CLCB_ARATH
ID CLCB_ARATH Reviewed; 780 AA.
AC P92942;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Chloride channel protein CLC-b;
DE Short=AtCLC-b;
DE AltName: Full=CBS domain-containing protein CBSCLC7;
GN Name=CLC-B; Synonyms=CBSCLC7; OrderedLocusNames=At3g27170; ORFNames=MYF5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=8969232; DOI=10.1074/jbc.271.52.33632;
RA Hechenberger M., Schwappach B., Fischer W.N., Frommer W.B., Jentsch T.J.,
RA Steinmeyer K.;
RT "A family of putative chloride channels from Arabidopsis and functional
RT complementation of a yeast strain with a CLC gene disruption.";
RL J. Biol. Chem. 271:33632-33638(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19400948; DOI=10.1186/1471-2164-10-200;
RA Kushwaha H.R., Singh A.K., Sopory S.K., Singla-Pareek S.L., Pareek A.;
RT "Genome wide expression analysis of CBS domain containing proteins in
RT Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their
RT developmental and stress regulation.";
RL BMC Genomics 10:200-200(2009).
RN [6]
RP INTERACTION WITH PP2A5.
RX PubMed=27676158; DOI=10.1111/pce.12837;
RA Hu R., Zhu Y., Wei J., Chen J., Shi H., Shen G., Zhang H.;
RT "Overexpression of PP2A-C5 that encodes the catalytic subunit 5 of protein
RT phosphatase 2A in Arabidopsis confers better root and shoot development
RT under salt conditions.";
RL Plant Cell Environ. 40:150-164(2017).
CC -!- FUNCTION: Voltage-gated chloride channel.
CC -!- SUBUNIT: Homodimer (By similarity). Interacts with PP2A5
CC (PubMed:27676158). {ECO:0000250, ECO:0000269|PubMed:27676158}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Broadly expressed in the plant.
CC -!- SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family.
CC {ECO:0000305}.
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DR EMBL; Z71446; CAA96058.1; -; mRNA.
DR EMBL; AP001312; BAB01934.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77275.1; -; Genomic_DNA.
DR EMBL; AY062518; AAL32596.1; -; mRNA.
DR RefSeq; NP_189353.1; NM_113631.4.
DR AlphaFoldDB; P92942; -.
DR SMR; P92942; -.
DR BioGRID; 7666; 18.
DR IntAct; P92942; 14.
DR STRING; 3702.AT3G27170.1; -.
DR iPTMnet; P92942; -.
DR PaxDb; P92942; -.
DR PRIDE; P92942; -.
DR ProteomicsDB; 246710; -.
DR EnsemblPlants; AT3G27170.1; AT3G27170.1; AT3G27170.
DR GeneID; 822336; -.
DR Gramene; AT3G27170.1; AT3G27170.1; AT3G27170.
DR KEGG; ath:AT3G27170; -.
DR Araport; AT3G27170; -.
DR TAIR; locus:2095123; AT3G27170.
DR eggNOG; KOG0474; Eukaryota.
DR HOGENOM; CLU_003181_4_0_1; -.
DR InParanoid; P92942; -.
DR OMA; MMAILFR; -.
DR OrthoDB; 410280at2759; -.
DR PhylomeDB; P92942; -.
DR PRO; PR:P92942; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P92942; baseline and differential.
DR Genevisible; P92942; AT.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0009671; F:nitrate:proton symporter activity; IDA:TAIR.
DR GO; GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR014743; Cl-channel_core.
DR InterPro; IPR001807; Cl-channel_volt-gated.
DR InterPro; IPR002251; Cl_channel_pln.
DR Pfam; PF00571; CBS; 1.
DR Pfam; PF00654; Voltage_CLC; 1.
DR PRINTS; PR00762; CLCHANNEL.
DR PRINTS; PR01120; CLCHANNELPLT.
DR SMART; SM00116; CBS; 2.
DR SUPFAM; SSF54631; SSF54631; 1.
DR SUPFAM; SSF81340; SSF81340; 1.
DR PROSITE; PS51371; CBS; 2.
PE 1: Evidence at protein level;
KW CBS domain; Chloride; Chloride channel; Ion channel; Ion transport;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW Transport; Voltage-gated channel.
FT CHAIN 1..780
FT /note="Chloride channel protein CLC-b"
FT /id="PRO_0000094466"
FT TRANSMEM 87..107
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 509..529
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..550
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TRANSMEM 735..755
FT /note="Helical; Name=13"
FT /evidence="ECO:0000255"
FT DOMAIN 594..663
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 708..770
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 780 AA; 85458 MW; 0E9FB0FBF52AEAA5 CRC64;
MVEEDLNQIG GNSNYNGEGG DPESNTLNQP LVKANRTLSS TPLALVGAKV SHIESLDYEI
NENDLFKHDW RKRSKAQVLQ YVFLKWTLAC LVGLFTGLIA TLINLAVENI AGYKLLAVGH
FLTQERYVTG LMVLVGANLG LTLVASVLCV CFAPTAAGPG IPEIKAYLNG VDTPNMFGAT
TMIVKIVGSI GAVAAGLDLG KEGPLVHIGS CIASLLGQGG TDNHRIKWRW LRYFNNDRDR
RDLITCGSAA GVCAAFRSPV GGVLFALEEV ATWWRSALLW RTFFSTAVVV VVLREFIEIC
NSGKCGLFGK GGLIMFDVSH VTYTYHVTDI IPVMLIGVIG GILGSLYNHL LHKVLRLYNL
INEKGKIHKV LLSLTVSLFT SVCLYGLPFL AKCKPCDPSI DEICPTNGRS GNFKQFHCPK
GYYNDLATLL LTTNDDAVRN LFSSNTPNEF GMGSLWIFFV LYCILGLFTF GIATPSGLFL
PIILMGAAYG RMLGAAMGSY TSIDQGLYAV LGAAALMAGS MRMTVSLCVI FLELTNNLLL
LPITMIVLLI AKTVGDSFNP SIYDIILHLK GLPFLEANPE PWMRNLTVGE LGDAKPPVVT
LQGVEKVSNI VDVLKNTTHN AFPVLDEAEV PQVGLATGAT ELHGLILRAH LVKVLKKRWF
LTEKRRTEEW EVREKFPWDE LAEREDNFDD VAITSAEMEM YVDLHPLTNT TPYTVMENMS
VAKALVLFRQ VGLRHLLIVP KIQASGMCPV VGILTRQDLR AYNILQAFPL LEKSKGGKTH
