CKS1_ARATH
ID CKS1_ARATH Reviewed; 87 AA.
AC O23249;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=Cyclin-dependent kinases regulatory subunit 1;
DE AltName: Full=CKS1-At;
GN Name=CKS1; OrderedLocusNames=At2g27960; ORFNames=T1E2.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH CDKA-1 AND CDKB1-1.
RX PubMed=9276444; DOI=10.1016/s0014-5793(97)00822-3;
RA de Veylder L., Segers G., Glab N., Casteels P., van Montagu M., Inze D.;
RT "The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt
RT and Cdc2bAt.";
RL FEBS Lett. 412:446-452(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH CDKA-1.
RX PubMed=10100639; DOI=10.1016/s0014-5793(99)00211-2;
RA Porceddu A., de Veylder L., Hayles J., van Montagu M., Inze D., Mironov V.;
RT "Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases
RT in fission yeast.";
RL FEBS Lett. 446:182-188(1999).
RN [8]
RP INTERACTION WITH CDKB1-2 AND CDKB2-1.
RX PubMed=11432958; DOI=10.1093/jxb/52.359.1381;
RA Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.;
RT "Identification of novel cyclin-dependent kinases interacting with the CKS1
RT protein of Arabidopsis.";
RL J. Exp. Bot. 52:1381-1382(2001).
RN [9]
RP FUNCTION, INTERACTION WITH CDKA-1 AND CDKB1-1, AND MUTAGENESIS OF GLU-61.
RX PubMed=11319029; DOI=10.1046/j.1365-313x.2001.00996.x;
RA de Veylder L., Beemster G.T.S., Beeckman T., Inze D.;
RT "CKS1At overexpression in Arabidopsis thaliana inhibits growth by reducing
RT meristem size and inhibiting cell-cycle progression.";
RL Plant J. 25:617-626(2001).
RN [10]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11971144; DOI=10.1105/tpc.010445;
RA Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.;
RT "Genome-wide analysis of core cell cycle genes in Arabidopsis.";
RL Plant Cell 14:903-916(2002).
RN [11]
RP INTERACTION WITH CDKA-1; CYCD2-1 AND AT4G14310.
RX PubMed=20706207; DOI=10.1038/msb.2010.53;
RA Van Leene J., Hollunder J., Eeckhout D., Persiau G., Van De Slijke E.,
RA Stals H., Van Isterdael G., Verkest A., Neirynck S., Buffel Y., De Bodt S.,
RA Maere S., Laukens K., Pharazyn A., Ferreira P.C.G., Eloy N., Renne C.,
RA Meyer C., Faure J.-D., Steinbrenner J., Beynon J., Larkin J.C.,
RA Van de Peer Y., Hilson P., Kuiper M., De Veylder L., Van Onckelen H.,
RA Inze D., Witters E., De Jaeger G.;
RT "Targeted interactomics reveals a complex core cell cycle machinery in
RT Arabidopsis thaliana.";
RL Mol. Syst. Biol. 6:397-397(2010).
CC -!- FUNCTION: Associates with cyclin-dependent kinases (CDKs) and plays an
CC essential role in the regulation of the cell cycle that affects plant
CC growth rate. May inhibit both the G1/S and G2/M phases.
CC {ECO:0000269|PubMed:11319029}.
CC -!- SUBUNIT: Interacts with CDKA-1 (PubMed:10100639, PubMed:11319029,
CC PubMed:9276444, PubMed:20706207). Interacts with CDKB1-1, CDKB1-2 and
CC CDKB2-1 (PubMed:11319029, PubMed:9276444, PubMed:11432958). Interacts
CC with CYCD2-1 and At4g14310 (PubMed:20706207).
CC {ECO:0000269|PubMed:10100639, ECO:0000269|PubMed:11319029,
CC ECO:0000269|PubMed:11432958, ECO:0000269|PubMed:20706207,
CC ECO:0000269|PubMed:9276444}.
CC -!- INTERACTION:
CC O23249; P24100: CDKA-1; NbExp=9; IntAct=EBI-1253127, EBI-371713;
CC O23249; P25859: CDKB1-1; NbExp=7; IntAct=EBI-1253127, EBI-1253311;
CC O23249; Q8LF80: CDKB2-1; NbExp=5; IntAct=EBI-1253127, EBI-1253579;
CC -!- MISCELLANEOUS: Plants overexpressing CKS1 show reduced leaf size and
CC root growth rates resulting from an increase of the cell-cycle duration
CC with an equal extension of both G1 and G2 phases and a shortening of
CC the meristem.
CC -!- SIMILARITY: Belongs to the CKS family. {ECO:0000305}.
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DR EMBL; AJ000016; CAA03859.1; -; mRNA.
DR EMBL; AC006929; AAD21506.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08064.1; -; Genomic_DNA.
DR EMBL; AK175719; BAD43482.1; -; mRNA.
DR EMBL; AK175828; BAD43591.1; -; mRNA.
DR EMBL; AK176119; BAD43882.1; -; mRNA.
DR EMBL; BT024528; ABD38867.1; -; mRNA.
DR EMBL; AY088339; AAM65878.1; -; mRNA.
DR PIR; A84679; A84679.
DR RefSeq; NP_180363.1; NM_128355.3.
DR AlphaFoldDB; O23249; -.
DR SMR; O23249; -.
DR BioGRID; 2690; 51.
DR IntAct; O23249; 40.
DR STRING; 3702.AT2G27960.1; -.
DR MetOSite; O23249; -.
DR PaxDb; O23249; -.
DR PRIDE; O23249; -.
DR ProteomicsDB; 222611; -.
DR DNASU; 817340; -.
DR EnsemblPlants; AT2G27960.1; AT2G27960.1; AT2G27960.
DR GeneID; 817340; -.
DR Gramene; AT2G27960.1; AT2G27960.1; AT2G27960.
DR KEGG; ath:AT2G27960; -.
DR Araport; AT2G27960; -.
DR TAIR; locus:2057836; AT2G27960.
DR eggNOG; KOG3484; Eukaryota.
DR HOGENOM; CLU_140546_2_0_1; -.
DR InParanoid; O23249; -.
DR OMA; RPINYGQ; -.
DR OrthoDB; 1377855at2759; -.
DR PhylomeDB; O23249; -.
DR PRO; PR:O23249; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O23249; baseline and differential.
DR Genevisible; O23249; AT.
DR GO; GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005777; C:peroxisome; HDA:TAIR.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0061575; F:cyclin-dependent protein serine/threonine kinase activator activity; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0042023; P:DNA endoreduplication; IDA:TAIR.
DR GO; GO:0000278; P:mitotic cell cycle; IDA:TAIR.
DR GO; GO:0051726; P:regulation of cell cycle; IMP:TAIR.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IBA:GO_Central.
DR Gene3D; 3.30.170.10; -; 1.
DR InterPro; IPR000789; Cyclin-dep_kinase_reg-sub.
DR InterPro; IPR036858; Cyclin-dep_kinase_reg-sub_sf.
DR Pfam; PF01111; CKS; 1.
DR PRINTS; PR00296; CYCLINKINASE.
DR SMART; SM01084; CKS; 1.
DR SUPFAM; SSF55637; SSF55637; 1.
DR PROSITE; PS00944; CKS_1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Reference proteome.
FT CHAIN 1..87
FT /note="Cyclin-dependent kinases regulatory subunit 1"
FT /id="PRO_0000294094"
FT MUTAGEN 61
FT /note="E->Q: Loss of function and impaired interaction with
FT CDKA-1 or CDKB1-1."
FT /evidence="ECO:0000269|PubMed:11319029"
SQ SEQUENCE 87 AA; 10521 MW; C8C58DEBDD7A095D CRC64;
MGQIQYSEKY FDDTFEYRHV VLPPEVAKLL PKNRLLSENE WRAIGVQQSR GWVHYAVHRP
EPHIMLFRRP LNYQQQQENQ AQNMLVK
