ID   CLAT_DANRE              Reviewed;         637 AA.
AC   B2ZGJ1;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Choline O-acetyltransferase;
DE            Short=CHOACTase;
DE            Short=ChAT;
DE            Short=Choline acetylase;
DE            EC=2.3.1.6;
GN   Name=chat;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=18684905; DOI=10.1152/jn.90517.2008;
RA   Wang M., Wen H., Brehm P.;
RT   "Function of neuromuscular synapses in the zebrafish choline-
RT   acetyltransferase mutant bajan.";
RL   J. Neurophysiol. 100:1995-2004(2008).
CC   -!- FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh)
CC       from acetyl CoA and choline at cholinergic synapses.
CC       {ECO:0000269|PubMed:18684905}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + choline = acetylcholine + CoA;
CC         Xref=Rhea:RHEA:18821, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.6;
CC   -!- SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; EU660883; ACD40042.1; -; mRNA.
DR   RefSeq; NP_001124191.1; NM_001130719.1.
DR   RefSeq; XP_005156786.1; XM_005156729.3.
DR   AlphaFoldDB; B2ZGJ1; -.
DR   SMR; B2ZGJ1; -.
DR   STRING; 7955.ENSDARP00000125038; -.
DR   PaxDb; B2ZGJ1; -.
DR   Ensembl; ENSDART00000024225; ENSDARP00000025508; ENSDARG00000015854.
DR   Ensembl; ENSDART00000150228; ENSDARP00000125038; ENSDARG00000015854.
DR   GeneID; 100170938; -.
DR   KEGG; dre:100170938; -.
DR   CTD; 100170938; -.
DR   ZFIN; ZDB-GENE-080102-2; chata.
DR   eggNOG; KOG3717; Eukaryota.
DR   GeneTree; ENSGT01050000244830; -.
DR   HOGENOM; CLU_013513_3_0_1; -.
DR   InParanoid; B2ZGJ1; -.
DR   OMA; MAYRDPV; -.
DR   OrthoDB; 559299at2759; -.
DR   PhylomeDB; B2ZGJ1; -.
DR   TreeFam; TF313836; -.
DR   BRENDA; 2.3.1.6; 928.
DR   Reactome; R-DRE-1483191; Synthesis of PC.
DR   Reactome; R-DRE-264642; Acetylcholine Neurotransmitter Release Cycle.
DR   PRO; PR:B2ZGJ1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 13.
DR   Bgee; ENSDARG00000015854; Expressed in retina and 19 other tissues.
DR   ExpressionAtlas; B2ZGJ1; baseline.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0045202; C:synapse; IEA:GOC.
DR   GO; GO:0004102; F:choline O-acetyltransferase activity; IBA:GO_Central.
DR   GO; GO:0008292; P:acetylcholine biosynthetic process; IBA:GO_Central.
DR   GO; GO:0007626; P:locomotory behavior; IMP:UniProtKB.
DR   GO; GO:0007274; P:neuromuscular synaptic transmission; IBA:GO_Central.
DR   Gene3D; 3.30.559.10; -; 1.
DR   Gene3D; 3.30.559.70; -; 1.
DR   InterPro; IPR000542; Carn_acyl_trans.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR039551; Cho/carn_acyl_trans.
DR   InterPro; IPR042231; Cho/carn_acyl_trans_2.
DR   PANTHER; PTHR22589; PTHR22589; 1.
DR   Pfam; PF00755; Carn_acyltransf; 1.
DR   PROSITE; PS00439; ACYLTRANSF_C_1; 1.
DR   PROSITE; PS00440; ACYLTRANSF_C_2; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Neurotransmitter biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..637
FT                   /note="Choline O-acetyltransferase"
FT                   /id="PRO_0000351550"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        329
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         407..419
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   BINDING         445
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
FT   BINDING         545
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   637 AA;  71840 MW;  2379C23ADABC755A CRC64;
     MPVSKREQSK DTGDPCALPK LPIPPLKQTL DMYLTCMGHL VPEDQFRKTK AVVEKFGAPG
     GVGETLQKKL LERSEQKANW VYDYWLEDMY LNNRLALPVN SSPVMVFHKQ NFKGQSDVLR
     FAANLISGVL EYKALIDGRA LPVEHARGQL AGTPLCMDQY NKVFTSYRLP GTKTDTLVAQ
     KSTVMPEPEH IIVACKNQFF VLDVMVNFRR LNEKDLYTQL ERIRKMADIE EERQPPIGLL
     TSDGRTQWAE ARNILIKDST NRDSLDMIER CLCLVCLDEE TATELNDSNR ALLMLHGGGT
     DKNGGNRWYD KPMQFVIGAD GCCGVVCEHS PFEGIVLVQC SEYLLRYMRG SPSKLVRAAS
     MSELPAPRRL RWKCSPDIQT FLSASADRLQ KLVKNLDMNV HKFTGYGKEF IKRQKMSPDA
     YVQVALQFTF YRCHGRLVPT YESASIRRFQ EGRVDNIRSS TPEALAFVKA MASGSKITDA
     EKMELLWTAI KAQTNYTILA ITGMAIDNHL LGLREIAKEL KLEKPELFSD TTYATSIHFT
     LSTSQVPTTE EMFCCYGPVV PNGYGACYNP QTDHILFCVS SFRECAETSS DLFVKTLEGC
     LKEMQDLCRK CNTEVKPADS TQRMEGNPKV MKNGSKS