CKI1_CAEEL
ID CKI1_CAEEL Reviewed; 184 AA.
AC Q22197;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Cyclin-dependent kinase inhibitor 1;
GN Name=cki-1 {ECO:0000312|WormBase:T05A6.1};
GN ORFNames=T05A6.1 {ECO:0000312|WormBase:T05A6.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Bristol N2 {ECO:0000269|PubMed:10587644};
RX PubMed=10587644; DOI=10.1038/70272;
RA Feng H., Zhong W., Punkosdy G., Gu S., Zhou L., Seabolt E.K., Kipreos E.T.;
RT "CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome
RT condensation in Caenorhabditis elegans.";
RL Nat. Cell Biol. 1:486-492(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=9716524; DOI=10.1242/dev.125.18.3585;
RA Hong Y., Roy R., Ambros V.;
RT "Developmental regulation of a cyclin-dependent kinase inhibitor controls
RT postembryonic cell cycle progression in Caenorhabditis elegans.";
RL Development 125:3585-3597(1998).
RN [4]
RP INTERACTION WITH CYD-1, AND DISRUPTION PHENOTYPE.
RX PubMed=11684669; DOI=10.1242/dev.128.21.4349;
RA Boxem M., van den Heuvel S.;
RT "lin-35 Rb and cki-1 Cip/Kip cooperate in developmental regulation of G1
RT progression in C. elegans.";
RL Development 128:4349-4359(2001).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=15247923; DOI=10.1038/ncb1154;
RA Saito R.M., Perreault A., Peach B., Satterlee J.S., van den Heuvel S.;
RT "The CDC-14 phosphatase controls developmental cell-cycle arrest in C.
RT elegans.";
RL Nat. Cell Biol. 6:777-783(2004).
RN [6]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=17476329; DOI=10.1371/journal.pone.0000407;
RA Fujita M., Takeshita H., Sawa H.;
RT "Cyclin E and CDK2 repress the terminal differentiation of quiescent cells
RT after asymmetric division in C. elegans.";
RL PLoS ONE 2:E407-E407(2007).
CC -!- FUNCTION: Negative cell-cycle regulator that functions at the G1-to-S-
CC phase transition (PubMed:10587644, PubMed:9716524). Required for
CC suspension of the cell cycle in dauer larvae and starved L1 larvae
CC (PubMed:10587644, PubMed:9716524). In vulval precursor cells (VPCs), a
CC pathway of heterochronic genes acts via cki-1 to maintain VPCs in G1
CC during the L2 larval stage (PubMed:10587644, PubMed:9716524,
CC PubMed:15247923). Cul-2 may function in ubiquitin-mediated degradation
CC by targeting cki-1 for degradation (PubMed:10587644). Involved in
CC distal tip cell development by repressing and modulating cye-1/cdk-2
CC activity levels in Z1.aa/Z4.pp and in Z1.ap/Z4.pa (PubMed:17476329).
CC {ECO:0000269|PubMed:10587644, ECO:0000269|PubMed:15247923,
CC ECO:0000269|PubMed:17476329, ECO:0000269|PubMed:9716524}.
CC -!- SUBUNIT: Interacts with cyd-1 (Probable); the interaction is direct.
CC {ECO:0000269|PubMed:11684669}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10587644,
CC ECO:0000269|PubMed:15247923}.
CC -!- TISSUE SPECIFICITY: In embryos, expression is first seen in pharyngeal
CC primordium and later in all differentiating cells. Post embryonic
CC expression corresponds to developmental patterns of cell cycle
CC progression in many tissues including sex myoblasts, distal tip cells,
CC vulval cells, seam cells, neurons, intestine cells and hypodermal
CC cells. {ECO:0000269|PubMed:10587644, ECO:0000269|PubMed:9716524}.
CC -!- DEVELOPMENTAL STAGE: In embryos, expression is first seen in pharyngeal
CC primordium and later in all differentiating cells. Post embryonic
CC expression corresponds to developmental patterns of cell cycle
CC progression in many tissues including sex myoblasts, distal tip cells,
CC vulval cells, seam cells, neurons, intestine cells and hypodermal cells
CC (PubMed:10587644, PubMed:9716524). During the development of distal tip
CC cells (DTC), expressed asymmetrically between the daughters of the Z1.a
CC and Z4.p cells; asymmetric expression is regulated by wrm-1, a
CC component of the Wnt/MAPK pathway (PubMed:17476329).
CC {ECO:0000269|PubMed:10587644, ECO:0000269|PubMed:17476329,
CC ECO:0000269|PubMed:9716524}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in additional
CC cell divisions in postembryonic intestinal cells characterized by a
CC premature entry into S phase. {ECO:0000269|PubMed:11684669,
CC ECO:0000269|PubMed:15247923}.
CC -!- SIMILARITY: Belongs to the CDI family. {ECO:0000305}.
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DR EMBL; AF179358; AAF13868.1; -; mRNA.
DR EMBL; BX284602; CAA90667.1; -; Genomic_DNA.
DR PIR; T24496; T24496.
DR RefSeq; NP_495641.1; NM_063240.4.
DR AlphaFoldDB; Q22197; -.
DR SMR; Q22197; -.
DR BioGRID; 39593; 7.
DR STRING; 6239.T05A6.1; -.
DR PaxDb; Q22197; -.
DR EnsemblMetazoa; T05A6.1.1; T05A6.1.1; WBGene00000516.
DR GeneID; 174260; -.
DR KEGG; cel:CELE_T05A6.1; -.
DR UCSC; T05A6.1; c. elegans.
DR CTD; 174260; -.
DR WormBase; T05A6.1; CE03628; WBGene00000516; cki-1.
DR eggNOG; KOG4743; Eukaryota.
DR HOGENOM; CLU_1483285_0_0_1; -.
DR InParanoid; Q22197; -.
DR OMA; AKREHAT; -.
DR OrthoDB; 1576305at2759; -.
DR PRO; PR:Q22197; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00000516; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0008406; P:gonad development; IGI:UniProtKB.
DR GO; GO:0045596; P:negative regulation of cell differentiation; IMP:UniProtKB.
DR GO; GO:0051782; P:negative regulation of cell division; IMP:UniProtKB.
DR GO; GO:0045605; P:negative regulation of epidermal cell differentiation; IMP:UniProtKB.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IMP:UniProtKB.
DR GO; GO:1903452; P:positive regulation of G1 to G0 transition; IMP:WormBase.
DR GO; GO:1902806; P:regulation of cell cycle G1/S phase transition; IGI:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IMP:WormBase.
DR GO; GO:0051302; P:regulation of cell division; IGI:UniProtKB.
DR GO; GO:0032875; P:regulation of DNA endoreduplication; IGI:UniProtKB.
DR Gene3D; 4.10.365.10; -; 1.
DR InterPro; IPR003175; CDI_dom.
DR InterPro; IPR044898; CDI_dom_sf.
DR Pfam; PF02234; CDI; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Nucleus; Protein kinase inhibitor; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..184
FT /note="Cyclin-dependent kinase inhibitor 1"
FT /id="PRO_0000190089"
FT REGION 85..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 85..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..184
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 184 AA; 21245 MW; 184D794528E2AAE2 CRC64;
MSSARRCLFG RPTPEQRSRT RIWLEDAVKR MRQEESQKWG FDFELETPLP SSAGFVYEVI
PENCVPEFYR TKVLTVRTTC SSLDISSTTL TPLSSPSTSD KEEPSLMDPN SSFEDEEEPK
KWQFREPPTP RKTPTKRQQK MTDFMAVSRK KNSLSPNKLS PVNVIFTPKS RRPTIRTRSS
CSPY
