CLCD_PSEPU
ID CLCD_PSEPU Reviewed; 236 AA.
AC P0A114; P11453;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=Carboxymethylenebutenolidase;
DE EC=3.1.1.45;
DE AltName: Full=Dienelactone hydrolase;
DE Short=DLH;
GN Name=clcD;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OG Plasmid pAC27.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AC859;
RX PubMed=3299368; DOI=10.1073/pnas.84.13.4460;
RA Frantz B., Chakrabarty A.M.;
RT "Organization and nucleotide sequence determination of a gene cluster
RT involved in 3-chlorocatechol degradation.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:4460-4464(1987).
CC -!- FUNCTION: Ring cleavage of cyclic ester dienelactone to produce
CC maleylacetate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC 2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC -!- SUBUNIT: Monomer.
CC -!- MISCELLANEOUS: Carboxymethylenebutenolidase is specific for
CC dienelactone and has no activity toward enol-lactones.
CC -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC {ECO:0000305}.
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DR EMBL; M16964; AAA98284.1; -; Genomic_DNA.
DR PIR; C27058; C27058.
DR PDB; 1GGV; X-ray; 2.50 A; A=1-232.
DR PDB; 1ZI6; X-ray; 1.70 A; A=1-236.
DR PDB; 1ZI8; X-ray; 1.40 A; A=1-236.
DR PDB; 1ZI9; X-ray; 1.50 A; A=1-236.
DR PDB; 1ZIC; X-ray; 1.70 A; A=1-236.
DR PDB; 1ZIX; X-ray; 1.80 A; A=1-236.
DR PDB; 1ZIY; X-ray; 1.90 A; A=1-236.
DR PDB; 1ZJ4; X-ray; 1.70 A; A=1-236.
DR PDB; 1ZJ5; X-ray; 1.70 A; A=1-236.
DR PDB; 4P92; X-ray; 1.65 A; A=1-236.
DR PDBsum; 1GGV; -.
DR PDBsum; 1ZI6; -.
DR PDBsum; 1ZI8; -.
DR PDBsum; 1ZI9; -.
DR PDBsum; 1ZIC; -.
DR PDBsum; 1ZIX; -.
DR PDBsum; 1ZIY; -.
DR PDBsum; 1ZJ4; -.
DR PDBsum; 1ZJ5; -.
DR PDBsum; 4P92; -.
DR AlphaFoldDB; P0A114; -.
DR SMR; P0A114; -.
DR ESTHER; psepu-clcd1; Dienelactone_hydrolase.
DR UniPathway; UPA00083; -.
DR EvolutionaryTrace; P0A114; -.
DR GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002925; Dienelactn_hydro.
DR Pfam; PF01738; DLH; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aromatic hydrocarbons catabolism; Hydrolase; Plasmid;
KW Serine esterase.
FT CHAIN 1..236
FT /note="Carboxymethylenebutenolidase"
FT /id="PRO_0000161570"
FT ACT_SITE 123
FT ACT_SITE 171
FT ACT_SITE 202
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 27..34
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 42..53
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 63..66
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 78..90
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 93..107
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 113..122
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 124..134
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 138..145
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 149..151
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 153..158
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 163..168
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 172..174
FT /evidence="ECO:0007829|PDB:1ZJ4"
FT HELIX 176..186
FT /evidence="ECO:0007829|PDB:1ZI8"
FT STRAND 192..197
FT /evidence="ECO:0007829|PDB:1ZI8"
FT TURN 202..205
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 214..228
FT /evidence="ECO:0007829|PDB:1ZI8"
FT HELIX 229..231
FT /evidence="ECO:0007829|PDB:1ZI8"
SQ SEQUENCE 236 AA; 25555 MW; F630F3D2793730D4 CRC64;
MLTEGISIQS YDGHTFGALV GSPAKAPAPV IVIAQEIFGV NAFMRETVSW LVDQGYAAVC
PDLYARQAPG TALDPQDERQ REQAYKLWQA FDMEAGVGDL EAAIRYARHQ PYSNGKVGLV
GYCLGGALAF LVAAKGYVDR AVGYYGVGLE KQLKKVPEVK HPALFHMGGQ DHFVPAPSRQ
LITEGFGANP LLQVHWYEEA GHSFARTSSS GYVASAAALA NERRLDFLAP LQSKKP
