CLCF_ARATH
ID CLCF_ARATH Reviewed; 781 AA.
AC Q8RXR2; Q9ARM3; Q9LG04;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 02-FEB-2004, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Chloride channel protein CLC-f;
DE Short=AtCLC-f;
DE AltName: Full=CBS domain-containing protein CBSCLC1;
GN Name=CLC-F; Synonyms=CBSCLC1; OrderedLocusNames=At1g55620;
GN ORFNames=F20N2.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11283350; DOI=10.2307/3871354;
RA Rossberg M., Theres K., Acarkan A., Herrero R., Schmitt T., Schumacher K.,
RA Schmitz G., Schmidt R.;
RT "Comparative sequence analysis reveals extensive microcolinearity in the
RT lateral suppressor regions of the tomato, Arabidopsis, and Capsella
RT genomes.";
RL Plant Cell 13:979-988(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Aerial part;
RA Vinauger-Douard M., Charon C., Lapous D., Allot M., Granier F., Bouchez D.,
RA Barbier-Brygoo H., Ephritikhine G.;
RT "Molecular and functional characterization of AtCLC-f, a putative new
RT Arabidopsis chloride channel.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19400948; DOI=10.1186/1471-2164-10-200;
RA Kushwaha H.R., Singh A.K., Sopory S.K., Singla-Pareek S.L., Pareek A.;
RT "Genome wide expression analysis of CBS domain containing proteins in
RT Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their
RT developmental and stress regulation.";
RL BMC Genomics 10:200-200(2009).
CC -!- FUNCTION: Voltage-gated chloride channel.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8RXR2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RXR2-2; Sequence=VSP_009325;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79509.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ303348; CAC36386.1; -; mRNA.
DR EMBL; AF366368; AAK53391.1; -; mRNA.
DR EMBL; AC002328; AAF79509.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33274.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33275.1; -; Genomic_DNA.
DR EMBL; AY080722; AAL86324.1; -; mRNA.
DR RefSeq; NP_564698.1; NM_104438.4. [Q8RXR2-1]
DR RefSeq; NP_849813.1; NM_179482.3. [Q8RXR2-2]
DR AlphaFoldDB; Q8RXR2; -.
DR SMR; Q8RXR2; -.
DR STRING; 3702.AT1G55620.2; -.
DR PaxDb; Q8RXR2; -.
DR PRIDE; Q8RXR2; -.
DR ProteomicsDB; 246604; -. [Q8RXR2-1]
DR EnsemblPlants; AT1G55620.1; AT1G55620.1; AT1G55620. [Q8RXR2-2]
DR EnsemblPlants; AT1G55620.2; AT1G55620.2; AT1G55620. [Q8RXR2-1]
DR GeneID; 842011; -.
DR Gramene; AT1G55620.1; AT1G55620.1; AT1G55620. [Q8RXR2-2]
DR Gramene; AT1G55620.2; AT1G55620.2; AT1G55620. [Q8RXR2-1]
DR KEGG; ath:AT1G55620; -.
DR Araport; AT1G55620; -.
DR TAIR; locus:2020542; AT1G55620.
DR eggNOG; KOG0475; Eukaryota.
DR InParanoid; Q8RXR2; -.
DR PhylomeDB; Q8RXR2; -.
DR PRO; PR:Q8RXR2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RXR2; baseline and differential.
DR Genevisible; Q8RXR2; AT.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR014743; Cl-channel_core.
DR InterPro; IPR001807; Cl-channel_volt-gated.
DR Pfam; PF00571; CBS; 2.
DR Pfam; PF00654; Voltage_CLC; 1.
DR PRINTS; PR00762; CLCHANNEL.
DR SMART; SM00116; CBS; 2.
DR SUPFAM; SSF54631; SSF54631; 1.
DR SUPFAM; SSF81340; SSF81340; 1.
DR PROSITE; PS51371; CBS; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; CBS domain; Chloride; Chloride channel; Ion channel;
KW Ion transport; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..781
FT /note="Chloride channel protein CLC-f"
FT /id="PRO_0000094470"
FT TRANSMEM 129..149
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 502..522
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..543
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TRANSMEM 726..746
FT /note="Helical; Name=13"
FT /evidence="ECO:0000255"
FT DOMAIN 621..677
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 699..763
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 553..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..577
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..196
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_009325"
FT CONFLICT 643
FT /note="E -> K (in Ref. 5; AAL86324)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 781 AA; 83549 MW; BE9DEB3603D9E0D8 CRC64;
MSSGGAGEYN EDRHLLRSTD GDEVGIGGGE GDLDVESQSP AIRSGAGGVR DLFKHIDRRF
SLSGRRLSFK RMENIRVDRE RHNPSSSSAF SAAGEEDGGG ISNLHSVDDR NDEYGFDEEV
LGDSAPPEWA LLLIGCLIGV AAGICVAGFN KGVHVIHEWA WAGTPNEGAA WLRLQRLADT
WHRILLIPVT GGVIVGMMHG LLEILDQIRQ SNSSQRQGLD FLAGIYPVIK AIQAAVTLGT
GCSLGPEGPS VDIGKSCANG FALMMENNRE RRIALTAAGA ASGIASGFNA AVAGCFFAIE
TVLRPLRAEN SPPFTTAMII LASVISSTVS NALLGTQSAF TVPSYDLKSA AELPLYLILG
MLCGAVSVVF SRLVTWFTKS FDFIKDKFGL PAIVCPALGG LGAGIIALKY PGILYWGFTN
VEEILHTGKS ASAPGIWLLA QLAAAKVVAT ALCKGSGLVG GLYAPSLMIG AAVGAVFGGS
AAEIINRAIP GNAAVAQPQA YALVGMAATL ASMCSVPLTS VLLLFELTKD YRILLPLMGA
VGLAIWVPSV ANQGKESDSS EGRSTGRGYS SLSPSERKTE GVWRHTDNAD SLELTVIENP
DHNSFLDEET ILEDLKVMRV MSKNYVKVSS GTTLREARNI LKESHQNCIM VVDDDDFLAG
ILTHGDIRRY LSNNASTILD ENTCPVSSVC TKKISYRGQE RGLLTCYPDA TVGVAKELME
ARGVKQLPVV KRGEVIHKGK RRKLLGLLHY DSIWTFLRDE MSRRRSINDR RKDKEVGTNG
H
