ID   CLCE_PSEAI              Reviewed;         352 AA.
AC   O87612;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Maleylacetate reductase;
DE            EC=1.3.1.32;
GN   Name=clcE;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JB2;
RX   PubMed=11571162; DOI=10.1128/aem.67.10.4603-4609.2001;
RA   Hickey W.J., Sabat G., Yuroff A.S., Arment A.R., Perez-Lesher J.;
RT   "Cloning, nucleotide sequencing, and functional analysis of a novel, mobile
RT   cluster of biodegradation genes from Pseudomonas aeruginosa strain JB2.";
RL   Appl. Environ. Microbiol. 67:4603-4609(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NAD(+) = H(+) + maleylacetate + NADH;
CC         Xref=Rhea:RHEA:16981, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.32;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NADP(+) = H(+) + maleylacetate + NADPH;
CC         Xref=Rhea:RHEA:16985, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.32;
CC   -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; AF087482; AAC69478.1; -; Genomic_DNA.
DR   AlphaFoldDB; O87612; -.
DR   SMR; O87612; -.
DR   BRENDA; 1.3.1.32; 5087.
DR   UniPathway; UPA00083; -.
DR   GO; GO:0018506; F:maleylacetate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08177; MAR; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR   InterPro; IPR034786; MAR.
DR   PANTHER; PTHR11496; PTHR11496; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..352
FT                   /note="Maleylacetate reductase"
FT                   /id="PRO_0000087845"
SQ   SEQUENCE   352 AA;  37755 MW;  D4CE1DD97D830E79 CRC64;
     MNFIHDYRSP RVIFGPDSLA RLPQELERLG IDRALVLTTP EQAPLGRQVA EPVIGHVAAF
     YDGATMHVPA LVAEEACKIA RTSEANGVIA IGGGSTIGLA KIVALRTELP IVAVPTTYAG
     SEMTSIFGIT EGGVKKTGRD ARVMPRAVIY EPRLTLELPL SISVTSAINA IAHAVEGLYA
     PDATPLLTIM AQEGIAATVR AISRMYQSPR DLQARGDALY GAWLCASVVG NVSMALHHKL
     CHTLGGTLDL PHAQTHTVVL PHALAYNARA VPDAMRVLRI ALGHDDPPTA LYELARDNGA
     PVALRDLGMR EEDIEHVGDL ALQDRYPNPR ELDRDALLAL LRDAYHGRPP SA