CKP2L_MOUSE
ID CKP2L_MOUSE Reviewed; 745 AA.
AC Q7TS74; Q3U5A3; Q8K264; U6C6D6;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Cytoskeleton-associated protein 2-like;
DE AltName: Full=Radial fiber and mitotic spindle protein;
DE Short=Radmis;
GN Name=Ckap2l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 590-745 (ISOFORM 1), FUNCTION, SUBCELLULAR
RP LOCATION, MUTAGENESIS OF 183-LYS--ARG-185, KEN BOX MOTIF, UBIQUITINATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=24260314; DOI=10.1371/journal.pone.0079895;
RA Yumoto T., Nakadate K., Nakamura Y., Sugitani Y., Sugitani-Yoshida R.,
RA Ueda S., Sakakibara S.;
RT "Radmis, a novel mitotic spindle protein that functions in cell division of
RT neural progenitors.";
RL PLoS ONE 8:E79895-E79895(2013).
RN [5]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=25439729; DOI=10.1016/j.ajhg.2014.10.008;
RA Hussain M.S., Battaglia A., Szczepanski S., Kaygusuz E., Toliat M.R.,
RA Sakakibara S., Altmueller J., Thiele H., Nuernberg G., Moosa S., Yigit G.,
RA Beleggia F., Tinschert S., Clayton-Smith J., Vasudevan P., Urquhart J.E.,
RA Donnai D., Fryer A., Percin F., Brancati F., Dobbie A., Smigiel R.,
RA Gillessen-Kaesbach G., Wollnik B., Noegel A.A., Newman W.G., Nuernberg P.;
RT "Mutations in CKAP2L, the human homolog of the mouse Radmis gene, cause
RT Filippi syndrome.";
RL Am. J. Hum. Genet. 95:622-632(2014).
CC -!- FUNCTION: Microtubule-associated protein required for mitotic spindle
CC formation and cell-cycle progression in neural progenitor cells.
CC {ECO:0000269|PubMed:24260314}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000269|PubMed:24260314, ECO:0000269|PubMed:25439729}.
CC Note=Uniformly distributed along each microtubule bundle of spindles in
CC addition to centrioles during mitosis, expression promptly diminishes
CC at interphase.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7TS74-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TS74-2; Sequence=VSP_032221, VSP_032222;
CC -!- TISSUE SPECIFICITY: Highly expressed in regions of active neurogenesis
CC and neural stem/progenitor cells (NSPCs), both embryonic and adult, not
CC detected in lung, liver, kidney, heart, and skeletal muscle.
CC {ECO:0000269|PubMed:24260314}.
CC -!- DEVELOPMENTAL STAGE: At 10.5 dpc, strongly expressed in the neural
CC progenitor cells throughout the neural tube and in the myotome.
CC Expression is significantly lower in connective tissues compared to
CC neural tube, but can be detected at the mitotic spindles of dividing
CC mesenchymal cells loosely distributed in the developing limb bud. In an
CC 12.5 dpc forelimb bud, detected in the dividing cells at the boundary
CC region between protruding cartilage and surrounding mesenchyme (at
CC protein level). {ECO:0000269|PubMed:25439729}.
CC -!- DOMAIN: The KEN box is required for the association with the APC/C-Cdh1
CC complex, ubiquitination and degradation. {ECO:0000269|PubMed:24260314}.
CC -!- PTM: Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
CC {ECO:0000269|PubMed:24260314}.
CC -!- SIMILARITY: Belongs to the CKAP2 family. {ECO:0000305}.
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DR EMBL; AK153766; BAE32177.1; -; mRNA.
DR EMBL; AL772347; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC032969; AAH32969.1; -; mRNA.
DR EMBL; BC053443; AAH53443.1; -; mRNA.
DR EMBL; AB455263; BAO05265.1; -; mRNA.
DR CCDS; CCDS16724.1; -. [Q7TS74-1]
DR RefSeq; NP_853620.1; NM_181589.3. [Q7TS74-1]
DR AlphaFoldDB; Q7TS74; -.
DR SMR; Q7TS74; -.
DR BioGRID; 214073; 1.
DR STRING; 10090.ENSMUSP00000056145; -.
DR iPTMnet; Q7TS74; -.
DR PhosphoSitePlus; Q7TS74; -.
DR EPD; Q7TS74; -.
DR MaxQB; Q7TS74; -.
DR PaxDb; Q7TS74; -.
DR PeptideAtlas; Q7TS74; -.
DR PRIDE; Q7TS74; -.
DR ProteomicsDB; 283515; -. [Q7TS74-1]
DR ProteomicsDB; 283516; -. [Q7TS74-2]
DR Antibodypedia; 33290; 75 antibodies from 16 providers.
DR DNASU; 70466; -.
DR Ensembl; ENSMUST00000052708; ENSMUSP00000056145; ENSMUSG00000048327. [Q7TS74-1]
DR GeneID; 70466; -.
DR KEGG; mmu:70466; -.
DR UCSC; uc008mho.2; mouse. [Q7TS74-1]
DR UCSC; uc008mhp.2; mouse. [Q7TS74-2]
DR CTD; 150468; -.
DR MGI; MGI:1917716; Ckap2l.
DR VEuPathDB; HostDB:ENSMUSG00000048327; -.
DR eggNOG; ENOG502RZUT; Eukaryota.
DR GeneTree; ENSGT00530000063691; -.
DR HOGENOM; CLU_022701_0_0_1; -.
DR InParanoid; Q7TS74; -.
DR OMA; QKSTQPC; -.
DR OrthoDB; 417608at2759; -.
DR PhylomeDB; Q7TS74; -.
DR TreeFam; TF333003; -.
DR BioGRID-ORCS; 70466; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Ckap2l; mouse.
DR PRO; PR:Q7TS74; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q7TS74; protein.
DR Bgee; ENSMUSG00000048327; Expressed in ear vesicle and 165 other tissues.
DR Genevisible; Q7TS74; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISO:MGI.
DR GO; GO:0072686; C:mitotic spindle; ISO:MGI.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR InterPro; IPR029197; CKAP2_C.
DR Pfam; PF15297; CKAP2_C; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Isopeptide bond;
KW Phosphoprotein; Reference proteome; Ubl conjugation.
FT CHAIN 1..745
FT /note="Cytoskeleton-associated protein 2-like"
FT /id="PRO_0000324336"
FT REGION 26..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 319..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 608..638
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 183..185
FT /note="KEN box"
FT COMPBIAS 63..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..168
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 429..451
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..479
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 608..625
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 745
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IYA6"
FT CROSSLNK 195
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q8IYA6"
FT CROSSLNK 195
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q8IYA6"
FT VAR_SEQ 589..619
FT /note="PVQELQEVLNVLQDPCRSTEAVTSDTSAAGT -> VSICLRWLMSNSPLWAS
FT FVAYSYGSTLHC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032221"
FT VAR_SEQ 620..745
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032222"
FT MUTAGEN 183..185
FT /note="KEN->AAA: Drastically enhances protein stability."
FT /evidence="ECO:0000269|PubMed:24260314"
FT CONFLICT 456
FT /note="P -> Q (in Ref. 1; BAE32177)"
FT /evidence="ECO:0000305"
FT CONFLICT 634
FT /note="Q -> H (in Ref. 4; BAO05265)"
FT /evidence="ECO:0000305"
FT CONFLICT 649..651
FT /note="APR -> PQ (in Ref. 3; AAH32969)"
FT /evidence="ECO:0000305"
FT CONFLICT 660
FT /note="H -> P (in Ref. 3; AAH32969)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 745 AA; 82999 MW; 0C1C922EB3850273 CRC64;
MVGPGPTASA AAEERWQKLQ EYLAAKGKLK DPNAKPYLKA KNICPKPPPS KYTPGPKKDV
SNHVLPSKTT RPINIKFQTK PASITASQKP ESKPPKLPSR GLTSRCFSSN TDCKQSSKPQ
QQPRAVSFTA GLSRNPRQCP DIQELKTKQQ QQAHGGNAKC THPETNTHAA KQPVDGFPDE
TNKENLPQAL PKPEKPDPEL HSIRKPNTGS SNQTQKGLAP KQILSKSSVT QTALKDRANK
QFIRNTQIRT QAVKSRPRPT VADSTRPREK PPQTAPSHSV PAHNKTQTSK KPMTKNTQDI
TVNRVRYGKP NETKIESCPA TEQKVKHTKP SSQLNVLQGG HNSRHPNMRQ DQKPVQPHLG
PQTSCVLQKS RAISQRPNLT ARNFNSVIPS TPNMRANKTL NNKYNNIFQQ KAQTLDSKFR
KFPPQSHFLN KTAPRTQAST AAASRKGAPS ATQTHPHGKK PEGEDRRKQL EEWQKSKGKT
YKRPPMKFKT KRKVIEEMNT SFWKSIEREE EEKKAQLELS KKIDSTLTEC LRLIEEGVLP
NEIFTIVSSI PEAEKFAKFW VCKAKLLASK GTFDAIGLYE EAIQNGATPV QELQEVLNVL
QDPCRSTEAV TSDTSAAGTN TTSAEELAKE ESEQPCPSLT EMEPIAAAAP RIPVSEWDNH
GIKLQVAPIP RICGMPEVQD MKLITPVRRS ARIERTVARY PEMLQEHDVV VASLNELLEV
DKTECFIFRE NEALPVTLGF EVLES
