CLE14_ARATH
ID CLE14_ARATH Reviewed; 80 AA.
AC Q3ECJ5;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 14 {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE14p {ECO:0000303|PubMed:16489133};
DE Flags: Precursor;
GN Name=CLE14 {ECO:0000303|PubMed:16489133};
GN OrderedLocusNames=At1g63245 {ECO:0000312|Araport:AT1G63245};
GN ORFNames=F9N12 {ECO:0000312|EMBL:AC022355};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [5]
RP FUNCTION.
RX PubMed=16407446; DOI=10.1104/pp.105.072678;
RA Ni J., Clark S.E.;
RT "Evidence for functional conservation, sufficiency, and proteolytic
RT processing of the CLAVATA3 CLE domain.";
RL Plant Physiol. 140:726-733(2006).
RN [6]
RP GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [7]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [8]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [9]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [10]
RP DEVELOPMENTAL STAGE, AND FUNCTION.
RX PubMed=20697738; DOI=10.1007/s00425-010-1236-4;
RA Meng L., Feldman L.J.;
RT "CLE14/CLE20 peptides may interact with CLAVATA2/CORYNE receptor-like
RT kinases to irreversibly inhibit cell division in the root meristem of
RT Arabidopsis.";
RL Planta 232:1061-1074(2010).
RN [11]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [12]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND INTERACTION WITH CLV2 AND
RP PEPR2.
RX PubMed=28586647; DOI=10.1016/j.devcel.2017.05.009;
RA Gutierrez-Alanis D., Yong-Villalobos L., Jimenez-Sandoval P.,
RA Alatorre-Cobos F., Oropeza-Aburto A., Mora-Macias J., Sanchez-Rodriguez F.,
RA Cruz-Ramirez A., Herrera-Estrella L.;
RT "Phosphate starvation-dependent iron mobilization induces CLE14 expression
RT to trigger root meristem differentiation through CLV2/PEPR2 signaling.";
RL Dev. Cell 41:555-570(2017).
RN [13]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE14p]: Extracellular signal peptide that regulates cell
CC fate. Represses root apical meristem maintenance. Acts as an elicitor
CC of the root meristem differentiation through the CLV2/CRN complex
CC signaling pathway (PubMed:20697738, PubMed:28586647). Inhibits
CC irreversibly root growth by reducing cell division rates in the root
CC apical meristem (PubMed:20697738, PubMed:28586647). Regulates the
CC transition of protophloem cells from proliferation to differentiation,
CC thus impinging on postembryonic growth capacity of the root meristem;
CC this signaling pathway requires CRN and CLV2 (PubMed:28607033).
CC {ECO:0000269|PubMed:16407446, ECO:0000269|PubMed:16902140,
CC ECO:0000269|PubMed:20697738, ECO:0000269|PubMed:28586647,
CC ECO:0000269|PubMed:28607033}.
CC -!- SUBUNIT: [CLE14p]: Interacts with the extracellular leucine-rich repeat
CC region of CLV2 and PEPR2. {ECO:0000303|PubMed:28586647}.
CC -!- SUBCELLULAR LOCATION: [CLE14p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE14p]: Mostly expressed in roots, and, to a
CC lower extent, in seedlings and leaves (PubMed:12602871). Expressed in
CC the primary root tip under Pi deficiency (PubMed:28586647).
CC {ECO:0000269|PubMed:12602871, ECO:0000269|PubMed:28586647}.
CC -!- DEVELOPMENTAL STAGE: [CLE14p]: Expressed specifically in
CC differentiating cells of the root. {ECO:0000269|PubMed:20697738}.
CC -!- INDUCTION: [CLE14p]: Induced by Pi starvation.
CC {ECO:0000269|PubMed:28586647}.
CC -!- PTM: [CLE14p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-74 enhances binding affinity of the CLE14p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AC022355; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE34075.1; -; Genomic_DNA.
DR RefSeq; NP_001319300.1; NM_001334074.1.
DR AlphaFoldDB; Q3ECJ5; -.
DR STRING; 3702.AT1G63245.1; -.
DR PaxDb; Q3ECJ5; -.
DR PRIDE; Q3ECJ5; -.
DR EnsemblPlants; AT1G63245.1; AT1G63245.1; AT1G63245.
DR GeneID; 28717391; -.
DR Gramene; AT1G63245.1; AT1G63245.1; AT1G63245.
DR KEGG; ath:AT1G63245; -.
DR Araport; AT1G63245; -.
DR TAIR; locus:504956189; AT1G63245.
DR HOGENOM; CLU_172158_0_0_1; -.
DR InParanoid; Q3ECJ5; -.
DR OMA; PNIMTAT; -.
DR OrthoDB; 1600241at2759; -.
DR PhylomeDB; Q3ECJ5; -.
DR PRO; PR:Q3ECJ5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q3ECJ5; baseline and differential.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; IPI:UniProtKB.
DR GO; GO:0005102; F:signaling receptor binding; IPI:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IDA:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR GO; GO:1900055; P:regulation of leaf senescence; IMP:TAIR.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IGI:TAIR.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..80
FT /note="CLAVATA3/ESR (CLE)-related protein 14"
FT /id="PRO_0000401259"
FT PEPTIDE 68..79
FT /note="CLE14p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401260"
FT MOD_RES 71
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 74
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 74
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 80 AA; 9037 MW; 794EF20E003BC7FC CRC64;
MKVWSQRLSF LIVMIFILAG LHSSSAGRKL PSMTTTEEFQ RLSFDGKRIL SEVTADKKYD
RIYGASARLV PKGPNPLHNK
