ACHB3_HUMAN
ID ACHB3_HUMAN Reviewed; 458 AA.
AC Q05901; Q15827;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 199.
DE RecName: Full=Neuronal acetylcholine receptor subunit beta-3;
DE Flags: Precursor;
GN Name=CHRNB3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Substantia nigra;
RX PubMed=8906617; DOI=10.1007/bf02736842;
RA Elliott K.J., Ellis S.B., Berckhan K.J., Urrutia A., Chavez-Noriega L.E.,
RA Johnson E.C., Velicelebi G., Harpold M.M.;
RT "Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4
RT nicotinic acetylcholine receptor subunits and functional expression of the
RT alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits.";
RL J. Mol. Neurosci. 7:217-228(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pons;
RX PubMed=9009220; DOI=10.1016/s0014-5793(96)01383-x;
RA Groot Kormelink P.J., Luyten W.H.M.L.;
RT "Cloning and sequence of full-length cDNAs encoding the human neuronal
RT nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and
RT expression of seven nAChR subunits in the human neuroblastoma cell line SH-
RT SY5Y and/or IMR-32.";
RL FEBS Lett. 400:309-314(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Keddache M., Durner M., Greenberg D.A.;
RT "Genomic structure and mutation analysis of the CHRNB3 gene.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 36-458.
RC TISSUE=Brain stem;
RX PubMed=7690916; DOI=10.1016/0304-3940(93)90691-d;
RA Willoughby J.J., Ninkina N.N., Beech M.M., Latchman D.S., Wood J.N.;
RT "Molecular cloning of a human neuronal nicotinic acetylcholine receptor
RT beta 3-like subunit.";
RL Neurosci. Lett. 155:136-139(1993).
CC -!- FUNCTION: After binding acetylcholine, the AChR responds by an
CC extensive change in conformation that affects all subunits and leads to
CC opening of an ion-conducting channel across the plasma membrane.
CC -!- SUBUNIT: Neuronal AChR seems to be composed of two different type of
CC subunits: alpha and beta.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-3/CHRNB3 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; U62438; AAB40116.1; -; mRNA.
DR EMBL; Y08417; CAA69694.1; -; mRNA.
DR EMBL; AF140765; AAD33063.1; -; Genomic_DNA.
DR EMBL; AF140760; AAD33063.1; JOINED; Genomic_DNA.
DR EMBL; AF140761; AAD33063.1; JOINED; Genomic_DNA.
DR EMBL; AF140762; AAD33063.1; JOINED; Genomic_DNA.
DR EMBL; AF140763; AAD33063.1; JOINED; Genomic_DNA.
DR EMBL; AF140764; AAD33063.1; JOINED; Genomic_DNA.
DR EMBL; BC069681; AAH69681.1; -; mRNA.
DR EMBL; BC069703; AAH69703.1; -; mRNA.
DR EMBL; BC069788; AAH69788.1; -; mRNA.
DR EMBL; X67513; CAA47851.1; -; mRNA.
DR CCDS; CCDS6134.1; -.
DR PIR; I38056; I38056.
DR RefSeq; NP_000740.1; NM_000749.4.
DR AlphaFoldDB; Q05901; -.
DR SMR; Q05901; -.
DR BioGRID; 107564; 7.
DR ComplexPortal; CPX-2192; Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3.
DR IntAct; Q05901; 3.
DR STRING; 9606.ENSP00000289957; -.
DR BindingDB; Q05901; -.
DR ChEMBL; CHEMBL2109233; -.
DR DrugBank; DB00237; Butabarbital.
DR DrugBank; DB00898; Ethanol.
DR DrugBank; DB00184; Nicotine.
DR DrugBank; DB00202; Succinylcholine.
DR DrugCentral; Q05901; -.
DR GlyGen; Q05901; 3 sites.
DR PhosphoSitePlus; Q05901; -.
DR BioMuta; CHRNB3; -.
DR DMDM; 2506128; -.
DR MassIVE; Q05901; -.
DR MaxQB; Q05901; -.
DR PaxDb; Q05901; -.
DR PeptideAtlas; Q05901; -.
DR PRIDE; Q05901; -.
DR ProteomicsDB; 58355; -.
DR Antibodypedia; 11480; 185 antibodies from 33 providers.
DR DNASU; 1142; -.
DR Ensembl; ENST00000289957.3; ENSP00000289957.2; ENSG00000147432.7.
DR GeneID; 1142; -.
DR KEGG; hsa:1142; -.
DR MANE-Select; ENST00000289957.3; ENSP00000289957.2; NM_000749.5; NP_000740.1.
DR UCSC; uc003xpi.2; human.
DR CTD; 1142; -.
DR DisGeNET; 1142; -.
DR GeneCards; CHRNB3; -.
DR HGNC; HGNC:1963; CHRNB3.
DR HPA; ENSG00000147432; Tissue enhanced (brain, retina).
DR MIM; 118508; gene.
DR neXtProt; NX_Q05901; -.
DR OpenTargets; ENSG00000147432; -.
DR PharmGKB; PA26495; -.
DR VEuPathDB; HostDB:ENSG00000147432; -.
DR eggNOG; KOG3645; Eukaryota.
DR GeneTree; ENSGT00940000156892; -.
DR HOGENOM; CLU_018074_1_0_1; -.
DR InParanoid; Q05901; -.
DR OMA; QHSNDTI; -.
DR OrthoDB; 381858at2759; -.
DR PhylomeDB; Q05901; -.
DR TreeFam; TF315605; -.
DR PathwayCommons; Q05901; -.
DR Reactome; R-HSA-629594; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
DR Reactome; R-HSA-629597; Highly calcium permeable nicotinic acetylcholine receptors.
DR SignaLink; Q05901; -.
DR SIGNOR; Q05901; -.
DR BioGRID-ORCS; 1142; 6 hits in 1069 CRISPR screens.
DR ChiTaRS; CHRNB3; human.
DR GeneWiki; CHRNB3; -.
DR GenomeRNAi; 1142; -.
DR Pharos; Q05901; Tchem.
DR PRO; PR:Q05901; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q05901; protein.
DR Bgee; ENSG00000147432; Expressed in pancreatic ductal cell and 57 other tissues.
DR ExpressionAtlas; Q05901; baseline and differential.
DR Genevisible; Q05901; HS.
DR GO; GO:0005892; C:acetylcholine-gated channel complex; TAS:ProtInc.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0098691; C:dopaminergic synapse; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0042166; F:acetylcholine binding; IEA:Ensembl.
DR GO; GO:0022848; F:acetylcholine-gated cation-selective channel activity; IBA:GO_Central.
DR GO; GO:0015267; F:channel activity; TAS:ProtInc.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:1901363; F:heterocyclic compound binding; IEA:Ensembl.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; IEA:Ensembl.
DR GO; GO:0035094; P:response to nicotine; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0007271; P:synaptic transmission, cholinergic; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 2.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 2.
DR PRINTS; PR00254; NICOTINICR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..458
FT /note="Neuronal acetylcholine receptor subunit beta-3"
FT /id="PRO_0000000383"
FT TOPO_DOM 25..232
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..428
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 138
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 166
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 153..167
FT /evidence="ECO:0000250"
FT VARIANT 451
FT /note="K -> E (in dbSNP:rs35327613)"
FT /id="VAR_048173"
FT CONFLICT 36..38
FT /note="LFQ -> EWK (in Ref. 5; CAA47851)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 458 AA; 52729 MW; D6E919E53CBD21F8 CRC64;
MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS NDTIKVYFGL
KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI HSIKVPSESL WLPDIVLFEN
ADGRFEGSLM TKVIVKSNGT VVWTPPASYK SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM
VDLILINENV DRKDFFDNGE WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL
IIPCLGLSFL TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL
LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK DHVDRYSSPE
KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR YISRHVKKEH FISQVVQDWK
FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL KMWLHSYH
