CLCE_ARATH
ID CLCE_ARATH Reviewed; 710 AA.
AC Q8GX93; O65486; Q93XN4; Q9SVX1;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 02-FEB-2004, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Chloride channel protein CLC-e;
DE Short=AtCLC-e;
DE AltName: Full=CBS domain-containing protein CBSCLC3;
GN Name=CLC-E; Synonyms=CBSCLC3; OrderedLocusNames=At4g35440;
GN ORFNames=F15J1.10, F23E12.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Aerial part;
RA Vinauger-Douard M., Charon C., Lapous D., Allot M., Granier F., Bouchez D.,
RA Barbier-Brygoo H., Ephritikhine G.;
RT "Molecular and functional characterization of AtCLC-e, a new putative
RT Arabidopsis anion channel.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19400948; DOI=10.1186/1471-2164-10-200;
RA Kushwaha H.R., Singh A.K., Sopory S.K., Singla-Pareek S.L., Pareek A.;
RT "Genome wide expression analysis of CBS domain containing proteins in
RT Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their
RT developmental and stress regulation.";
RL BMC Genomics 10:200-200(2009).
CC -!- FUNCTION: Voltage-gated chloride channel.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GX93-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC42971.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA18726.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB54872.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80260.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF366367; AAK53390.1; -; mRNA.
DR EMBL; AL022604; CAA18726.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL117188; CAB54872.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161587; CAB80260.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86510.1; -; Genomic_DNA.
DR EMBL; AK118357; BAC42971.1; ALT_FRAME; mRNA.
DR PIR; T17122; T17122.
DR RefSeq; NP_567985.1; NM_119709.1. [Q8GX93-1]
DR AlphaFoldDB; Q8GX93; -.
DR SMR; Q8GX93; -.
DR STRING; 3702.AT4G35440.2; -.
DR TCDB; 2.A.49.6.3; the chloride carrier/channel (clc) family.
DR PaxDb; Q8GX93; -.
DR ProteomicsDB; 240976; -. [Q8GX93-1]
DR EnsemblPlants; AT4G35440.1; AT4G35440.1; AT4G35440. [Q8GX93-1]
DR GeneID; 829696; -.
DR Gramene; AT4G35440.1; AT4G35440.1; AT4G35440. [Q8GX93-1]
DR KEGG; ath:AT4G35440; -.
DR Araport; AT4G35440; -.
DR eggNOG; KOG0475; Eukaryota.
DR HOGENOM; CLU_015263_3_0_1; -.
DR InParanoid; Q8GX93; -.
DR OMA; ECINIAC; -.
DR PhylomeDB; Q8GX93; -.
DR PRO; PR:Q8GX93; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GX93; baseline and differential.
DR Genevisible; Q8GX93; AT.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IBA:GO_Central.
DR GO; GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR014743; Cl-channel_core.
DR InterPro; IPR001807; Cl-channel_volt-gated.
DR Pfam; PF00571; CBS; 1.
DR Pfam; PF00654; Voltage_CLC; 1.
DR PRINTS; PR00762; CLCHANNEL.
DR SMART; SM00116; CBS; 2.
DR SUPFAM; SSF54631; SSF54631; 1.
DR SUPFAM; SSF81340; SSF81340; 1.
DR PROSITE; PS51371; CBS; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; CBS domain; Chloride; Chloride channel; Ion channel;
KW Ion transport; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..710
FT /note="Chloride channel protein CLC-e"
FT /id="PRO_0000094469"
FT TRANSMEM 74..94
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TRANSMEM 667..687
FT /note="Helical; Name=13"
FT /evidence="ECO:0000255"
FT DOMAIN 565..624
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 640..702
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 500..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 503..518
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 519..534
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 297
FT /note="Missing (in Ref. 1; AAK53390)"
FT /evidence="ECO:0000305"
FT CONFLICT 453
FT /note="Missing (in Ref. 1; AAK53390)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 710 AA; 75556 MW; 2C7B325F7BF112DE CRC64;
MAATLPLCAA LRSPVSSRRF APIHKTDVPF QFNVVLSPFF GSVAIGGRIF PRLPAAKQET
DQDEVGFDQQ PSQELAIASA CLVGVLTGVS VVLFNNCVHL LRDFSWDGIP DRGASWLREA
PIGSNWLRVI LVPTIGGLVV SILNQLRESA GKSTGDSHSS LDRVKAVLRP FLKTVAACVT
LGTGNSLGPE GPSVEIGASI AKGVNSLFNK SPQTGFSLLA AGSAAGISSG FNAAVAGCFF
AVESVLWPSS STDSSTSLPN TTSMVILSAV TASVVSEIGL GSEPAFKVPD YDFRSPGELP
LYLLLGALCG LVSLALSRCT SSMTSAVDSL NKDAGIPKAV FPVMGGLSVG IIALVYPEVL
YWGFQNVDIL LEKRPFVKGL SADLLLQLVA VKIAATAWCR ASGLVGGYYA PSLFIGGAAG
MAYGKFIGLA LAQNPDFNLS ILEVASPQAY GLVGMAATLA GVCQVPLTAV LLLFELTQDY
RIVLPLLGAV GMSSWITSGQ SKRQETRETK ETRKRKSQEA VQSLTSSDDE SSTNNLCEVE
SSLCLDDSLN QSEELPKSIF VSEAMRTRFA TVMMSTSLEE ALTRMLIEKQ SCALIVDPDN
IFLGILTLSD IQEFSKARKE GNNRPKDIFV NDICSRSGGK CKVPWTVTPD MDLLAAQTIM
NKHELSHVAV VSGSIDAPRI HPVGVLDREC ITLTRRALAT RMYLLNSLYL
