ID   CKX_WHEAT               Reviewed;          15 AA.
AC   P58763;
DT   03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2002, sequence version 1.
DT   11-DEC-2019, entry version 48.
DE   RecName: Full=Cytokinin dehydrogenase;
DE            EC=1.5.99.12;
DE   AltName: Full=Cytokinin oxidase;
DE            Short=CKO;
DE            Short=CKX;
DE   Flags: Fragment;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   PROTEIN SEQUENCE, AND CHARACTERIZATION.
RC   STRAIN=cv. Samantha;
RX   PubMed=11168382; DOI=10.1046/j.1432-1033.2001.01910.x;
RA   Galuszka P., Frebort I., Sebela M., Sauer P., Jacobsen S., Pec P.;
RT   "Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin degradation in
RT   cereals.";
RL   Eur. J. Biochem. 268:450-461(2001).
CC   -!- FUNCTION: Catalyzes the oxidation of cytokinins, a family of N(6)-
CC       substituted adenine derivatives that are plant hormones, where the
CC       substituent is an isopentenyl group. Substrate preference is 2-(2-
CC       hydroxyethylamino)-9-methyl-N(6)-isopentenyladenine >>
CC       isopentenyladenine > cis-zeatin = isopentenyladenosine = zeatin >>
CC       zeatin riboside.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + H2O + N(6)-dimethylallyladenine = 3-methyl-2-butenal +
CC         adenine + AH2; Xref=Rhea:RHEA:13625, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15825, ChEBI:CHEBI:16708,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:17660; EC=1.5.99.12;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 6.5.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Membrane. Note=Might be located on membranes.
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DR   Proteomes; UP000019116; Unplaced.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019139; F:cytokinin dehydrogenase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; FAD; Flavoprotein; Membrane; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..>15
FT                   /note="Cytokinin dehydrogenase"
FT                   /id="PRO_0000089839"
FT   UNSURE          1
FT   UNSURE          13..15
FT   NON_TER         15
SQ   SEQUENCE   15 AA;  1709 MW;  85B589BD53FCEDEF CRC64;
     FLPKSLFTLV TDKSL