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CLB_ARATH
ID   CLB_ARATH               Reviewed;         510 AA.
AC   Q9LEX1; P92940;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Calcium-dependent lipid-binding protein {ECO:0000303|PubMed:21252258};
DE            Short=AtCLB {ECO:0000303|PubMed:21252258};
DE            Short=CaLB protein {ECO:0000303|PubMed:15238157};
DE   AltName: Full=Protein NTMC 2 TYPE 4;
DE            Short=NTMC2TYPE4;
GN   Name=CLB {ECO:0000303|PubMed:21252258}; Synonyms=NTMC2T4;
GN   OrderedLocusNames=At3g61050 {ECO:0000312|Araport:AT3G61050};
GN   ORFNames=T27I15.140 {ECO:0000312|EMBL:CAB94141.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 13-510.
RA   Kopka J., Pical C., Gray J.E., Mueller-Roeber B.;
RL   Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15238157; DOI=10.1186/1471-2164-5-43;
RA   Craxton M.A.;
RT   "Synaptotagmin gene content of the sequenced genomes.";
RL   BMC Genomics 5:43-43(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
RN   [8]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=21252258; DOI=10.1093/jxb/erq468;
RA   de Silva K., Laska B., Brown C., Sederoff H.W., Khodakovskaya M.;
RT   "Arabidopsis thaliana calcium-dependent lipid-binding protein (AtCLB): a
RT   novel repressor of abiotic stress response.";
RL   J. Exp. Bot. 62:2679-2689(2011).
RN   [9]
RP   INTERACTION WITH THE MICROBOTRYUM VIOLACEUM EFFECTOR MVLG_01732.
RX   PubMed=29165363; DOI=10.3390/ijms18112489;
RA   Kuppireddy V.S., Uversky V.N., Toh S.S., Tsai M.C., Beckerson W.C.,
RA   Cahill C., Carman B., Perlin M.H.;
RT   "Identification and initial characterization of the effectors of an anther
RT   smut fungus and potential host target proteins.";
RL   Int. J. Mol. Sci. 18:0-0(2017).
CC   -!- FUNCTION: May be involved in membrane trafficking (By similarity). Acts
CC       as a repressor of abiotic stress (e.g. drought and salt) responses by
CC       binding specifically to the promoter of THAS1 to regulate its
CC       transcription (PubMed:21252258). Binds to membrane lipid ceramides
CC       (PubMed:21252258). {ECO:0000250|UniProtKB:B6ETT4,
CC       ECO:0000269|PubMed:21252258}.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000250|UniProtKB:A0FGR8};
CC   -!- SUBUNIT: Interacts with the biotrophic pathogenic fungi Microbotryum
CC       violaceum effector MVLG_01732. {ECO:0000269|PubMed:29165363}.
CC   -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:21252258};
CC       Single-pass membrane protein {ECO:0000255}. Note=Localized in the
CC       nucleus membrane of root tips cells. {ECO:0000269|PubMed:21252258}.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in rosette leaves and flowers, to
CC       lower extent, in cauline leaves, roots and stems, and, at low levels,
CC       in siliques. {ECO:0000269|PubMed:21252258}.
CC   -!- DOMAIN: The SMP-LTD domain is a barrel-like domain that can bind
CC       various types of glycerophospholipids in its interior and mediate their
CC       transfer between two adjacent bilayers. {ECO:0000255|PROSITE-
CC       ProRule:PRU01194}.
CC   -!- DISRUPTION PHENOTYPE: Enhanced drought and salt tolerance and modified
CC       gravitropic response associated with an increased expression of THAS1
CC       (PubMed:21252258). Higher root lengths (PubMed:21252258).
CC       {ECO:0000269|PubMed:21252258}.
CC   -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA65416.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL358732; CAB94141.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE80144.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE80145.1; -; Genomic_DNA.
DR   EMBL; BT004564; AAO42810.1; -; mRNA.
DR   EMBL; AK227322; BAE99336.1; -; mRNA.
DR   EMBL; X96598; CAA65416.1; ALT_INIT; mRNA.
DR   PIR; T50526; T50526.
DR   RefSeq; NP_001030908.1; NM_001035831.2.
DR   RefSeq; NP_191664.1; NM_115969.3.
DR   AlphaFoldDB; Q9LEX1; -.
DR   SMR; Q9LEX1; -.
DR   STRING; 3702.AT3G61050.2; -.
DR   PaxDb; Q9LEX1; -.
DR   PRIDE; Q9LEX1; -.
DR   ProteomicsDB; 181262; -.
DR   EnsemblPlants; AT3G61050.1; AT3G61050.1; AT3G61050.
DR   EnsemblPlants; AT3G61050.2; AT3G61050.2; AT3G61050.
DR   GeneID; 825277; -.
DR   Gramene; AT3G61050.1; AT3G61050.1; AT3G61050.
DR   Gramene; AT3G61050.2; AT3G61050.2; AT3G61050.
DR   KEGG; ath:AT3G61050; -.
DR   Araport; AT3G61050; -.
DR   TAIR; locus:2100992; AT3G61050.
DR   eggNOG; KOG1012; Eukaryota.
DR   HOGENOM; CLU_042212_1_0_1; -.
DR   InParanoid; Q9LEX1; -.
DR   OMA; WSRMMTH; -.
DR   OrthoDB; 52746at2759; -.
DR   PhylomeDB; Q9LEX1; -.
DR   PRO; PR:Q9LEX1; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LEX1; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IDA:TAIR.
DR   GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR   GO; GO:0097001; F:ceramide binding; IDA:UniProtKB.
DR   GO; GO:0008289; F:lipid binding; IDA:TAIR.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR   GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:TAIR.
DR   GO; GO:0009958; P:positive gravitropism; IMP:TAIR.
DR   GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR   GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR   Gene3D; 2.60.40.150; -; 1.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR031069; CLB_plant.
DR   InterPro; IPR031468; SMP_LBD.
DR   InterPro; IPR045050; Synaptotagmin_plant.
DR   InterPro; IPR039010; Synaptotagmin_SMP.
DR   PANTHER; PTHR10774; PTHR10774; 1.
DR   PANTHER; PTHR10774:SF201; PTHR10774:SF201; 1.
DR   Pfam; PF00168; C2; 1.
DR   Pfam; PF17047; SMP_LBD; 1.
DR   PRINTS; PR00360; C2DOMAIN.
DR   SMART; SM00239; C2; 1.
DR   SUPFAM; SSF49562; SSF49562; 1.
DR   PROSITE; PS50004; C2; 1.
DR   PROSITE; PS51847; SMP; 1.
PE   1: Evidence at protein level;
KW   Calcium; Coiled coil; Lipid transport; Lipid-binding; Membrane;
KW   Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW   Stress response; Transcription; Transcription regulation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..510
FT                   /note="Calcium-dependent lipid-binding protein"
FT                   /id="PRO_0000450279"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          66..250
FT                   /note="SMP-LTD 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01194"
FT   DOMAIN          66..248
FT                   /note="SMP-LTD 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01194"
FT   DOMAIN          242..362
FT                   /note="C2 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   DOMAIN          246..364
FT                   /note="C2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   DOMAIN          461..500
FT                   /note="C2 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   REGION          226..488
FT                   /note="Phospholipid binding"
FT                   /evidence="ECO:0000250|UniProtKB:P48018"
FT   REGION          484..510
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          390..417
FT                   /evidence="ECO:0000255"
FT   BINDING         278
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         279
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         279
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         285
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         285
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         333
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         333
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         334
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         335
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         335
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         335
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         339
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   BINDING         340
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:A0FGR8"
FT   CONFLICT        118
FT                   /note="L -> S (in Ref. 5; CAA65416)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        245
FT                   /note="V -> F (in Ref. 5; CAA65416)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        301..304
FT                   /note="KAIE -> NAID (in Ref. 5; CAA65416)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   510 AA;  55095 MW;  5AA0254953E166F6 CRC64;
     MGLISGILFG IIFGVALMAG WSRMMTHRSS KRVAKAVDMK LLGSLSRDDL KKICGDNFPQ
     WISFPAFEQV KWLNKLLSKM WPYIAEAATM VIRDSVEPLL EDYRPPGITS LKFSKLTLGN
     VAPKIEGIRV QSFKEGQVTM DVDLRWGGDP NIVLGVTALV ASIPIQLKDL QVFTVARVIF
     QLADEIPCIS AVVVALLAEP KPRIDYTLKA VGGSLTAIPG LSDMIDDTVD TIVKDMLQWP
     HRIVVPIGGI PVDLSDLELK PQGKLIVTVV KATNLKNKEL IGKSDPYATI YIRPVFKYKT
     KAIENNLNPV WDQTFELIAE DKETQSLTVE VFDKDVGQDE RLGLVKLPLS SLEAGVTKEL
     ELNLLSSLDT LKVKDKKDRG SITLKVHYHE FNKEEQMAAL EDEKKIMEER KRLKEAGVIG
     STMDAVGMVG SGLGAGVGMV GTGIGTGVGL VGSGVSSGVG MVGSGFGAVG SGLSKAGRFM
     GRTITGQSSK RSGSSTPVNT VPENDGAKQQ
 
 
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