CKLF_HUMAN
ID CKLF_HUMAN Reviewed; 152 AA.
AC Q9UBR5; C9JE38; Q9UHM7; Q9UHN8; Q9UI41;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Chemokine-like factor;
DE AltName: Full=C32;
GN Name=CKLF; Synonyms=CKLF1; ORFNames=HSPC224, UNQ410/PRO772;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606 {ECO:0000312|EMBL:AAF36144.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND ACTIVITY REGULATION.
RX PubMed=11415443; DOI=10.1042/0264-6021:3570127;
RA Han W., Lou Y., Tang J., Zhang Y., Chen Y., Li Y., Gu W., Huang J., Gui L.,
RA Tang Y., Li F., Song Q., Di C., Wang L., Shi Q., Sun R., Xia D., Rui M.,
RA Tang J., Ma D.;
RT "Molecular cloning and characterization of chemokine-like factor 1 (CKLF1),
RT a novel human cytokine with unique structure and potential chemotactic
RT activity.";
RL Biochem. J. 357:127-135(2001).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Pancreas {ECO:0000305};
RA Zhang J.S., Nelson M., Wang L., Smith D.I.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for 300
RT previously undefined genes expressed in CD34+ hematopoietic stem/progenitor
RT cells.";
RL Genome Res. 10:1546-1560(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RG SeattleSNPs variation discovery resource;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [7] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Pancreas {ECO:0000312|EMBL:AAH04380.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP SUBCELLULAR LOCATION (ISOFORM 1), AND FUNCTION (ISOFORM 1).
RX PubMed=16137713; DOI=10.1016/j.lfs.2005.05.070;
RA Wang Y., Zhang Y., Yang X., Han W., Liu Y., Xu Q., Zhao R., Di C., Song Q.,
RA Ma D.;
RT "Chemokine-like factor 1 is a functional ligand for CC chemokine receptor 4
RT (CCR4).";
RL Life Sci. 78:614-621(2006).
CC -!- FUNCTION: May play an important role in inflammation and regeneration
CC of skeletal muscle (PubMed:11415443). Essential for embryonic
CC development (By similarity). {ECO:0000250|UniProtKB:Q9DAS1,
CC ECO:0000269|PubMed:11415443}.
CC -!- FUNCTION: [Isoform 1]: Has chemotactic response in monocytes,
CC neutrophils and lymphocytes (PubMed:11415443). Binds CCR4
CC (PubMed:16137713). {ECO:0000269|PubMed:11415443,
CC ECO:0000269|PubMed:16137713}.
CC -!- ACTIVITY REGULATION: Partly inhibited by interleukin 10.
CC {ECO:0000269|PubMed:11415443}.
CC -!- INTERACTION:
CC Q9UBR5; O75581: LRP6; NbExp=3; IntAct=EBI-17572009, EBI-910915;
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Secreted
CC {ECO:0000269|PubMed:11415443, ECO:0000269|PubMed:16137713}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Isoform 4]: Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=2; Synonyms=CKLF2 {ECO:0000269|PubMed:11415443};
CC IsoId=Q9UBR5-1; Sequence=Displayed;
CC Name=1; Synonyms=CKLF1 {ECO:0000269|PubMed:11415443};
CC IsoId=Q9UBR5-2; Sequence=VSP_050605;
CC Name=3; Synonyms=CKLF3 {ECO:0000269|PubMed:11415443};
CC IsoId=Q9UBR5-3; Sequence=VSP_050605, VSP_050606;
CC Name=4; Synonyms=CKLF4 {ECO:0000269|PubMed:11415443};
CC IsoId=Q9UBR5-4; Sequence=VSP_050606;
CC Name=5;
CC IsoId=Q9UBR5-5; Sequence=VSP_041445, VSP_041446;
CC -!- TISSUE SPECIFICITY: Isoform 1, isoform 2, isoform 3 and isoform 4 have
CC highest expression levels in adult spleen, lung, testis, ovary,
CC peripheral blood leukocyte, placenta, pancreas, and in fetal brain,
CC skeletal muscle, thymus and heart. Lower expression levels in adult
CC skeletal muscle, liver, thymus colon, prostate and fetal spleen and
CC liver. {ECO:0000269|PubMed:11415443}.
CC -!- SIMILARITY: Belongs to the chemokine-like factor family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=SeattleSNPs;
CC URL="http://pga.gs.washington.edu/data/cklf/";
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DR EMBL; AF135380; AAF19599.1; -; mRNA.
DR EMBL; AF135381; AAF19600.1; -; mRNA.
DR EMBL; AF145216; AAF19350.1; -; mRNA.
DR EMBL; AF096895; AAF06722.1; -; mRNA.
DR EMBL; AF057306; AAF21255.1; -; mRNA.
DR EMBL; AF151058; AAF36144.1; -; mRNA.
DR EMBL; AY358999; AAQ89358.1; -; mRNA.
DR EMBL; AY665769; AAT70694.1; -; Genomic_DNA.
DR EMBL; AC010542; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC004380; AAH04380.1; -; mRNA.
DR CCDS; CCDS10806.1; -. [Q9UBR5-3]
DR CCDS; CCDS10807.1; -. [Q9UBR5-1]
DR CCDS; CCDS10808.1; -. [Q9UBR5-2]
DR CCDS; CCDS10809.1; -. [Q9UBR5-4]
DR CCDS; CCDS45502.1; -. [Q9UBR5-5]
DR RefSeq; NP_001035228.1; NM_001040138.2. [Q9UBR5-5]
DR RefSeq; NP_057410.1; NM_016326.3. [Q9UBR5-3]
DR RefSeq; NP_058647.1; NM_016951.3. [Q9UBR5-1]
DR RefSeq; NP_857591.1; NM_181640.2. [Q9UBR5-2]
DR RefSeq; NP_857592.1; NM_181641.2. [Q9UBR5-4]
DR AlphaFoldDB; Q9UBR5; -.
DR BioGRID; 119366; 5.
DR IntAct; Q9UBR5; 3.
DR STRING; 9606.ENSP00000264001; -.
DR iPTMnet; Q9UBR5; -.
DR PhosphoSitePlus; Q9UBR5; -.
DR SwissPalm; Q9UBR5; -.
DR BioMuta; CKLF; -.
DR DMDM; 34098753; -.
DR EPD; Q9UBR5; -.
DR jPOST; Q9UBR5; -.
DR MassIVE; Q9UBR5; -.
DR MaxQB; Q9UBR5; -.
DR PaxDb; Q9UBR5; -.
DR PeptideAtlas; Q9UBR5; -.
DR PRIDE; Q9UBR5; -.
DR ProteomicsDB; 84039; -. [Q9UBR5-1]
DR ProteomicsDB; 84040; -. [Q9UBR5-2]
DR ProteomicsDB; 84041; -. [Q9UBR5-3]
DR ProteomicsDB; 84042; -. [Q9UBR5-4]
DR Antibodypedia; 29261; 124 antibodies from 22 providers.
DR DNASU; 51192; -.
DR Ensembl; ENST00000264001.9; ENSP00000264001.5; ENSG00000217555.13. [Q9UBR5-1]
DR Ensembl; ENST00000345436.8; ENSP00000290771.4; ENSG00000217555.13. [Q9UBR5-4]
DR Ensembl; ENST00000351137.8; ENSP00000264003.4; ENSG00000217555.13. [Q9UBR5-2]
DR Ensembl; ENST00000362093.4; ENSP00000355417.4; ENSG00000217555.13. [Q9UBR5-3]
DR Ensembl; ENST00000417030.2; ENSP00000416678.2; ENSG00000217555.13. [Q9UBR5-5]
DR GeneID; 51192; -.
DR KEGG; hsa:51192; -.
DR MANE-Select; ENST00000264001.9; ENSP00000264001.5; NM_016951.4; NP_058647.1.
DR UCSC; uc002eow.4; human. [Q9UBR5-1]
DR CTD; 51192; -.
DR DisGeNET; 51192; -.
DR GeneCards; CKLF; -.
DR HGNC; HGNC:13253; CKLF.
DR HPA; ENSG00000217555; Low tissue specificity.
DR MIM; 616074; gene.
DR neXtProt; NX_Q9UBR5; -.
DR OpenTargets; ENSG00000217555; -.
DR PharmGKB; PA26531; -.
DR VEuPathDB; HostDB:ENSG00000217555; -.
DR eggNOG; KOG4788; Eukaryota.
DR GeneTree; ENSGT00940000162264; -.
DR HOGENOM; CLU_108546_3_0_1; -.
DR InParanoid; Q9UBR5; -.
DR OMA; CCSVKGH; -.
DR PhylomeDB; Q9UBR5; -.
DR TreeFam; TF317387; -.
DR PathwayCommons; Q9UBR5; -.
DR SignaLink; Q9UBR5; -.
DR BioGRID-ORCS; 51192; 11 hits in 1057 CRISPR screens.
DR ChiTaRS; CKLF; human.
DR GeneWiki; CKLF_(gene); -.
DR GenomeRNAi; 51192; -.
DR Pharos; Q9UBR5; Tbio.
DR PRO; PR:Q9UBR5; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9UBR5; protein.
DR Bgee; ENSG00000217555; Expressed in monocyte and 104 other tissues.
DR ExpressionAtlas; Q9UBR5; baseline and differential.
DR Genevisible; Q9UBR5; HS.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0008009; F:chemokine activity; IDA:UniProtKB.
DR GO; GO:0048247; P:lymphocyte chemotaxis; IDA:UniProtKB.
DR GO; GO:0048246; P:macrophage chemotaxis; IDA:UniProtKB.
DR GO; GO:0030593; P:neutrophil chemotaxis; IDA:UniProtKB.
DR GO; GO:0032940; P:secretion by cell; IDA:UniProtKB.
DR InterPro; IPR008253; Marvel.
DR PROSITE; PS51225; MARVEL; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chemotaxis; Cytokine; Membrane; Reference proteome;
KW Secreted; Transmembrane; Transmembrane helix.
FT CHAIN 1..152
FT /note="Chemokine-like factor"
FT /id="PRO_0000186094"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 13..133
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT VAR_SEQ 27..79
FT /note="Missing (in isoform 1 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:11415443"
FT /id="VSP_050605"
FT VAR_SEQ 80..111
FT /note="Missing (in isoform 4 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:11415443"
FT /id="VSP_050606"
FT VAR_SEQ 112..113
FT /note="VF -> LF (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_041445"
FT VAR_SEQ 114..152
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_041446"
SQ SEQUENCE 152 AA; 17170 MW; 6149D6D2B6AED9F3 CRC64;
MDNVQPKIKH RPFCFSVKGH VKMLRLALTV TSMTFFIIAQ APEPYIVITG FEVTVILFFI
LLYVLRLDRL MKWLFWPLLD IINSLVTTVF MLIVSVLALI PETTTLTVGG GVFALVTAVC
CLADGALIYR KLLFNPSGPY QKKPVHEKKE VL
