CLCB_ECOLI
ID CLCB_ECOLI Reviewed; 418 AA.
AC P76175; P77195;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Voltage-gated ClC-type chloride channel ClcB;
GN Name=clcB; Synonyms=mriT, ynfJ; OrderedLocusNames=b1592, JW5263;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12384697; DOI=10.1038/nature01000;
RA Iyer R., Iverson T.M., Accardi A., Miller C.;
RT "A biological role for prokaryotic ClC chloride channels.";
RL Nature 419:715-718(2002).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Probably acts as an electrical shunt for an outwardly-
CC directed proton pump that is linked to amino acid decarboxylation, as
CC part of the extreme acid resistance (XAR) response.
CC {ECO:0000269|PubMed:12384697}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family. ClcB
CC subfamily. {ECO:0000305}.
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DR EMBL; U00096; AAC74664.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15316.2; -; Genomic_DNA.
DR PIR; B64915; B64915.
DR RefSeq; NP_416109.2; NC_000913.3.
DR AlphaFoldDB; P76175; -.
DR SMR; P76175; -.
DR BioGRID; 4259118; 8.
DR DIP; DIP-28065N; -.
DR IntAct; P76175; 2.
DR STRING; 511145.b1592; -.
DR PaxDb; P76175; -.
DR PRIDE; P76175; -.
DR EnsemblBacteria; AAC74664; AAC74664; b1592.
DR EnsemblBacteria; BAA15316; BAA15316; BAA15316.
DR GeneID; 947179; -.
DR KEGG; ecj:JW5263; -.
DR KEGG; eco:b1592; -.
DR PATRIC; fig|1411691.4.peg.670; -.
DR EchoBASE; EB3609; -.
DR eggNOG; COG0038; Bacteria.
DR HOGENOM; CLU_015263_5_2_6; -.
DR InParanoid; P76175; -.
DR OMA; GPLTMTF; -.
DR PhylomeDB; P76175; -.
DR BioCyc; EcoCyc:YNFJ-MON; -.
DR BioCyc; MetaCyc:YNFJ-MON; -.
DR PRO; PR:P76175; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005247; F:voltage-gated chloride channel activity; IEA:UniProtKB-UniRule.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR GO; GO:0010447; P:response to acidic pH; IEA:InterPro.
DR HAMAP; MF_01203; CLC_ClcB; 1.
DR InterPro; IPR014743; Cl-channel_core.
DR InterPro; IPR001807; Cl-channel_volt-gated.
DR InterPro; IPR023790; Cl-channel_volt-gated_ClcB.
DR Pfam; PF00654; Voltage_CLC; 1.
DR PRINTS; PR00762; CLCHANNEL.
DR SUPFAM; SSF81340; SSF81340; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Chloride; Chloride channel;
KW Ion channel; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..418
FT /note="Voltage-gated ClC-type chloride channel ClcB"
FT /id="PRO_0000094485"
FT TOPO_DOM 1
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..53
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..177
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..257
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..315
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 337..351
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..379
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..418
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 418 AA; 44153 MW; F19EAFD7C87F4C01 CRC64;
MFHRLLIATV VGILAAFAVA GFRHAMLLLE WLFLNNDSGS LVNAATNLSP WRRLLTPALG
GLAAGLLLMG WQKFTQQRPH APTDYMEALQ TDGQFDYAAS LVKSLASLLV VTSGSAIGRE
GAMILLAALA ASCFAQRFTP RQEWKLWIAC GAAAGMAAAY RAPLAGSLFI AEVLFGTMML
ASLGPVIISA VVALLVSNLI NHSDALLYNV QLSVTVQARD YALIISTGVL AGLCGPLLLT
LMNACHRGFV SLKLAPPWQL ALGGLIVGLL SLFTPAVWGN GYSTVQSFLT APPLLMIIAG
IFLCKLCAVL ASSGSGAPGG VFTPTLFIGL AIGMLYGRSL GLWFPDGEEI TLLLGLTGMA
TLLAATTHAP IMSTLMICEM TGEYQLLPGL LIACVIASVI SRTLHRDSIY RQHTAQHS
