ACHA_DANRE
ID ACHA_DANRE Reviewed; 456 AA.
AC Q98880;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Acetylcholine receptor subunit alpha;
DE Flags: Precursor;
GN Name=chrna1; Synonyms=nic1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9475746; DOI=10.1093/genetics/148.1.361;
RA Sepich D.S., Wegner J., O'Shea S., Westerfield M.;
RT "An altered intron inhibits synthesis of the acetylcholine receptor alpha-
RT subunit in the paralyzed zebrafish mutant nic1.";
RL Genetics 148:361-372(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Upon acetylcholine binding, the AChR responds by an extensive
CC change in conformation that affects all subunits and leads to opening
CC of an ion-conducting channel across the plasma membrane.
CC {ECO:0000250|UniProtKB:P02708}.
CC -!- SUBUNIT: One of the alpha chains that assemble within the acetylcholine
CC receptor, a pentamer of two alpha chains, a beta, a delta, and a gamma
CC or epsilon chains. {ECO:0000250|UniProtKB:P02708}.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane
CC {ECO:0000250|UniProtKB:P02708}; Multi-pass membrane protein
CC {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:P02708}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; AH003668; AAB09701.1; -; Genomic_DNA.
DR EMBL; U70437; AAB09770.1; -; Genomic_DNA.
DR EMBL; U70438; AAB16917.1; -; mRNA.
DR EMBL; BC081554; AAH81554.1; -; mRNA.
DR RefSeq; NP_571520.1; NM_131445.1.
DR AlphaFoldDB; Q98880; -.
DR SMR; Q98880; -.
DR STRING; 7955.ENSDARP00000021860; -.
DR PaxDb; Q98880; -.
DR Ensembl; ENSDART00000020261; ENSDARP00000021860; ENSDARG00000009021.
DR GeneID; 30725; -.
DR KEGG; dre:30725; -.
DR CTD; 1134; -.
DR ZFIN; ZDB-GENE-980526-137; chrna1.
DR eggNOG; KOG3645; Eukaryota.
DR GeneTree; ENSGT00940000156851; -.
DR HOGENOM; CLU_018074_1_0_1; -.
DR InParanoid; Q98880; -.
DR OMA; STHIMPA; -.
DR OrthoDB; 381858at2759; -.
DR PhylomeDB; Q98880; -.
DR TreeFam; TF315605; -.
DR Reactome; R-DRE-629594; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
DR Reactome; R-DRE-629597; Highly calcium permeable nicotinic acetylcholine receptors.
DR PRO; PR:Q98880; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000009021; Expressed in muscle tissue and 23 other tissues.
DR ExpressionAtlas; Q98880; baseline.
DR GO; GO:0005892; C:acetylcholine-gated channel complex; IDA:ZFIN.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0022848; F:acetylcholine-gated cation-selective channel activity; IBA:GO_Central.
DR GO; GO:0005261; F:cation channel activity; IDA:ZFIN.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0003428; P:chondrocyte intercalation involved in growth plate cartilage morphogenesis; IMP:ZFIN.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0086009; P:membrane repolarization; IMP:ZFIN.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:ZFIN.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0007274; P:neuromuscular synaptic transmission; IDA:ZFIN.
DR GO; GO:0043113; P:receptor clustering; IMP:ZFIN.
DR GO; GO:0030516; P:regulation of axon extension; IMP:ZFIN.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0048741; P:skeletal muscle fiber development; IMP:ZFIN.
DR GO; GO:0048705; P:skeletal system morphogenesis; IMP:ZFIN.
DR GO; GO:0007271; P:synaptic transmission, cholinergic; IDA:ZFIN.
DR Gene3D; 1.20.58.390; -; 2.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 2.
DR PRINTS; PR00254; NICOTINICR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000250"
FT CHAIN 21..456
FT /note="Acetylcholine receptor subunit alpha"
FT /id="PRO_0000000309"
FT TOPO_DOM 21..230
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..428
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 148..162
FT /evidence="ECO:0000250"
FT DISULFID 212..213
FT /note="Associated with receptor activation"
FT /evidence="ECO:0000250"
SQ SEQUENCE 456 AA; 52100 MW; 336825C5E358C6F3 CRC64;
MNYFILILPI LPYLYGPAVC SEDETRLVKT LFTGYNKVVR PVSHFKDPVV VTVGLQLIQL
ISVDEVNQIV TSNVRLKQQW KDVHLQWNPD DYGGIRKIRI PSTDLWKPDL VLYNNADGDF
AIVHETKVLL EHTGMITWTP PAIFKSYCEI VVLHFPFDLQ NCSMKLGTWT YDGNLVIINP
DSDRPDLSNF MESGEWVMKD YRSWKHWVYY ACCPDTPYLD ITYHFLLLRL PLYFIVNVII
PCMLFSFLTG LVFYLPTDSG EKMTLSISVL LSLTVFLLVI VELIPSTSSA VPLIGKYMLF
TMIFVIASII ITVIVINTHH RSPSTHIMPA WVRKIFIDTI PNMMFFSTMK RPSQERQEKR
LFPADFDISD ISGKPMPASV TYHSPITKNP DVRSAIEGVK YIADTMKSDE ESNNAAEEWK
FVAMVLDHIL LCVFMAVCII GTLGVFAGRL IELSML
