59..61
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 63..69
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:1ZTP"
FT TURN 83..85
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 88..90
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 91..97
FT /evidence="ECO:0007829|PDB:1ZTP"
FT TURN 100..102
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 120..123
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 134..147
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 153..163
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 168..174
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 178..192
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 197..201
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 206..208
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 211..218
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 223..235
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 242..246
FT /evidence="ECO:0007829|PDB:1ZTP"
FT HELIX 247..251
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 266..271
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 274..277
FT /evidence="ECO:0007829|PDB:1ZTP"
FT STRAND 280..284
FT /evidence="ECO:0007829|PDB:1ZTP"
FT TURN 285..288
FT /evidence="ECO:0007829|PDB:1ZTP"
SQ SEQUENCE 292 AA; 31430 MW; 4FE98CC31A406CDA CRC64;
MAAAAAAAVA GVGRGGGGAE PRQERSRARG WAGVERSEGR RMEPGEELEE EGSPGGREDG
FTAEHLAAEA MAADMDPWLV FDARTTPATE LDAWLAKYPP SQVTRYGDPG SPNSEPVGWI
AVYGQGYSPN SGDVQGLQAA WEALQTSGRP ITPGTLRQLA ITHHVLSGKW LMHLAPGFKL
DHAWAGIARA VVEGQLQVAK VSPRAKEGGR QVICVYTDDF TDRLGVLEAD SAIRAAGIKC
LLTYKPDVYT YLGIYRANRW HLCPTLYESR FQLGGSARGS RVLDRANNVE LT
