ID   CKN3_CONIM              Reviewed;          70 AA.
AC   D0PX86;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Conotoxin Im23.3 {ECO:0000303|PubMed:22399292};
DE   Flags: Precursor;
OS   Conus imperialis (Imperial cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Stephanoconus.
OX   NCBI_TaxID=35631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND NOMENCLATURE.
RC   TISSUE=Venom duct;
RX   PubMed=22399292; DOI=10.1074/jbc.m111.334615;
RA   Ye M., Khoo K.K., Xu S., Zhou M., Boonyalai N., Perugini M.A., Shao X.,
RA   Chi C., Galea C.A., Wang C., Norton R.S.;
RT   "A helical conotoxin from Conus imperialis has a novel cysteine framework
RT   and defines a new superfamily.";
RL   J. Biol. Chem. 287:14973-14983(2012).
CC   -!- FUNCTION: Neurotoxin that induces excitatory symptoms in mice following
CC       intracranial administration. No symptoms are observed after
CC       intraperitoneal and intravenous (tail vein) injections (By similarity).
CC       {ECO:0000250|UniProtKB:D0PX84}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:22399292}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:22399292}.
CC   -!- DOMAIN: The cysteine framework is XXIII (C-C-C-CC-C). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the conotoxin K superfamily. {ECO:0000305}.
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DR   EMBL; FJ375240; ACQ66000.1; -; mRNA.
DR   AlphaFoldDB; D0PX86; -.
DR   SMR; D0PX86; -.
DR   TCDB; 8.B.39.1.1; the conotoxin k (cotxj) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Cleavage on pair of basic residues; Disulfide bond; Neurotoxin; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..28
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000417045"
FT   CHAIN           31..70
FT                   /note="Conotoxin Im23.3"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000417128"
FT   DISULFID        34..41
FT                   /evidence="ECO:0000250|UniProtKB:D0PX84"
FT   DISULFID        45..53
FT                   /evidence="ECO:0000250|UniProtKB:D0PX84"
FT   DISULFID        54..69
FT                   /evidence="ECO:0000250|UniProtKB:D0PX84"
SQ   SEQUENCE   70 AA;  7825 MW;  78163B4ABF61B09E CRC64;
     MIMRMTLTLF VLVVMTAASA SGDALTEAKR IPYCGQTGAE CYSWCKEQHL IRCCDFVKYV
     GMNPPADKCR