位置:首页 > 蛋白库 > CLCN4_RAT
CLCN4_RAT
ID   CLCN4_RAT               Reviewed;         747 AA.
AC   P51794;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=H(+)/Cl(-) exchange transporter 4;
DE   AltName: Full=Chloride channel protein 4;
DE            Short=ClC-4;
DE   AltName: Full=Chloride transporter ClC-4;
GN   Name=Clcn4; Synonyms=Clcn4-2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=7730971; DOI=10.1113/jphysiol.1995.sp020560;
RA   Jentsch T.J., Guenther W., Pusch M., Schwappach B.;
RT   "Properties of voltage-gated chloride channels of the ClC gene family.";
RL   J. Physiol. (Lond.) 482:19-25(1995).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16222710; DOI=10.1002/jcp.20516;
RA   Suzuki T., Rai T., Hayama A., Sohara E., Suda S., Itoh T., Sasaki S.,
RA   Uchida S.;
RT   "Intracellular localization of ClC chloride channels and their ability to
RT   form hetero-oligomers.";
RL   J. Cell. Physiol. 206:792-798(2006).
CC   -!- FUNCTION: Strongly outwardly rectifying, electrogenic
CC       H(+)/Cl(-)exchanger which mediates the exchange of chloride ions
CC       against protons (By similarity). The CLC channel family contains both
CC       chloride channels and proton-coupled anion transporters that exchange
CC       chloride or another anion for protons (By similarity). The presence of
CC       conserved gating glutamate residues is typical for family members that
CC       function as antiporters (By similarity).
CC       {ECO:0000250|UniProtKB:P51793}.
CC   -!- SUBCELLULAR LOCATION: Early endosome membrane
CC       {ECO:0000269|PubMed:16222710}; Multi-pass membrane protein
CC       {ECO:0000255}. Late endosome membrane {ECO:0000269|PubMed:16222710};
CC       Multi-pass membrane protein {ECO:0000255}. Endoplasmic reticulum
CC       membrane {ECO:0000250|UniProtKB:P51793}; Multi-pass membrane protein
CC       {ECO:0000255}. Lysosome membrane {ECO:0000250|UniProtKB:P51793}; Multi-
CC       pass membrane protein {ECO:0000255}. Recycling endosome membrane
CC       {ECO:0000250|UniProtKB:P51793}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Localizes to late endosome membrane, lysosome
CC       membrane and recycling endosome membrane in the presence of CLCN3.
CC       {ECO:0000250|UniProtKB:P51793}.
CC   -!- TISSUE SPECIFICITY: Strongly expressed in liver and brain, but also in
CC       heart, muscle, kidney and spleen. {ECO:0000269|PubMed:7730971}.
CC   -!- SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family. ClC-
CC       4/CLCN4 subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z36944; CAA85406.1; -; mRNA.
DR   PIR; S47327; S47327.
DR   AlphaFoldDB; P51794; -.
DR   SMR; P51794; -.
DR   STRING; 10116.ENSRNOP00000056037; -.
DR   PaxDb; P51794; -.
DR   PeptideAtlas; P51794; -.
DR   PRIDE; P51794; -.
DR   RGD; 708381; Clcn4.
DR   eggNOG; KOG0475; Eukaryota.
DR   InParanoid; P51794; -.
DR   Reactome; R-RNO-2672351; Stimuli-sensing channels.
DR   PRO; PR:P51794; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0097546; C:ciliary base; ISO:RGD.
DR   GO; GO:0005769; C:early endosome; IBA:GO_Central.
DR   GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0010008; C:endosome membrane; ISS:UniProtKB.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR   GO; GO:0055037; C:recycling endosome; ISS:UniProtKB.
DR   GO; GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008021; C:synaptic vesicle; IBA:GO_Central.
DR   GO; GO:0015297; F:antiporter activity; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005254; F:chloride channel activity; ISO:RGD.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IBA:GO_Central.
DR   GO; GO:0005247; F:voltage-gated chloride channel activity; IBA:GO_Central.
DR   GO; GO:0006821; P:chloride transport; ISS:UniProtKB.
DR   GO; GO:1905515; P:non-motile cilium assembly; ISO:RGD.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   InterPro; IPR014743; Cl-channel_core.
DR   InterPro; IPR001807; Cl-channel_volt-gated.
DR   InterPro; IPR002246; Cl_channel-4.
DR   PANTHER; PTHR45711:SF2; PTHR45711:SF2; 1.
DR   Pfam; PF00571; CBS; 2.
DR   Pfam; PF00654; Voltage_CLC; 1.
DR   PRINTS; PR00762; CLCHANNEL.
DR   PRINTS; PR01115; CLCHANNEL4.
DR   SMART; SM00116; CBS; 2.
DR   SUPFAM; SSF54631; SSF54631; 1.
DR   SUPFAM; SSF81340; SSF81340; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   2: Evidence at transcript level;
KW   Antiport; ATP-binding; CBS domain; Chloride; Endoplasmic reticulum;
KW   Endosome; Ion transport; Lysosome; Membrane; Nucleotide-binding;
KW   Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..747
FT                   /note="H(+)/Cl(-) exchange transporter 4"
FT                   /id="PRO_0000094445"
FT   TOPO_DOM        1..54
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        55..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        138..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   INTRAMEM        170..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        187..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        211..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   INTRAMEM        242..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   INTRAMEM        258..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        278..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        320..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        429..449
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        454..473
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   INTRAMEM        501..515
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   INTRAMEM        519..530
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   INTRAMEM        531..534
FT                   /note="Note=Loop between two helices"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TRANSMEM        535..553
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   TOPO_DOM        554..747
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P35523"
FT   DOMAIN          587..653
FT                   /note="CBS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          684..742
FT                   /note="CBS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   REGION          1..50
FT                   /note="Required for localization in the endoplasmic
FT                   reticulum"
FT                   /evidence="ECO:0000250|UniProtKB:P51793"
FT   REGION          654..683
FT                   /note="Required for localization in the endoplasmic
FT                   reticulum"
FT                   /evidence="ECO:0000250|UniProtKB:P51793"
FT   MOTIF           167..171
FT                   /note="Selectivity filter part_1"
FT                   /evidence="ECO:0000250"
FT   MOTIF           209..213
FT                   /note="Selectivity filter part_2"
FT                   /evidence="ECO:0000250"
FT   MOTIF           454..458
FT                   /note="Selectivity filter part_3"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="chloride"
FT                   /ligand_id="ChEBI:CHEBI:17996"
FT                   /evidence="ECO:0000250"
FT   BINDING         456
FT                   /ligand="chloride"
FT                   /ligand_id="ChEBI:CHEBI:17996"
FT                   /evidence="ECO:0000250"
FT   BINDING         559
FT                   /ligand="chloride"
FT                   /ligand_id="ChEBI:CHEBI:17996"
FT                   /evidence="ECO:0000250"
FT   BINDING         597
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         618..620
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         725..728
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   SITE            211
FT                   /note="Mediates proton transfer from the outer aqueous
FT                   phase to the interior of the protein; involved in linking
FT                   H(+) and Cl(-) transport"
FT                   /evidence="ECO:0000250"
FT   SITE            268
FT                   /note="Mediates proton transfer from the protein to the
FT                   inner aqueous phase"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   747 AA;  83701 MW;  E86B5732248BA0C3 CRC64;
     MDFLDEPFPD VGTYEDFHTI DWLREKSRDT DRHRKITSKS KESIWEFIKS LLDAWSGWVV
     MLLIGLLAGT LAGVIDLAVD WMTDLKEGVC LSAFWYSHEQ CCWTSNETTF EDRDKCPLWQ
     KWSELLLSQS EGASAYILNY LMYILWALLF AFLAVSLVRV FAPYACGSGI PEIKTILSGF
     IIRGYLGKWT LLIKTVTLVL VVSSGLSLGK EGPLVHVACC CGNFFSSLFS KYSKNEGKRR
     EVLSAAAAAG VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN
     SRLVLFYVEY HTPWYMAELF PFILLGVFGG LWGTVFTRCN IAWCRRRKTT RLGKYPVLEV
     IVVTAITAII AYPNPYTRQS TSELISELFN DCGALESSQL CDYINDPNMT RPVDDIPDRP
     AGVGVYTAMW QLALALIFKI VITIFTFGMK IPSGLFIPSM AVGAMAGRMV GIGVEQLAYH
     HHDWIIFRNW CRPGADCVTP GLYAMVGAAA CLGGVTRMTV SLVVIMFELT GGLEYIVPLM
     AAAVTSKWVA DAFGKEGIYE AHIHLNGYPF LDVKDEFTHR TLATDVMRPR RGEPPLSVLT
     QDSMTVEDVE TLIKETDYNG FPVVVSRDSE RLIGFAQRRE LILAIKNARQ RQEGIVSNSI
     MYFTEEPPEL PANSPHPLKL RRILNLSPFT VTDHTPMETV VDIFRKLGLR QCLVTRSGRL
     LGIITKKDVL RHMAQMANQD PESIIFN
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024