CLD18_MOUSE
ID CLD18_MOUSE Reviewed; 264 AA.
AC P56857; Q91ZY9; Q91ZZ0; Q91ZZ1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Claudin-18;
GN Name=Cldn18;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP ALTERNATIVE SPLICING.
RX PubMed=11585919; DOI=10.1128/mcb.21.21.7380-7390.2001;
RA Niimi T., Nagashima K., Ward J.M., Minoo P., Zimonjic D.B., Popescu N.C.,
RA Kimura S.;
RT "Claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
RT homeodomain transcription factor, encodes lung- and stomach-specific
RT isoforms through alternative splicing.";
RL Mol. Cell. Biol. 21:7380-7390(2001).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a major role in tight junction-specific obliteration of
CC the intercellular space, through calcium-independent cell-adhesion
CC activity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell junction, tight junction
CC {ECO:0000269|PubMed:11585919}. Cell membrane
CC {ECO:0000269|PubMed:11585919}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes to tight junctions in epithelial cells.
CC {ECO:0000269|PubMed:11585919}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A1.1;
CC IsoId=P56857-1; Sequence=Displayed;
CC Name=A1.2;
CC IsoId=P56857-2; Sequence=VSP_001104, VSP_001105;
CC Name=A2.1;
CC IsoId=P56857-3; Sequence=VSP_001103;
CC Name=A2.2;
CC IsoId=P56857-4; Sequence=VSP_001103, VSP_001104, VSP_001105;
CC -!- TISSUE SPECIFICITY: Isoform A1.1: Expressed in lung (PubMed:11585919).
CC Isoform A1.2: Expressed in lung (PubMed:11585919). Isoform A2.1:
CC Expressed in stomach (PubMed:11585919). Isoform A2.2: Expressed in
CC stomach (PubMed:11585919).
CC -!- SIMILARITY: Belongs to the claudin family. {ECO:0000305}.
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DR EMBL; AF221068; AAF26447.1; -; mRNA.
DR EMBL; AF349450; AAL15635.1; -; mRNA.
DR EMBL; AF349451; AAL15636.1; -; mRNA.
DR EMBL; AF349453; AAL15638.1; -; mRNA.
DR CCDS; CCDS23437.1; -. [P56857-1]
DR CCDS; CCDS57692.1; -. [P56857-2]
DR CCDS; CCDS57693.1; -. [P56857-4]
DR CCDS; CCDS57694.1; -. [P56857-3]
DR RefSeq; NP_001181850.1; NM_001194921.1. [P56857-3]
DR RefSeq; NP_001181851.1; NM_001194922.1. [P56857-2]
DR RefSeq; NP_001181852.1; NM_001194923.1. [P56857-4]
DR RefSeq; NP_062789.1; NM_019815.3. [P56857-1]
DR AlphaFoldDB; P56857; -.
DR SMR; P56857; -.
DR DIP; DIP-48956N; -.
DR IntAct; P56857; 2.
DR STRING; 10090.ENSMUSP00000035048; -.
DR TCDB; 1.H.1.1.8; the claudin tight junction (claudin1) family.
DR iPTMnet; P56857; -.
DR PhosphoSitePlus; P56857; -.
DR MaxQB; P56857; -.
DR PaxDb; P56857; -.
DR PRIDE; P56857; -.
DR ProteomicsDB; 285482; -. [P56857-1]
DR ProteomicsDB; 285483; -. [P56857-2]
DR ProteomicsDB; 285484; -. [P56857-3]
DR ProteomicsDB; 285485; -. [P56857-4]
DR Antibodypedia; 4575; 209 antibodies from 29 providers.
DR DNASU; 56492; -.
DR Ensembl; ENSMUST00000035048; ENSMUSP00000035048; ENSMUSG00000032473. [P56857-1]
DR Ensembl; ENSMUST00000112882; ENSMUSP00000108503; ENSMUSG00000032473. [P56857-3]
DR Ensembl; ENSMUST00000131922; ENSMUSP00000117382; ENSMUSG00000032473. [P56857-4]
DR Ensembl; ENSMUST00000136429; ENSMUSP00000115782; ENSMUSG00000032473. [P56857-2]
DR GeneID; 56492; -.
DR KEGG; mmu:56492; -.
DR UCSC; uc009rep.2; mouse. [P56857-1]
DR UCSC; uc009req.2; mouse. [P56857-2]
DR UCSC; uc009rer.2; mouse. [P56857-4]
DR UCSC; uc009res.2; mouse. [P56857-3]
DR CTD; 51208; -.
DR MGI; MGI:1929209; Cldn18.
DR VEuPathDB; HostDB:ENSMUSG00000032473; -.
DR eggNOG; ENOG502QTRB; Eukaryota.
DR GeneTree; ENSGT00940000158655; -.
DR HOGENOM; CLU_076370_2_1_1; -.
DR InParanoid; P56857; -.
DR OMA; TICQVMG; -.
DR PhylomeDB; P56857; -.
DR TreeFam; TF331936; -.
DR BioGRID-ORCS; 56492; 1 hit in 73 CRISPR screens.
DR PRO; PR:P56857; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; P56857; protein.
DR Bgee; ENSMUSG00000032473; Expressed in mucous cell of stomach and 25 other tissues.
DR Genevisible; P56857; MM.
DR GO; GO:0005923; C:bicellular tight junction; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0070830; P:bicellular tight junction assembly; IBA:GO_Central.
DR GO; GO:0016338; P:calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules; ISS:UniProtKB.
DR GO; GO:0007155; P:cell adhesion; IBA:GO_Central.
DR GO; GO:0048565; P:digestive tract development; IMP:MGI.
DR GO; GO:0045779; P:negative regulation of bone resorption; IMP:MGI.
DR GO; GO:2001205; P:negative regulation of osteoclast development; IMP:MGI.
DR GO; GO:1900181; P:negative regulation of protein localization to nucleus; IDA:MGI.
DR GO; GO:0034504; P:protein localization to nucleus; IDA:MGI.
DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR GO; GO:0071847; P:TNFSF11-mediated signaling pathway; IMP:MGI.
DR InterPro; IPR006187; Claudin.
DR InterPro; IPR003928; Claudin18.
DR InterPro; IPR017974; Claudin_CS.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR PANTHER; PTHR12002; PTHR12002; 1.
DR PANTHER; PTHR12002:SF9; PTHR12002:SF9; 1.
DR Pfam; PF00822; PMP22_Claudin; 1.
DR PRINTS; PR01448; CLAUDIN18.
DR PROSITE; PS01346; CLAUDIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; Cell membrane; Membrane;
KW Phosphoprotein; Reference proteome; Tight junction; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..264
FT /note="Claudin-18"
FT /id="PRO_0000144780"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 241..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..264
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 217
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..69
FT /note="MATTTCQVVGLLLSLLGLAGCIAATGMDMWSTQDLYDNPVTAVFQYEGLWRS
FT CVQQSSGFTECRPYFTI -> MSVTACQGLGFVVSLIGFAGIIAATCMDQWSTQDLYNN
FT PVTAVFNYQGLWRSCVRESSGFTECRGYFTL (in isoform A2.1 and
FT isoform A2.2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001103"
FT VAR_SEQ 208
FT /note="N -> K (in isoform A1.2 and isoform A2.2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001104"
FT VAR_SEQ 209..264
FT /note="Missing (in isoform A1.2 and isoform A2.2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001105"
SQ SEQUENCE 264 AA; 28122 MW; 3CA0D441C4705653 CRC64;
MATTTCQVVG LLLSLLGLAG CIAATGMDMW STQDLYDNPV TAVFQYEGLW RSCVQQSSGF
TECRPYFTIL GLPAMLQAVR ALMIVGIVLG VIGILVSIFA LKCIRIGSMD DSAKAKMTLT
SGILFIISGI CAIIGVSVFA NMLVTNFWMS TANMYSGMGG MGGMVQTVQT RYTFGAALFV
GWVAGGLTLI GGVMMCIACR GLTPDDSNFK AVSYHASGQN VAYRPGGFKA STGFGSNTRN
KKIYDGGART EDDEQSHPTK YDYV
