CLE12_MEDTR
ID CLE12_MEDTR Reviewed; 81 AA.
AC G7ZZZ3;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 12 {ECO:0000305};
DE Short=MtCLE12 {ECO:0000303|PubMed:20348212};
DE Contains:
DE RecName: Full=CLE12p {ECO:0000303|PubMed:20348212};
DE Flags: Precursor;
GN Name=CLE12 {ECO:0000303|PubMed:20348212};
GN OrderedLocusNames=MTR_4g079630 {ECO:0000312|EMBL:AES89757.1};
GN ORFNames=MtrunA17_Chr4g0040971 {ECO:0000312|EMBL:RHN61839.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=20348212; DOI=10.1104/pp.110.153718;
RA Mortier V., Den Herder G., Whitford R., Van de Velde W., Rombauts S.,
RA D'Haeseleer K., Holsters M., Goormachtig S.;
RT "CLE peptides control Medicago truncatula nodulation locally and
RT systemically.";
RL Plant Physiol. 153:222-237(2010).
RN [5]
RP FUNCTION.
RX PubMed=22168914; DOI=10.1111/j.1365-313x.2011.04881.x;
RA Mortier V., De Wever E., Vuylsteke M., Holsters M., Goormachtig S.;
RT "Nodule numbers are governed by interaction between CLE peptides and
RT cytokinin signaling.";
RL Plant J. 70:367-376(2012).
RN [6]
RP FUNCTION.
RX PubMed=28592666; DOI=10.1104/pp.17.00278;
RA Kassaw T., Nowak S., Schnabel E., Frugoli J.;
RT "ROOT DETERMINED NODULATION1 is required for M. truncatula CLE12, but not
RT CLE13, peptide signaling through the SUNN receptor kinase.";
RL Plant Physiol. 174:2445-2456(2017).
CC -!- FUNCTION: [CLE12p]: Signaling peptide involved in the regulation of
CC nodulation (PubMed:20348212). Moves from root to shoot to function with
CC the receptor kinase SUNN, in a signaling pathway that plays roles
CC during cellular differentiation, both at the onset of nodulation, and
CC later during nodule meristem development and subsequent homeostasis
CC (PubMed:20348212). Interacts with SUNN signaling to control nodule
CC numbers (PubMed:22168914). SUNN is involved in the autoregulation of
CC nodulation (AON), a long distance systemic signaling from root to shoot
CC and back again, which allows legumes to limit the number of root
CC nodules formed based on available nitrogen and previous rhizobial
CC colonization (Probable). {ECO:0000269|PubMed:20348212,
CC ECO:0000269|PubMed:22168914, ECO:0000305|PubMed:22168914}.
CC -!- SUBCELLULAR LOCATION: [CLE12p]: Secreted, extracellular space
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: [CLE12p]: Expressed in young nodules throughout the
CC central tissue (PubMed:20348212). Expressed in the apical region of
CC elongated nodules, corresponding to the meristematic and early
CC infection zones (PubMed:20348212). {ECO:0000269|PubMed:20348212}.
CC -!- INDUCTION: [CLE12p]: Induced in roots during nodulation triggered by
CC low nitrogen and infection with Sinorhizobium meliloti.
CC {ECO:0000269|PubMed:20348212}.
CC -!- PTM: [CLE12p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-76 enhances binding affinity of the CLE12p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- MISCELLANEOUS: [CLE12p]: Overexpression of CL12 in roots almost
CC abolishes nodule formation when inoculated with Sinorhizobium species
CC (PubMed:28592666). Roots of plants silencing CLE12 and CLE13 exhibit
CC increased number of nodules after infection with Sinorhizobium meliloti
CC (PubMed:22168914). {ECO:0000269|PubMed:22168914,
CC ECO:0000269|PubMed:28592666}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; CM001220; AES89757.1; -; Genomic_DNA.
DR EMBL; PSQE01000004; RHN61839.1; -; Genomic_DNA.
DR RefSeq; XP_003607560.1; XM_003607512.2.
DR AlphaFoldDB; G7ZZZ3; -.
DR EnsemblPlants; AES89757; AES89757; MTR_4g079630.
DR Gramene; AES89757; AES89757; MTR_4g079630.
DR KEGG; mtr:MTR_4g079630; -.
DR HOGENOM; CLU_2577465_0_0_1; -.
DR OMA; FFMSPHA; -.
DR OrthoDB; 1924992at2759; -.
DR Proteomes; UP000002051; Chromosome 4.
DR Proteomes; UP000265566; Chromosome 4.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; IEA:InterPro.
DR InterPro; IPR039617; CLAVATA3-CLE.
DR PANTHER; PTHR36016; PTHR36016; 1.
PE 2: Evidence at transcript level;
KW Differentiation; Glycoprotein; Hydroxylation; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..81
FT /note="CLAVATA3/ESR (CLE)-related protein 12"
FT /evidence="ECO:0000255"
FT /id="PRO_0000448632"
FT PEPTIDE 69..81
FT /note="CLE12p"
FT /id="PRO_0000448633"
FT REGION 55..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 73
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 76
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 76
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 81 AA; 9181 MW; 1F6C64DC824AD331 CRC64;
MENSNKVPIS KIGLIMLMIF STFFMSPHAR RLEGGSNIDS QRLLHELMVD RIKQKRSRTD
LEDKAVPGDR LSPGGPNHIH N
