CKP2L_BOVIN
ID CKP2L_BOVIN Reviewed; 744 AA.
AC A5PK21; Q0V8I3;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Cytoskeleton-associated protein 2-like;
DE AltName: Full=Radial fiber and mitotic spindle protein;
DE Short=Radmis;
GN Name=CKAP2L;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Thymus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 196-744.
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
CC -!- FUNCTION: Microtubule-associated protein required for mitotic spindle
CC formation and cell-cycle progression in neural progenitor cells.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250}. Note=Uniformly distributed along each microtubule bundle
CC of spindles in addition to centrioles during mitosis, expression
CC promptly diminishes at interphase. {ECO:0000250}.
CC -!- DOMAIN: The KEN box is required for the association with the APC/C-Cdh1
CC complex, ubiquitination and degradation. {ECO:0000250}.
CC -!- PTM: Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CKAP2 family. {ECO:0000305}.
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DR EMBL; BC142323; AAI42324.1; -; mRNA.
DR EMBL; BT026235; ABG67074.1; -; mRNA.
DR RefSeq; NP_001092371.1; NM_001098901.2.
DR AlphaFoldDB; A5PK21; -.
DR SMR; A5PK21; -.
DR STRING; 9913.ENSBTAP00000002964; -.
DR PaxDb; A5PK21; -.
DR PRIDE; A5PK21; -.
DR Ensembl; ENSBTAT00000002964; ENSBTAP00000002964; ENSBTAG00000002298.
DR GeneID; 507498; -.
DR KEGG; bta:507498; -.
DR CTD; 150468; -.
DR VEuPathDB; HostDB:ENSBTAG00000002298; -.
DR VGNC; VGNC:27381; CKAP2L.
DR eggNOG; ENOG502RZUT; Eukaryota.
DR GeneTree; ENSGT00530000063691; -.
DR HOGENOM; CLU_022701_0_0_1; -.
DR InParanoid; A5PK21; -.
DR OMA; QKSTQPC; -.
DR OrthoDB; 417608at2759; -.
DR TreeFam; TF333003; -.
DR Proteomes; UP000009136; Chromosome 11.
DR Bgee; ENSBTAG00000002298; Expressed in semen and 97 other tissues.
DR ExpressionAtlas; A5PK21; baseline and differential.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR InterPro; IPR029197; CKAP2_C.
DR Pfam; PF15297; CKAP2_C; 2.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoskeleton; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..744
FT /note="Cytoskeleton-associated protein 2-like"
FT /id="PRO_0000324334"
FT REGION 35..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 76..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 182..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 317..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 428..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..216
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..336
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 744
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IYA6"
FT CROSSLNK 195
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q8IYA6"
FT CROSSLNK 195
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q8IYA6"
SQ SEQUENCE 744 AA; 82608 MW; 3751BB248DBFC036 CRC64;
MVRPGLSAAA EERQRKLQEY LAAKGKLKCQ NTKPYLKAKN NCPNPPHSKS TIGPKKDVTN
HVALPVKTTR SINIKLQSRP ANITRSQRPK LEPPKLGKRL TSESVSSNPN GKPPGNSQQL
RGFGSSTDGK QPRKTMGSLN VQKLKTTKQQ VTDQRTAKGT DPVDNTHVEN ESLGGCLKEM
NKENLPQDLP NSERKPNPES WTINKPQTNQ TKSSLASTRE VLDKSSVNSA ALKEGVIKPF
VEETQISVPP GKSQKLSRVA DLIRPGGKPP KTLPSHFVQT LNRTQATKKT VVKDIKSIKV
NRSKYERPNE AKLQSYTVTE QKVKHSKPST HPSVLQGGCN HRHPNIKQDH KPTQACCRPQ
TSYALQKSKA ISQRPNLTVG SFNSVIPSTP SIRANGATGN KCNNSCQQRA RTLDSKFKSA
PPQKCFLNKT APRTQAGGPT ISGRGVPNGA QTNPCKKIAA EDRRKQLEEW RKSKGKIYKR
PPMELKTKRK IIEEMNISFW KSMEKEEEEK KAQLELSNKI NNTLTECLQL IERGVLSNEV
FAILSSIPEA EKFAKFWICK AKLLASKGTF DVIGLYEEAI RNGATPIQEL REVVLNILQD
RNRTTEGMTS NSLVAETNIT SIEELAKKTE SGASCLSPKE SEQMSVTPQI TKSEQDGHPG
IKLQIAPIPR INGMPEVQDM KLITPVRRSA RIERAVSRYP EMLQEHDLVV ASLNELLEVE
ETECFIFRKN EALPVTLGFP VSES
