CLE18_ARATH
ID CLE18_ARATH Reviewed; 101 AA.
AC Q3ECH9;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 18 {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE18p {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE18 C-terminus {ECO:0000303|PubMed:22307643};
DE Flags: Precursor;
GN Name=CLE18 {ECO:0000303|PubMed:16489133};
GN OrderedLocusNames=At1g66145 {ECO:0000312|Araport:AT1G66145};
GN ORFNames=F15E12 {ECO:0000312|EMBL:AC026480};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [5]
RP FUNCTION, AND GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [6]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [7]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [8]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [9]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [10]
RP FUNCTION, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=22307643; DOI=10.1073/pnas.1119864109;
RA Meng L., Buchanan B.B., Feldman L.J., Luan S.;
RT "CLE-like (CLEL) peptides control the pattern of root growth and lateral
RT root development in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:1760-1765(2012).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE18 C-terminus]: Root growth factor that regulates the
CC pattern of root growth and lateral root development by modulating the
CC length and the number of cortical cells in the root apical meristem
CC (RAM), and the anticlinal asymmetric cell divisions in lateral root
CC initiation cells. {ECO:0000269|PubMed:22307643}.
CC -!- FUNCTION: [CLE18p]: Extracellular signal peptide that regulates cell
CC fate (PubMed:16489133). Represses root apical meristem maintenance
CC (PubMed:16902140). Root growth factor that regulates the pattern of
CC root growth and lateral root development (PubMed:22307643). Regulates
CC the transition of protophloem cells from proliferation to
CC differentiation, thus impinging on postembryonic growth capacity of the
CC root meristem; this signaling pathway requires CRN and CLV2
CC (PubMed:28607033). {ECO:0000269|PubMed:16489133,
CC ECO:0000269|PubMed:16902140, ECO:0000269|PubMed:22307643,
CC ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE18p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- SUBCELLULAR LOCATION: [CLE18 C-terminus]: Secreted
CC {ECO:0000250|UniProtKB:Q3E880}.
CC -!- TISSUE SPECIFICITY: [CLE18p]: Expressed in roots, leaves, siliques and
CC seedlings. {ECO:0000269|PubMed:12602871}.
CC -!- PTM: [CLE18 C-terminus]: The tyrosine sulfation is critical for the
CC function of the peptide. {ECO:0000250|UniProtKB:Q3E880}.
CC -!- PTM: [CLE18p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-43 enhances binding affinity of the CLE18p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AC026480; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE34467.1; -; Genomic_DNA.
DR RefSeq; NP_683472.1; NM_148631.1.
DR AlphaFoldDB; Q3ECH9; -.
DR STRING; 3702.AT1G66145.1; -.
DR PaxDb; Q3ECH9; -.
DR PRIDE; Q3ECH9; -.
DR EnsemblPlants; AT1G66145.1; AT1G66145.1; AT1G66145.
DR GeneID; 842929; -.
DR Gramene; AT1G66145.1; AT1G66145.1; AT1G66145.
DR KEGG; ath:AT1G66145; -.
DR Araport; AT1G66145; -.
DR TAIR; locus:504956311; AT1G66145.
DR HOGENOM; CLU_2295554_0_0_1; -.
DR OMA; HWIGVEE; -.
DR OrthoDB; 1816863at2759; -.
DR PRO; PR:Q3ECH9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; IPI:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0009786; P:regulation of asymmetric cell division; ISS:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR GO; GO:2000023; P:regulation of lateral root development; IDA:UniProtKB.
DR GO; GO:0010082; P:regulation of root meristem growth; IDA:UniProtKB.
DR GO; GO:2000067; P:regulation of root morphogenesis; IDA:UniProtKB.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal; Sulfation.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..101
FT /note="CLAVATA3/ESR (CLE)-related protein 18"
FT /id="PRO_0000401265"
FT PEPTIDE 37..48
FT /note="CLE18p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401266"
FT PEPTIDE 75..87
FT /note="CLE18 C-terminus"
FT /evidence="ECO:0000269|PubMed:22307643"
FT /id="PRO_0000451984"
FT REGION 37..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 40
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 43
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 76
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT MOD_RES 84
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 43
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 101 AA; 11363 MW; 87673266EE3B3225 CRC64;
MHLLKGGVVL IITLILFLIT SSIVAIREDP SLIGVDRQIP TGPDPLHNPP QPSPKHHHWI
GVEENNIDRS WNYVDYESHH AHSPIHNSPE PAPLYRHLIG V
