CKLF_MOUSE
ID CKLF_MOUSE Reviewed; 152 AA.
AC Q9DAS1; Q3T9V7; Q8BUC3; Q91ZA4; Q923S4; Q923S5;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Chemokine-like factor;
GN Name=Cklf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:BAB24133.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS CKLF2 AND CKLF3).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Corpora quadrigemina, Embryonic head, Liver, Spleen, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CKLF2; CKLF4 AND CKLF5).
RC STRAIN=BALB/cJ {ECO:0000305};
RA Rui M., Han W.L., Xia D.L., Liu Y., Zhang Y.M., Song Q.S., Di C.H.,
RA Ma D.L.;
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=34446558; DOI=10.1073/pnas.2106673118;
RA Miyata H., Oura S., Morohoshi A., Shimada K., Mashiko D., Oyama Y.,
RA Kaneda Y., Matsumura T., Abbasi F., Ikawa M.;
RT "SPATA33 localizes calcineurin to the mitochondria and regulates sperm
RT motility in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC -!- FUNCTION: May play an important role in inflammation and regeneration
CC of skeletal muscle (By similarity). Essential for embryonic development
CC (PubMed:34446558). {ECO:0000250|UniProtKB:Q9UBR5,
CC ECO:0000269|PubMed:34446558}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=CKLF2;
CC IsoId=Q9DAS1-1; Sequence=Displayed;
CC Name=CKLF3 {ECO:0000305};
CC IsoId=Q9DAS1-2; Sequence=VSP_050600, VSP_050602;
CC Name=CKLF4; Synonyms=mCKLF4;
CC IsoId=Q9DAS1-4; Sequence=VSP_050599;
CC Name=CKLF5;
CC IsoId=Q9DAS1-5; Sequence=VSP_050603;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:34446558}.
CC -!- DISRUPTION PHENOTYPE: Mice exhibit embryonic lethality.
CC {ECO:0000269|PubMed:34446558}.
CC -!- SIMILARITY: Belongs to the chemokine-like factor family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK94042.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK005577; BAB24133.1; -; mRNA.
DR EMBL; AK046397; BAC32704.1; -; mRNA.
DR EMBL; AK050112; BAC34072.1; -; mRNA.
DR EMBL; AK086063; BAC39602.1; -; mRNA.
DR EMBL; AK156237; BAE33637.1; -; mRNA.
DR EMBL; AK172265; BAE42913.1; -; mRNA.
DR EMBL; AF401530; AAK94042.1; ALT_SEQ; mRNA.
DR EMBL; AF401531; AAK94043.2; -; mRNA.
DR EMBL; AY046597; AAL05945.1; -; mRNA.
DR EMBL; AY047360; AAL10703.1; -; mRNA.
DR CCDS; CCDS40449.1; -. [Q9DAS1-2]
DR CCDS; CCDS40450.1; -. [Q9DAS1-1]
DR CCDS; CCDS85592.1; -. [Q9DAS1-4]
DR RefSeq; NP_001032930.1; NM_001037841.3. [Q9DAS1-2]
DR RefSeq; NP_001273312.1; NM_001286383.1. [Q9DAS1-4]
DR RefSeq; NP_083571.1; NM_029295.3. [Q9DAS1-1]
DR AlphaFoldDB; Q9DAS1; -.
DR SMR; Q9DAS1; -.
DR STRING; 10090.ENSMUSP00000034342; -.
DR PhosphoSitePlus; Q9DAS1; -.
DR PaxDb; Q9DAS1; -.
DR PRIDE; Q9DAS1; -.
DR ProteomicsDB; 283511; -. [Q9DAS1-1]
DR ProteomicsDB; 283513; -. [Q9DAS1-4]
DR Antibodypedia; 29261; 124 antibodies from 22 providers.
DR DNASU; 75458; -.
DR Ensembl; ENSMUST00000034342; ENSMUSP00000034342; ENSMUSG00000054400. [Q9DAS1-1]
DR Ensembl; ENSMUST00000098464; ENSMUSP00000096064; ENSMUSG00000054400. [Q9DAS1-2]
DR Ensembl; ENSMUST00000211809; ENSMUSP00000148697; ENSMUSG00000054400. [Q9DAS1-4]
DR Ensembl; ENSMUST00000212410; ENSMUSP00000148816; ENSMUSG00000054400. [Q9DAS1-5]
DR GeneID; 75458; -.
DR KEGG; mmu:75458; -.
DR UCSC; uc009nac.2; mouse. [Q9DAS1-4]
DR UCSC; uc009nad.2; mouse. [Q9DAS1-1]
DR UCSC; uc009nag.2; mouse. [Q9DAS1-2]
DR CTD; 51192; -.
DR MGI; MGI:1922708; Cklf.
DR VEuPathDB; HostDB:ENSMUSG00000054400; -.
DR eggNOG; KOG4788; Eukaryota.
DR GeneTree; ENSGT00940000162264; -.
DR HOGENOM; CLU_108546_3_0_1; -.
DR InParanoid; Q9DAS1; -.
DR OMA; CCSVKGH; -.
DR OrthoDB; 1423020at2759; -.
DR PhylomeDB; Q9DAS1; -.
DR TreeFam; TF317387; -.
DR BioGRID-ORCS; 75458; 3 hits in 75 CRISPR screens.
DR ChiTaRS; Cklf; mouse.
DR PRO; PR:Q9DAS1; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9DAS1; protein.
DR Bgee; ENSMUSG00000054400; Expressed in spermatocyte and 201 other tissues.
DR ExpressionAtlas; Q9DAS1; baseline and differential.
DR Genevisible; Q9DAS1; MM.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0008009; F:chemokine activity; ISO:MGI.
DR GO; GO:0030595; P:leukocyte chemotaxis; ISO:MGI.
DR GO; GO:0048247; P:lymphocyte chemotaxis; ISO:MGI.
DR GO; GO:0048246; P:macrophage chemotaxis; ISO:MGI.
DR GO; GO:0030593; P:neutrophil chemotaxis; ISO:MGI.
DR GO; GO:0032940; P:secretion by cell; ISO:MGI.
DR InterPro; IPR008253; Marvel.
DR PROSITE; PS51225; MARVEL; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chemotaxis; Cytokine; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..152
FT /note="Chemokine-like factor"
FT /id="PRO_0000186095"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 13..133
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT VAR_SEQ 80..111
FT /note="Missing (in isoform CKLF4)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_050599"
FT VAR_SEQ 80..96
FT /note="DVINSMVTALCMLIVSV -> ISFPCECRLVSCQLDPS (in isoform
FT CKLF3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_050600"
FT VAR_SEQ 97..152
FT /note="Missing (in isoform CKLF3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_050602"
FT VAR_SEQ 112..152
FT /note="VFGFLTVTCTIADCALMCQKLRFRPRQPYQKKSTNDIDDRE -> QGPHRTD
FT LALP (in isoform CKLF5)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_050603"
SQ SEQUENCE 152 AA; 17254 MW; 4595E0E25513A587 CRC64;
METPRPVVSR RPFCCTLKCF VKFLRLVVTV TSMIFFIVGQ APEPYIVITG FEVTVIFCFL
VLYTCGLDKI MRSFFWPLLD VINSMVTALC MLIVSVLALI PETSTKTILG GVFGFLTVTC
TIADCALMCQ KLRFRPRQPY QKKSTNDIDD RE
