CLD4_BOVIN
ID CLD4_BOVIN Reviewed; 209 AA.
AC Q6BBL6; Q2KJ40;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Claudin-4;
GN Name=CLDN4;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RA Ohta H., Takiguchi M., Inaba M.;
RT "Localization of claudin proteins in bovine kidneys.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Uterus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Channel-forming tight junction protein that mediates
CC paracellular chloride transport in the kidney. Plays a critical role in
CC the paracellular reabsorption of filtered chloride in the kidney
CC collecting ducts. Claudins play a major role in tight junction-specific
CC obliteration of the intercellular space, through calcium-independent
CC cell-adhesion activity. {ECO:0000250|UniProtKB:O35054}.
CC -!- SUBUNIT: Interacts with EPHA2; phosphorylates CLDN4 and may regulate
CC tight junctions (By similarity). Directly interacts with TJP1/ZO-1,
CC TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with CLDN1 (By
CC similarity). Interacts with CLDN8 (By similarity).
CC {ECO:0000250|UniProtKB:O14493, ECO:0000250|UniProtKB:O35054}.
CC -!- SUBCELLULAR LOCATION: Cell junction, tight junction
CC {ECO:0000250|UniProtKB:O35054}. Cell membrane
CC {ECO:0000250|UniProtKB:O35054}; Multi-pass membrane protein
CC {ECO:0000255}. Note=CLDN4 is required for tight junction localization
CC in the kidney. {ECO:0000250|UniProtKB:O35054}.
CC -!- PTM: Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1
CC and alters interaction with TJP1 (By similarity).
CC {ECO:0000250|UniProtKB:O14493}.
CC -!- SIMILARITY: Belongs to the claudin family. {ECO:0000305}.
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DR EMBL; AB185928; BAD34533.1; -; mRNA.
DR EMBL; BC105534; AAI05535.1; -; mRNA.
DR RefSeq; NP_001014413.1; NM_001014391.2.
DR AlphaFoldDB; Q6BBL6; -.
DR SMR; Q6BBL6; -.
DR STRING; 9913.ENSBTAP00000037110; -.
DR PaxDb; Q6BBL6; -.
DR PRIDE; Q6BBL6; -.
DR Ensembl; ENSBTAT00000037275; ENSBTAP00000037110; ENSBTAG00000026278.
DR GeneID; 414921; -.
DR KEGG; bta:414921; -.
DR CTD; 1364; -.
DR VEuPathDB; HostDB:ENSBTAG00000026278; -.
DR VGNC; VGNC:27416; CLDN4.
DR eggNOG; ENOG502QSCN; Eukaryota.
DR GeneTree; ENSGT00940000154762; -.
DR HOGENOM; CLU_076370_1_2_1; -.
DR InParanoid; Q6BBL6; -.
DR OMA; RTEKPYS; -.
DR OrthoDB; 1231389at2759; -.
DR TreeFam; TF331936; -.
DR Proteomes; UP000009136; Chromosome 25.
DR Bgee; ENSBTAG00000026278; Expressed in placenta and 80 other tissues.
DR GO; GO:0005923; C:bicellular tight junction; ISS:UniProtKB.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005254; F:chloride channel activity; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0070830; P:bicellular tight junction assembly; IBA:GO_Central.
DR GO; GO:0016338; P:calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules; ISS:UniProtKB.
DR GO; GO:0007155; P:cell adhesion; IBA:GO_Central.
DR GO; GO:0061436; P:establishment of skin barrier; IEA:Ensembl.
DR GO; GO:0030335; P:positive regulation of cell migration; IEA:Ensembl.
DR GO; GO:1905050; P:positive regulation of metallopeptidase activity; IEA:Ensembl.
DR GO; GO:0090303; P:positive regulation of wound healing; IEA:Ensembl.
DR GO; GO:0022604; P:regulation of cell morphogenesis; IEA:Ensembl.
DR GO; GO:0070293; P:renal absorption; ISS:UniProtKB.
DR InterPro; IPR006187; Claudin.
DR InterPro; IPR003550; Claudin4.
DR InterPro; IPR017974; Claudin_CS.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR PANTHER; PTHR12002; PTHR12002; 1.
DR Pfam; PF00822; PMP22_Claudin; 1.
DR PRINTS; PR01379; CLAUDIN4.
DR PROSITE; PS01346; CLAUDIN; 1.
PE 2: Evidence at transcript level;
KW Cell junction; Cell membrane; Chloride; Chloride channel; Disulfide bond;
KW Ion channel; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Tight junction; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..209
FT /note="Claudin-4"
FT /id="PRO_0000144741"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..117
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..160
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..209
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..103
FT /note="Interaction with EPHA2"
FT /evidence="ECO:0000250|UniProtKB:O14493"
FT REGION 208..209
FT /note="Interactions with TJP1, TJP2 and TJP3"
FT /evidence="ECO:0000250|UniProtKB:O35054"
FT MOD_RES 208
FT /note="Phosphotyrosine; by EPHA2"
FT /evidence="ECO:0000250|UniProtKB:O14493"
FT DISULFID 54..64
FT /evidence="ECO:0000250|UniProtKB:O14493"
SQ SEQUENCE 209 AA; 22165 MW; BB234586BBAEE1F2 CRC64;
MASMGLQVMG IALAVLGWLG AILSCALPMW RVTAFIGSNI VTSQTIWEGL WMNCVVQSTG
QMQCKVYDSL LALPQDLQAA RALIVICIIL AVFGVLLSVV GGKCTNCVDD ESSKAKIMIV
AGVVFLLAGL LVMVPVSWTA NNVIRDFYNP LVASGQKREM GASLYVGWAA AGLLILGGAL
LCFNCPPRND KPYSAKYSAA RSAPASNYV
