CLE13_ARATH
ID CLE13_ARATH Reviewed; 107 AA.
AC Q6NMF0;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 13 {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE13p {ECO:0000303|PubMed:16489133};
DE Flags: Precursor;
GN Name=CLE13 {ECO:0000303|PubMed:16489133};
GN OrderedLocusNames=At1g73965 {ECO:0000312|Araport:AT1G73965};
GN ORFNames=F2P9 {ECO:0000312|EMBL:AC016662};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [7]
RP FUNCTION.
RX PubMed=16407446; DOI=10.1104/pp.105.072678;
RA Ni J., Clark S.E.;
RT "Evidence for functional conservation, sufficiency, and proteolytic
RT processing of the CLAVATA3 CLE domain.";
RL Plant Physiol. 140:726-733(2006).
RN [8]
RP FUNCTION, AND GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [9]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [10]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [11]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [12]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [13]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE13p]: Extracellular signal peptide that regulates cell
CC fate. Represses root apical meristem maintenance. Regulates the
CC transition of protophloem cells from proliferation to differentiation,
CC thus impinging on postembryonic growth capacity of the root meristem;
CC this signaling pathway requires CRN and CLV2 (PubMed:28607033).
CC {ECO:0000269|PubMed:16407446, ECO:0000269|PubMed:16489133,
CC ECO:0000269|PubMed:16902140, ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE13p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE13p]: Mostly expressed in seedlings, roots,
CC flowers, stems and apex, and, to a lower extent, in leaves and
CC siliques. {ECO:0000269|PubMed:12602871}.
CC -!- PTM: [CLE13p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-102 enhances binding affinity of the CLE13p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AC016662; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE35532.1; -; Genomic_DNA.
DR EMBL; BT011710; AAS49073.1; -; mRNA.
DR EMBL; AK220675; BAD93725.1; -; mRNA.
DR RefSeq; NP_683493.1; NM_148652.3.
DR AlphaFoldDB; Q6NMF0; -.
DR STRING; 3702.AT1G73965.1; -.
DR PaxDb; Q6NMF0; -.
DR PRIDE; Q6NMF0; -.
DR EnsemblPlants; AT1G73965.1; AT1G73965.1; AT1G73965.
DR GeneID; 843734; -.
DR Gramene; AT1G73965.1; AT1G73965.1; AT1G73965.
DR KEGG; ath:AT1G73965; -.
DR Araport; AT1G73965; -.
DR TAIR; locus:504956232; AT1G73965.
DR eggNOG; ENOG502S8TY; Eukaryota.
DR HOGENOM; CLU_145048_0_0_1; -.
DR OMA; DFTPFMK; -.
DR OrthoDB; 1527625at2759; -.
DR PhylomeDB; Q6NMF0; -.
DR PRO; PR:Q6NMF0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6NMF0; baseline and differential.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; ISS:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR InterPro; IPR039618; CLE9/10/11/12/13.
DR PANTHER; PTHR34359; PTHR34359; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..107
FT /note="CLAVATA3/ESR (CLE)-related protein 13"
FT /id="PRO_0000401257"
FT PEPTIDE 96..107
FT /note="CLE13p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401258"
FT REGION 79..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 99
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 102
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 102
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 107 AA; 11952 MW; DD1B6C20FB79FC7B CRC64;
MATTRVSHVL GFLLWISLLI FVSIGLFGNF SSKPINPFPS PVITLPALYY RPGRRALAVK
TFDFTPFLKD LRRSNHRKAL PAGGSEIDPR YGVEKRLVPS GPNPLHH
