ID   CK2N2_RAT               Reviewed;          79 AA.
AC   Q9Z2N6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Calcium/calmodulin-dependent protein kinase II inhibitor 2;
DE   AltName: Full=CaM-KII inhibitory protein;
DE            Short=CaM-KIIN;
DE   AltName: Full=CaM-KIINbeta;
GN   Name=Camk2n2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CAMK2A AND CAMK2B,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Brain;
RX   PubMed=9724800; DOI=10.1073/pnas.95.18.10890;
RA   Chang B.H., Mukherji S., Soderling T.R.;
RT   "Characterization of a calmodulin kinase II inhibitor protein in brain.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:10890-10895(1998).
RN   [2]
RP   INTERACTION WITH CAMK2B, AND TISSUE SPECIFICITY.
RX   PubMed=11182241; DOI=10.1016/s0306-4522(00)00520-0;
RA   Chang B.H., Mukherji S., Soderling T.R.;
RT   "Calcium/calmodulin-dependent protein kinase II inhibitor protein:
RT   localization of isoforms in rat brain.";
RL   Neuroscience 102:767-777(2001).
RN   [3]
RP   FUNCTION.
RX   PubMed=17942605; DOI=10.1091/mbc.e07-02-0185;
RA   Vest R.S., Davies K.D., O'Leary H., Port J.D., Bayer K.U.;
RT   "Dual mechanism of a natural CaMKII inhibitor.";
RL   Mol. Biol. Cell 18:5024-5033(2007).
CC   -!- FUNCTION: Potent and specific cellular inhibitor of CaM-kinase II
CC       (CAMK2) (PubMed:9724800, PubMed:17942605). Traps Ca(2+)/calmodulin on
CC       CAMK2 (PubMed:17942605). {ECO:0000269|PubMed:17942605,
CC       ECO:0000269|PubMed:9724800}.
CC   -!- SUBUNIT: Interacts with CAMK2A and CAMK2B in the presence of
CC       Ca(2+)/calmodulin or after autophosphorylation.
CC       {ECO:0000269|PubMed:11182241, ECO:0000269|PubMed:9724800}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9724800}. Cytoplasm,
CC       cytosol {ECO:0000269|PubMed:9724800}. Synapse
CC       {ECO:0000250|UniProtKB:Q78WH7}. Note=Excluded from nucleus when
CC       coexpressed with activated CAMK2. {ECO:0000269|PubMed:9724800}.
CC   -!- TISSUE SPECIFICITY: Expressed in forebrain, hippocampus, midbrain,
CC       cerebellum, and testis. Expressed in forebrain, hippocampus, midbrain,
CC       and cerebellum (at protein level). In the cortex expressed more in
CC       laminae II and III than in laminae IV-VI. Expressed within the
CC       pyramidal layer of the hippocampus and granular layer of the dentate
CC       gyrus as well as in the olfactory bulb. Diffusely expressed within the
CC       caudate-putamen and globus pallidus. Diffusely expressed throughout
CC       regions of the midbrain. In cerebellum, expressed within the Purkinje
CC       and granular layer. Stronger expressed in the cerebellum. Highly
CC       expressed in frontal cortex, hippocampus, olfactory bulb, caudate-
CC       putamen and cerebellum exhibit (at protein level).
CC       {ECO:0000269|PubMed:11182241, ECO:0000269|PubMed:9724800}.
CC   -!- SIMILARITY: Belongs to the CAMK2N family. {ECO:0000305}.
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DR   EMBL; AF041854; AAD02202.1; -; mRNA.
DR   RefSeq; NP_067710.1; NM_021678.1.
DR   AlphaFoldDB; Q9Z2N6; -.
DR   STRING; 10116.ENSRNOP00000054937; -.
DR   PaxDb; Q9Z2N6; -.
DR   GeneID; 59314; -.
DR   KEGG; rno:59314; -.
DR   CTD; 94032; -.
DR   RGD; 708411; Camk2n2.
DR   eggNOG; ENOG502S4FG; Eukaryota.
DR   HOGENOM; CLU_197183_0_0_1; -.
DR   InParanoid; Q9Z2N6; -.
DR   OMA; MSEIMPY; -.
DR   OrthoDB; 1637105at2759; -.
DR   PhylomeDB; Q9Z2N6; -.
DR   PRO; PR:Q9Z2N6; -.
DR   Proteomes; UP000002494; Chromosome 11.
DR   Bgee; ENSRNOG00000038184; Expressed in cerebellum and 15 other tissues.
DR   Genevisible; Q9Z2N6; RN.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR   GO; GO:0008427; F:calcium-dependent protein kinase inhibitor activity; IMP:RGD.
DR   GO; GO:0019901; F:protein kinase binding; IMP:RGD.
DR   GO; GO:0006469; P:negative regulation of protein kinase activity; IBA:GO_Central.
DR   GO; GO:0048167; P:regulation of synaptic plasticity; TAS:RGD.
DR   InterPro; IPR026779; Camk2n.
DR   PANTHER; PTHR31007; PTHR31007; 1.
DR   Pfam; PF15170; CaM-KIIN; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Protein kinase inhibitor; Reference proteome; Synapse.
FT   CHAIN           1..79
FT                   /note="Calcium/calmodulin-dependent protein kinase II
FT                   inhibitor 2"
FT                   /id="PRO_0000327268"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          43..69
FT                   /note="Inhibitory domain"
SQ   SEQUENCE   79 AA;  8628 MW;  B34820F5D6417CF3 CRC64;
     MSEILPYGED KMGRFGADPE GSDLSFSCRL QDTNSFFAGN QAKRPPKLGQ IGRAKRVVIE
     DDRIDDVLKG MGEKPPSGV