ACHG_MOUSE
ID ACHG_MOUSE Reviewed; 519 AA.
AC P04760;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Acetylcholine receptor subunit gamma;
DE Flags: Precursor;
GN Name=Chrng; Synonyms=Acrg;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3010242; DOI=10.1093/nar/14.8.3539;
RA Yu L., Lapolla R.J., Davidson N.;
RT "Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence
RT and gene expression.";
RL Nucleic Acids Res. 14:3539-3555(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3755765; DOI=10.1002/jnr.490160106;
RA Boulter J., Evans K., Martin G., Mason P., Stengelin S., Goldman D.J.,
RA Heinemann S.F., Patrick J.;
RT "Isolation and sequence of cDNA clones coding for the precursor to the
RT gamma subunit of mouse muscle nicotinic acetylcholine receptor.";
RL J. Neurosci. Res. 16:37-49(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE OF 1-57.
RC TISSUE=Muscle;
RX PubMed=3480767; DOI=10.1016/0169-328x(87)90046-5;
RA Gardner P.D., Heinemann S.F., Patrick J.;
RT "Transcriptional regulation of nicotinic acetylcholine receptor genes:
RT identification of control elements of a gamma-subunit gene.";
RL Brain Res. 427:69-76(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
RC STRAIN=BALB/cJ;
RX PubMed=3244354; DOI=10.1128/mcb.8.12.5257-5267.1988;
RA Crowder C.M., Merlie J.P.;
RT "Stepwise activation of the mouse acetylcholine receptor delta- and gamma-
RT subunit genes in clonal cell lines.";
RL Mol. Cell. Biol. 8:5257-5267(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 115-170 (ISOFORMS LONG AND SHORT).
RX PubMed=7708706; DOI=10.1073/pnas.92.7.2686;
RA Mileo A.M., Monaco L., Palma E., Grassi F., Miledi R., Eusebi F.;
RT "Two forms of acetylcholine receptor gamma subunit in mouse muscle.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:2686-2690(1995).
CC -!- FUNCTION: After binding acetylcholine, the AChR responds by an
CC extensive change in conformation that affects all subunits and leads to
CC opening of an ion-conducting channel across the plasma membrane.
CC -!- SUBUNIT: Pentamer of two alpha chains, and one each of the beta, delta,
CC and gamma (in immature muscle) or epsilon (in mature muscle) chains.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P04760-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P04760-2; Sequence=VSP_000072;
CC -!- TISSUE SPECIFICITY: At least in myotubes of skeletal muscle.
CC -!- DEVELOPMENTAL STAGE: Both short and long isoforms are found in a 17
CC days old embryo, whereas the short isoform is the only isoform present
CC in the newborn muscle.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Acetylcholine receptor (TC 1.A.9.1) subfamily. Gamma/CHRNG sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; X03818; CAA27442.1; -; mRNA.
DR EMBL; X03819; CAA27443.1; -; mRNA.
DR EMBL; M30514; AAB63431.1; ALT_SEQ; mRNA.
DR EMBL; M27455; AAA70247.1; -; Genomic_DNA.
DR EMBL; M22381; AAA37152.1; -; Genomic_DNA.
DR EMBL; S77465; AAB33997.2; -; mRNA.
DR CCDS; CCDS35651.1; -. [P04760-1]
DR PIR; I56566; I56566.
DR AlphaFoldDB; P04760; -.
DR SMR; P04760; -.
DR ComplexPortal; CPX-252; Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-gamma.
DR STRING; 10090.ENSMUSP00000027470; -.
DR BindingDB; P04760; -.
DR ChEMBL; CHEMBL3038460; -.
DR GlyGen; P04760; 2 sites.
DR PhosphoSitePlus; P04760; -.
DR PaxDb; P04760; -.
DR PRIDE; P04760; -.
DR MGI; MGI:87895; Chrng.
DR eggNOG; KOG3645; Eukaryota.
DR InParanoid; P04760; -.
DR PhylomeDB; P04760; -.
DR Reactome; R-MMU-629587; Highly sodium permeable postsynaptic acetylcholine nicotinic receptors.
DR PRO; PR:P04760; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P04760; protein.
DR GO; GO:0005892; C:acetylcholine-gated channel complex; IDA:MGI.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0045211; C:postsynaptic membrane; IDA:MGI.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0022848; F:acetylcholine-gated cation-selective channel activity; IGI:MGI.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0015276; F:ligand-gated ion channel activity; ISO:MGI.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0095500; P:acetylcholine receptor signaling pathway; IC:ComplexPortal.
DR GO; GO:0006812; P:cation transport; ISO:MGI.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IGI:MGI.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0003009; P:skeletal muscle contraction; ISO:MGI.
DR Gene3D; 1.20.58.390; -; 2.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00254; NICOTINICR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..519
FT /note="Acetylcholine receptor subunit gamma"
FT /id="PRO_0000000335"
FT TOPO_DOM 23..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..476
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 150..164
FT /evidence="ECO:0000250"
FT VAR_SEQ 117..168
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:7708706"
FT /id="VSP_000072"
FT CONFLICT 231
FT /note="V -> G (in Ref. 2; AAB63431)"
FT /evidence="ECO:0000305"
FT CONFLICT 346
FT /note="L -> V (in Ref. 2; AAB63431)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 519 AA; 58745 MW; 3F435303564C8048 CRC64;
MQGGQRPHLL LLLLAVCLGA QSRNQEERLL ADLMRNYDPH LRPAERDSDV VNVSLKLTLT
NLISLNEREE ALTTNVWIEM QWCDYRLRWD PKDYEGLWIL RVPSTMVWRP DIVLENNVDG
VFEVALYCNV LVSPDGCIYW LPPAIFRSSC SISVTYFPFD WQNCSLIFQS QTYSTSEINL
QLSQEDGQAI EWIFIDPEAF TENGEWAIRH RPAKMLLDSV APAEEAGHQK VVFYLLIQRK
PLFYVINIIA PCVLISSVAI LIYFLPAKAG GQKCTVATNV LLAQTVFLFL VAKKVPETSQ
AVPLISKYLT FLMVVTILIV VNSVVVLNVS LRSPHTHSMA RGVRKLFLRL LPQLLRMHVR
PLAPAAVQDA RFRLQNGSSS GWPIMAREEG DLCLPRSELL FRQRQRNGLV QAVLEKLENG
PEVRQSQEFC GSLKQASPAI QACVDACNLM ARARRQQSHF DSGNEEWLLV GRVLDRVCFL
AMLSLFICGT AGIFLMAHYN QVPDLPFPGD PRPYLPLPD
