CLCC1_RAT
ID CLCC1_RAT Reviewed; 541 AA.
AC Q9WU61; Q66HQ5; Q8VIE8; Q8VIE9; Q8VIF0; Q8VIF1;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Chloride channel CLIC-like protein 1;
DE AltName: Full=Mid-1-related chloride channel protein 1;
DE Flags: Precursor;
GN Name=Clcc1; Synonyms=Mclc;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5), FUNCTION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=11279057; DOI=10.1074/jbc.m100366200;
RA Nagasawa M., Kanzaki M., Iino Y., Morishita Y., Kojima I.;
RT "Identification of a novel chloride channel expressed in the endoplasmic
RT reticulum, Golgi apparatus, and nucleus.";
RL J. Biol. Chem. 276:20413-20418(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=Wistar; TISSUE=Brain;
RA Fukuhara C., Inouye S.T., Aoki K.;
RT "Isolation of a set of genes which was expressed specifically in the rat
RT suprachiasmatic nucleus.";
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Seems to act as a chloride ion channel (PubMed:11279057).
CC Plays a role in retina development (By similarity).
CC {ECO:0000250|UniProtKB:Q99LI2, ECO:0000269|PubMed:11279057}.
CC -!- SUBUNIT: Interacts with mitochondrial protein PIGBOS1 (via C-terminus);
CC the interaction occurs at the mitochondria-associated endoplasmic
CC reticulum (ER) membrane, a zone of contact between the ER and
CC mitochondrial membranes, but does not appear to play a role in ER-
CC mitochondria tethering and is not affected by ER stress (By
CC similarity). Interacts with CALR (By similarity).
CC {ECO:0000250|UniProtKB:Q96S66}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:11279057}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000269|PubMed:11279057};
CC Multi-pass membrane protein {ECO:0000255}. Nucleus membrane
CC {ECO:0000269|PubMed:11279057}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Within the endoplasmic reticulum (ER), localizes to
CC the mitochondria-associated ER membrane, a zone of contact between the
CC ER and mitochondrial membranes. {ECO:0000250|UniProtKB:Q96S66}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1; Synonyms=rMCLC-1;
CC IsoId=Q9WU61-1; Sequence=Displayed;
CC Name=2; Synonyms=rMCLC-2;
CC IsoId=Q9WU61-2; Sequence=VSP_027350;
CC Name=3; Synonyms=rMCLC-3;
CC IsoId=Q9WU61-3; Sequence=VSP_027352, VSP_027353;
CC Name=4; Synonyms=rMCLC-4;
CC IsoId=Q9WU61-4; Sequence=VSP_027350, VSP_027352, VSP_027353;
CC Name=5; Synonyms=rMCLC-5;
CC IsoId=Q9WU61-5; Sequence=VSP_027351, VSP_027354;
CC -!- TISSUE SPECIFICITY: Expressed in testis (spermatocytes), liver and lung
CC (at protein level). Expressed in spleen, liver, testis, kidney, heart,
CC brain and lung. {ECO:0000269|PubMed:11279057}.
CC -!- SIMILARITY: Belongs to the chloride channel MCLC family. {ECO:0000305}.
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DR EMBL; AB052919; BAB79264.1; -; mRNA.
DR EMBL; AB052920; BAB79265.1; -; mRNA.
DR EMBL; AB052921; BAB79266.1; -; mRNA.
DR EMBL; AB052922; BAB59019.1; -; mRNA.
DR EMBL; AB052923; BAB79267.1; -; mRNA.
DR EMBL; AF117330; AAD26207.1; -; mRNA.
DR EMBL; BC081736; AAH81736.1; -; mRNA.
DR RefSeq; NP_596905.1; NM_133414.1. [Q9WU61-1]
DR RefSeq; XP_006233189.1; XM_006233127.2.
DR RefSeq; XP_006233190.1; XM_006233128.3.
DR AlphaFoldDB; Q9WU61; -.
DR STRING; 10116.ENSRNOP00000042185; -.
DR iPTMnet; Q9WU61; -.
DR PhosphoSitePlus; Q9WU61; -.
DR jPOST; Q9WU61; -.
DR PaxDb; Q9WU61; -.
DR PeptideAtlas; Q9WU61; -.
DR PRIDE; Q9WU61; -.
DR GeneID; 170927; -.
DR KEGG; rno:170927; -.
DR UCSC; RGD:708359; rat. [Q9WU61-1]
DR CTD; 23155; -.
DR RGD; 708359; Clcc1.
DR eggNOG; ENOG502QSP7; Eukaryota.
DR InParanoid; Q9WU61; -.
DR OrthoDB; 1001950at2759; -.
DR PhylomeDB; Q9WU61; -.
DR PRO; PR:Q9WU61; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IDA:HGNC-UCL.
DR GO; GO:0005783; C:endoplasmic reticulum; IMP:HGNC-UCL.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IMP:HGNC-UCL.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044233; C:mitochondria-associated endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:HGNC-UCL.
DR GO; GO:0005254; F:chloride channel activity; IDA:RGD.
DR GO; GO:0006821; P:chloride transport; IDA:HGNC-UCL.
DR InterPro; IPR009231; Chloride_chnl_CLIC-like.
DR PANTHER; PTHR34093; PTHR34093; 1.
DR Pfam; PF05934; MCLC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloride; Chloride channel; Endoplasmic reticulum;
KW Golgi apparatus; Ion channel; Ion transport; Membrane; Nucleus;
KW Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..541
FT /note="Chloride channel CLIC-like protein 1"
FT /id="PRO_0000297684"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 362..381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 434
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99LI2"
FT MOD_RES 438
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96S66"
FT MOD_RES 482
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q96S66"
FT MOD_RES 504
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96S66"
FT VAR_SEQ 44..77
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:11279057"
FT /id="VSP_027350"
FT VAR_SEQ 394..395
FT /note="GD -> EP (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:11279057"
FT /id="VSP_027351"
FT VAR_SEQ 394
FT /note="G -> A (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:11279057"
FT /id="VSP_027352"
FT VAR_SEQ 395..541
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:11279057"
FT /id="VSP_027353"
FT VAR_SEQ 396..541
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:11279057"
FT /id="VSP_027354"
FT CONFLICT 57
FT /note="G -> D (in Ref. 3; AAH81736)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="A -> S (in Ref. 3; AAH81736)"
FT /evidence="ECO:0000305"
FT CONFLICT 417
FT /note="V -> A (in Ref. 3; AAH81736)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 541 AA; 61174 MW; 07954FC608C8FF40 CRC64;
MLCSLLLCGC LLLITGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGTSE KKEVNPGLSD
AQELSDCLQR LDSLTHKVDD CEKKKMKDYE SQSNPVFRRY LNKILIEAGK LGLPDEDRVD
VRYDAEILLT RQTLLEIQKF LSGEEWKPGA LDDALSDILT NFKSHDAEAW KWQFEDYFGV
DPYNVFMVLL CLLCIVALVA TELWTYVRWH TQLKRVCIIS FLVSLGWNWI YLYKVAFAQH
QANVAKMAPL NDVCAKKMDW TENLWEWFRI SWTYKDDPCQ KYYELLIVNP IWLVPPTKAL
AVTFTNFVTE PLKYIGKGTG EFIKALMKEI PVLLQIPVLV ILALAVLGFC YGAGQSVPML
RHFRGPEREP PRALEPDDRR RQKELDYRFH GGAGDADFSY RGPAGSIEQG PYDKMHVCER
DVLRQRQVNM RFPSGNKSPE VLRAFDLPDT EAQEHPEVVP SHKPSIVNTS LKETSELPRE
STLAECSQCA KDGSGQVPST AESSPIVEKA QLKTDSECRP HSTEAAAAAA RGTDPVSSPC
G
