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CLCN5_RAT
ID   CLCN5_RAT               Reviewed;         816 AA.
AC   P51796; A0A0G2K839; P70642;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 2.
DT   03-AUG-2022, entry version 153.
DE   RecName: Full=H(+)/Cl(-) exchange transporter 5;
DE   AltName: Full=Chloride channel protein 5;
DE            Short=ClC-5;
DE   AltName: Full=Chloride transporter ClC-5;
GN   Name=Clcn5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=8537381; DOI=10.1074/jbc.270.52.31172;
RA   Steinmeyer K., Schwappach B., Bens M., Vandewalle A., Jentsch T.J.;
RT   "Cloning and functional expression of rat CLC-5, a chloride channel related
RT   to kidney disease.";
RL   J. Biol. Chem. 270:31172-31177(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC   TISSUE=Kidney;
RX   PubMed=8626585; DOI=10.1074/jbc.271.17.10210;
RA   Sakamoto H., Kawasaki M., Uchida S., Sasaki S., Marumo F.;
RT   "Identification of a new outwardly rectifying Cl- channel that belongs to a
RT   subfamily of the ClC Cl- channels.";
RL   J. Biol. Chem. 271:10210-10216(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [4]
RP   FUNCTION.
RX   PubMed=19261613; DOI=10.1074/jbc.m901170200;
RA   Bergsdorf E.-Y., Zdebik A.A., Jentsch T.J.;
RT   "Residues important for nitrate/proton coupling in plant and mammalian CLC
RT   transporters.";
RL   J. Biol. Chem. 284:11184-11193(2009).
CC   -!- FUNCTION: Proton-coupled chloride transporter. Functions as antiport
CC       system and exchanges chloride ions against protons. Important for
CC       normal acidification of the endosome lumen. May play an important role
CC       in renal tubular function (By similarity). The CLC channel family
CC       contains both chloride channels and proton-coupled anion transporters
CC       that exchange chloride or another anion for protons. The absence of
CC       conserved gating glutamate residues is typical for family members that
CC       function as channels (Probable). {ECO:0000250|UniProtKB:P51795,
CC       ECO:0000269|PubMed:19261613, ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 chloride(in) + H(+)(out) = 2 chloride(out) + H(+)(in);
CC         Xref=Rhea:RHEA:29567, ChEBI:CHEBI:15378, ChEBI:CHEBI:17996;
CC         Evidence={ECO:0000250|UniProtKB:P51795};
CC   -!- SUBUNIT: Interacts with NEDD4 and NEDD4L.
CC       {ECO:0000250|UniProtKB:P51795}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000250|UniProtKB:P51795}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P51795}. Endosome membrane
CC       {ECO:0000250|UniProtKB:P51795}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P51795}. Cell membrane
CC       {ECO:0000250|UniProtKB:P51795}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P51795}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P51796-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P51796-2; Sequence=VSP_060659;
CC   -!- TISSUE SPECIFICITY: Kidney specific.
CC   -!- PTM: Ubiquitinated by NEDD4L in the presence of albumin; which promotes
CC       endocytosis and proteasomal degradation.
CC       {ECO:0000250|UniProtKB:P51795}.
CC   -!- SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family. ClC-
CC       5/CLCN5 subfamily. {ECO:0000305}.
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DR   EMBL; Z56277; CAA91216.1; -; mRNA.
DR   EMBL; D50497; BAA09091.1; -; mRNA.
DR   EMBL; AABR07037207; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AABR07037208; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_058802.1; NM_017106.1. [P51796-2]
DR   AlphaFoldDB; P51796; -.
DR   SMR; P51796; -.
DR   STRING; 10116.ENSRNOP00000003895; -.
DR   PhosphoSitePlus; P51796; -.
DR   PaxDb; P51796; -.
DR   ABCD; P51796; 2 sequenced antibodies.
DR   Ensembl; ENSRNOT00000003895; ENSRNOP00000003895; ENSRNOG00000002862. [P51796-2]
DR   Ensembl; ENSRNOT00000079054; ENSRNOP00000074459; ENSRNOG00000002862. [P51796-1]
DR   GeneID; 25749; -.
DR   KEGG; rno:25749; -.
DR   UCSC; RGD:2362; rat. [P51796-1]
DR   CTD; 1184; -.
DR   RGD; 2362; Clcn5.
DR   eggNOG; KOG0475; Eukaryota.
DR   GeneTree; ENSGT00940000153763; -.
DR   HOGENOM; CLU_003181_2_1_1; -.
DR   InParanoid; P51796; -.
DR   OMA; CLDWTPW; -.
DR   OrthoDB; 271925at2759; -.
DR   PhylomeDB; P51796; -.
DR   Reactome; R-RNO-2672351; Stimuli-sensing channels.
DR   PRO; PR:P51796; -.
DR   Proteomes; UP000002494; Chromosome X.
DR   Bgee; ENSRNOG00000002862; Expressed in adult mammalian kidney and 19 other tissues.
DR   ExpressionAtlas; P51796; baseline and differential.
DR   Genevisible; P51796; RN.
DR   GO; GO:0045177; C:apical part of cell; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005769; C:early endosome; IBA:GO_Central.
DR   GO; GO:0005768; C:endosome; IDA:RGD.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR   GO; GO:0008021; C:synaptic vesicle; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IBA:GO_Central.
DR   GO; GO:0005247; F:voltage-gated chloride channel activity; IDA:RGD.
DR   GO; GO:0006821; P:chloride transport; IDA:RGD.
DR   GO; GO:0006897; P:endocytosis; ISO:RGD.
DR   GO; GO:0003014; P:renal system process; ISO:RGD.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   InterPro; IPR014743; Cl-channel_core.
DR   InterPro; IPR001807; Cl-channel_volt-gated.
DR   InterPro; IPR002247; Cl_channel-5.
DR   Pfam; PF00571; CBS; 2.
DR   Pfam; PF00654; Voltage_CLC; 1.
DR   PRINTS; PR00762; CLCHANNEL.
DR   PRINTS; PR01116; CLCHANNEL5.
DR   SMART; SM00116; CBS; 2.
DR   SUPFAM; SSF54631; SSF54631; 1.
DR   SUPFAM; SSF81340; SSF81340; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Antiport; ATP-binding; CBS domain; Cell membrane;
KW   Chloride; Endosome; Golgi apparatus; Ion transport; Membrane;
KW   Nucleotide-binding; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport; Ubl conjugation.
FT   CHAIN           1..816
FT                   /note="H(+)/Cl(-) exchange transporter 5"
FT                   /id="PRO_0000094448"
FT   TOPO_DOM        1..124
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        125..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        208..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        240..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        256..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        281..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        312..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        328..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        348..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        389..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        422..442
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        498..518
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        523..542
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        570..584
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        585..587
FT                   /note="Note=Loop between two helices"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        588..599
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   INTRAMEM        600..604
FT                   /note="Note=Loop between two helices"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        605..622
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        623..816
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   DOMAIN          656..720
FT                   /note="CBS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          752..811
FT                   /note="CBS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   MOTIF           237..241
FT                   /note="Selectivity filter part_1"
FT                   /evidence="ECO:0000250"
FT   MOTIF           279..283
FT                   /note="Selectivity filter part_2"
FT                   /evidence="ECO:0000250"
FT   MOTIF           523..527
FT                   /note="Selectivity filter part_3"
FT                   /evidence="ECO:0000250"
FT   BINDING         238
FT                   /ligand="chloride"
FT                   /ligand_id="ChEBI:CHEBI:17996"
FT                   /evidence="ECO:0000250"
FT   BINDING         525
FT                   /ligand="chloride"
FT                   /ligand_id="ChEBI:CHEBI:17996"
FT                   /evidence="ECO:0000250"
FT   BINDING         628
FT                   /ligand="chloride"
FT                   /ligand_id="ChEBI:CHEBI:17996"
FT                   /evidence="ECO:0000250"
FT   BINDING         666
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P51795"
FT   BINDING         687..689
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P51795"
FT   BINDING         794..797
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P51795"
FT   SITE            281
FT                   /note="Mediates proton transfer from the outer aqueous
FT                   phase to the interior of the protein; involved in linking
FT                   H(+) and Cl(-) transport"
FT                   /evidence="ECO:0000250"
FT   SITE            338
FT                   /note="Mediates proton transfer from the protein to the
FT                   inner aqueous phase"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..70
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_060659"
FT   CONFLICT        385
FT                   /note="H -> Y (in Ref. 2; BAA09091)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   816 AA;  90687 MW;  68AE3812E820141D CRC64;
     MAMWQGAMDN RGFHQGSFSS FQSSSSDEDL MDIPGTAMDF SMRDDVPPLD REIEGNKSYN
     GGGIGSSNRI MDFLEEPIPG VGTYDDFNTI DWVREKSRDR DRHREITNKS KESTWALIHS
     VSDAFSGWLL MLLIGLLSGS LAGLIDISAH WMTDLKEGIC TGGFWFNHEH CCWNSEHVTF
     EDRDKCPEWN SWSQLIISTD QGAFAYIVNY FMYVLWALLF AFLAVSLVKA FAPYACGSGI
     PEIKTILSGF IIRGYLGKWT LVIKTITLVL AVSSGLSLGK EGPLVHVACC CGNILCHCFN
     KYRKNEAKRR EVLSAAAAAG VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF
     TLRSINPFGN SRLVLFYVEF HTPWHLFELV PFIVLGIFGG LWGALFIRTN IAWCRKRKTT
     QLGKYPVVEV LIVTAITAIL AFPNEYTRMS TSELISELFN DCGLLDSSKL CDYENHFNTS
     KGGELPDRPA GVGVYSAMWQ LALTLILKIV ITIFTFGMKI PSGLFIPSMA VGAIAGRLLG
     VGMEQLAYYH HDWGIFNSWC SQGADCITPG LYAMVGAAAC LGGVTRMTVS LVVIMFELTG
     GLEYIVPLMA AAMTSKWVAD ALGREGIYDA HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR
     NDPLLTVLTQ DSMTVEDVET IISETTYSGF PVVVSRESQR LVGFVLRRDL IISIENARKK
     QDGVVSTSII YFTEHSPPMP PYTPPTLKLR NILDLSPFTV TDLTPMEIVV DIFRKLGLRQ
     CLVTHNGRLL GIITKKDVLK HIAQMANQDP ESILFN
 
 
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