CLD12_MOUSE
ID CLD12_MOUSE Reviewed; 244 AA.
AC Q9ET43; Q3TM65; Q8BH13;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2003, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Claudin-12;
GN Name=Cldn12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, Lung, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-237.
RC STRAIN=ICR;
RA Kiuchi Y., Morita K., Furuse M., Tsukita S.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a major role in tight junction-specific obliteration of
CC the intercellular space, through calcium-independent cell-adhesion
CC activity. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with OCLN. {ECO:0000250|UniProtKB:P56749}.
CC -!- SUBCELLULAR LOCATION: Cell junction, tight junction {ECO:0000250}. Cell
CC membrane {ECO:0000250|UniProtKB:P56749}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the claudin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK039672; BAC30414.1; -; mRNA.
DR EMBL; AK049331; BAC33687.1; -; mRNA.
DR EMBL; AK132155; BAE21001.1; -; mRNA.
DR EMBL; AK166110; BAE38577.1; -; mRNA.
DR EMBL; BC024057; AAH24057.1; -; mRNA.
DR EMBL; AF247664; AAF98801.1; -; mRNA.
DR CCDS; CCDS19074.1; -.
DR RefSeq; NP_001180588.1; NM_001193659.1.
DR RefSeq; NP_001180589.1; NM_001193660.1.
DR RefSeq; NP_001180590.1; NM_001193661.1.
DR RefSeq; NP_075028.1; NM_022890.2.
DR RefSeq; XP_006503661.1; XM_006503598.3.
DR RefSeq; XP_011238975.1; XM_011240673.2.
DR RefSeq; XP_011238976.1; XM_011240674.1.
DR AlphaFoldDB; Q9ET43; -.
DR IntAct; Q9ET43; 1.
DR MINT; Q9ET43; -.
DR STRING; 10090.ENSMUSP00000061928; -.
DR iPTMnet; Q9ET43; -.
DR PhosphoSitePlus; Q9ET43; -.
DR jPOST; Q9ET43; -.
DR MaxQB; Q9ET43; -.
DR PaxDb; Q9ET43; -.
DR PeptideAtlas; Q9ET43; -.
DR PRIDE; Q9ET43; -.
DR ProteomicsDB; 283290; -.
DR Antibodypedia; 35206; 222 antibodies from 27 providers.
DR DNASU; 64945; -.
DR Ensembl; ENSMUST00000060947; ENSMUSP00000061928; ENSMUSG00000046798.
DR Ensembl; ENSMUST00000115445; ENSMUSP00000111105; ENSMUSG00000046798.
DR Ensembl; ENSMUST00000115446; ENSMUSP00000111106; ENSMUSG00000046798.
DR Ensembl; ENSMUST00000179804; ENSMUSP00000136988; ENSMUSG00000046798.
DR GeneID; 64945; -.
DR KEGG; mmu:64945; -.
DR UCSC; uc008wip.2; mouse.
DR CTD; 9069; -.
DR MGI; MGI:1929288; Cldn12.
DR VEuPathDB; HostDB:ENSMUSG00000046798; -.
DR eggNOG; ENOG502QR4G; Eukaryota.
DR GeneTree; ENSGT00400000022250; -.
DR HOGENOM; CLU_063070_0_0_1; -.
DR InParanoid; Q9ET43; -.
DR OMA; FYNTHLN; -.
DR OrthoDB; 933771at2759; -.
DR PhylomeDB; Q9ET43; -.
DR TreeFam; TF331972; -.
DR BioGRID-ORCS; 64945; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q9ET43; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9ET43; protein.
DR Bgee; ENSMUSG00000046798; Expressed in dorsal pancreas and 213 other tissues.
DR Genevisible; Q9ET43; MM.
DR GO; GO:0005923; C:bicellular tight junction; ISS:UniProtKB.
DR GO; GO:0071944; C:cell periphery; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016328; C:lateral plasma membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0070160; C:tight junction; IDA:ARUK-UCL.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0016338; P:calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules; ISS:UniProtKB.
DR InterPro; IPR013287; Claudin12.
DR InterPro; IPR017974; Claudin_CS.
DR PANTHER; PTHR16703; PTHR16703; 1.
DR PRINTS; PR01872; CLAUDIN12.
DR PROSITE; PS01346; CLAUDIN; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Membrane; Phosphoprotein; Reference proteome;
KW Tight junction; Transmembrane; Transmembrane helix.
FT CHAIN 1..244
FT /note="Claudin-12"
FT /id="PRO_0000144766"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..87
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..174
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 228
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P56749"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 244 AA; 26995 MW; 5A49F3BD7C25C87E CRC64;
MGCRDVHAAT VLSFLCGIAS VAGLFAGTLL PNWRKLRLIT FNRNEKNLTI YTGLWVKCAR
YDGSSDCLMY DRTWYLSVDQ LDLRVLQFAL PLSIVIAMGA LLLCLIGMCN TAFNSSVPNI
KLAKCLVNSA GCHLVAGLLF FLAGTVSLSP SIWAIFYNSH LNRKFEPVFT FDYAVFVTIA
SSGGLFMTAL LLFVWYCACK SLSSPFWQPL YSHAPGMHTY SQPYSSRSRL SAIEIDIPVV
SHST
